| 摘要 | 第1-10页 |
| ABSTRACT | 第10-17页 |
| Review： The Research of Lipid Nutrition and Metabolism | 第17-43页 |
| 1 Introduction | 第17-20页 |
| 2 Aims of the study | 第20-21页 |
| 3 Background | 第21-41页 |
| ·Definitions, digestion and absorption of lipids | 第21-22页 |
| ·Extracellular transport of lipids | 第22-24页 |
| ·Trans-membrane fatty acid uptake | 第24-31页 |
| ·Intracellular metabolism of fatty acids | 第31-40页 |
| ·Lipid peroxidation: n-3 HUFAs and the oxidative stress | 第40-41页 |
| 4.M ethodological considerations | 第41-43页 |
| Chapter 1 Influence of fish oil on growth and lipid metabolism in Common carp (Cyprinus carpio L.) | 第43-50页 |
| 1 Materials and methods | 第43-46页 |
| ·Design of Experiment | 第43-44页 |
| ·Trial 1, The effect of fish oil on growth, lipid metabolism of common carp | 第44-45页 |
| ·Trial 2：Effect of DHA administration on enzyme of lipid metabolism in common carp | 第45-46页 |
| ·Statistics | 第46页 |
| 2 Results | 第46-48页 |
| ·Effect of fish oil on growth | 第46页 |
| ·Effect of fish oil on biological parameters | 第46-47页 |
| ·Effect of fish oil on enzyme of lipid metabolism | 第47页 |
| ·Effect of HUFA administration on activity of malate dehydrogenase in hepatopancreas | 第47-48页 |
| 3 Discussion | 第48-50页 |
| ·Effect of different dietary oils on growth of fish | 第48页 |
| ·Effect of fish oil and HUFA on lipid metabolism of fish | 第48-50页 |
| Chapter 2 Effect of DHA on anti-oxidation capacity in Common carp (Cyprinus carpio. | 第50-58页 |
| 1 Materials and Methods | 第50-53页 |
| ·Design of Experiment | 第50-51页 |
| ·Materials and Methods | 第51-53页 |
| ·Data presentation and statistic analysis | 第53页 |
| 2 Results | 第53-55页 |
| ·Effect of DHA administration on T-AOC in hepatopancreas | 第53-54页 |
| ·Effect of DHA feeding on anti-oxidation capacity | 第54-55页 |
| ·Effect of DHA on anti-oxidation capacity in hepatocyte | 第55页 |
| 3 Discussion | 第55-58页 |
| ·Effect of DHA administration, long term feeding and cell culture (in vitro) on anti-oxidation capacity in Common carp | 第55-57页 |
| ·Effect of dietary oil levels on anti-oxidation capacity | 第57页 |
| ·Consideration of experiment methods | 第57-58页 |
| Chapter 3 Uptake and intracellular metabolism of long chain fatty acids in Atlantic salmon (Salmo salar L.) hepatocytes | 第58-75页 |
| 1 Materials | 第59-60页 |
| 2 Methods | 第60-63页 |
| ·Preparation of hepatocytes | 第60-61页 |
| ·OA uptake depending on time and OA concentration | 第61页 |
| ·Inhibition of OA uptake | 第61页 |
| ·Uptake of long-chain FAs | 第61页 |
| ·Incorporation of fatty acids into lipid classes | 第61-62页 |
| ·Assay of hepatocytes β-oxidation activity | 第62页 |
| ·Apoptosis test: MTT test and heocst staining test | 第62-63页 |
| ·Statistical analysis | 第63页 |
| 3 Results | 第63-69页 |
| ·OA uptake - time course study | 第63-64页 |
| ·OA uptake -concentration study | 第64-65页 |
| ·Effects of inhibitors on OA uptake | 第65-66页 |
| ·Uptake of long-chain FAs into hepatocytes | 第66-67页 |
| ·Distribution of fatty acids into cellular lipids | 第67-68页 |
| ·Fatty acids oxidation | 第68-69页 |
| ·Cell viability | 第69页 |
| 4 Discussion | 第69-75页 |
| Chapter 4 Replacing dietary fish oil with vegetable oil affect oleic acid uptake and metabolism in hepatocytes of Atlantic salmon (Salmo salar L.) | 第75-96页 |
| 1 Materials | 第77-78页 |
| 2 Methods | 第78-83页 |
| ·Tailoring of fatty acid composition in Atlantic salmon hepatocytes | 第78页 |
| ·Fatty acid composition of liver and diets | 第78-79页 |
| ·Lipid class composition of liver and diets | 第79页 |
| ·Preparation of hepatocytes | 第79-80页 |
| ·Uptake assays and hepatocyte harvesting | 第80页 |
| ·Analysis of β-oxidation activity | 第80页 |
| ·Incorporation of OA into lipid classes | 第80-81页 |
| ·Quantitative RT real time-PCR | 第81-82页 |
| ·MTT test | 第82-83页 |
| ·Heocst staining test | 第83页 |
| ·Data presentation and statistical analysis | 第83页 |
| 3 Results | 第83-92页 |
| ·The effect of dietary oil on fatty acid composition of liver | 第83-85页 |
| ·OA uptake | 第85-86页 |
| ·Incorporation of OA into cellular lipid classes and β-oxidation products | 第86-88页 |
| ·Relative expression of cellular fatty acid transporter and metabolic genes | 第88-91页 |
| ·Relative expression of stress marker genes | 第91-92页 |
| ·Effects of inhibitors on cell viability of hepatocytes | 第92页 |
| 4 Discussion | 第92-96页 |
| Conclusion | 第96-98页 |
| Innovation | 第98页 |
| Further perspectives | 第98-99页 |
| Reference | 第99-126页 |
| Index | 第126-127页 |
| Acknowledgement | 第127-128页 |
| Information of the auther | 第128页 |
