| 摘要 | 第1-5页 |
| ABSTRACT | 第5-7页 |
| Content | 第7-9页 |
| Chapter 1 Introductions | 第9-24页 |
| ·Structure, function, formation and regulation of biological iron-sulfur clusters | 第9-22页 |
| ·Structures and Properties of Biological [Fe-S] Clusters | 第9-12页 |
| ·Functions of Biological [Fe-S] Clusters | 第12-15页 |
| ·Formation of Biological [Fe-S] Clusters | 第15-19页 |
| ·IscR acts as an represses expression factor in response for the isc operon encoding [Fe-S] cluster assembly proteins in Escherichia coli | 第19-20页 |
| ·IscR acts as an activator in response to oxidative stress for the suf operon encoding Fe-S assembly proteins | 第20-22页 |
| ·The objective and main content of the present study | 第22-24页 |
| ·The objective of the present study | 第22-23页 |
| ·The main content of the present study | 第23-24页 |
| Chapter 2 Expression, purification and characterization of an iron-sulfur cluster assembly Regulator IscR from A. ferrooxidans | 第24-34页 |
| ·Materials | 第25-26页 |
| ·Methods | 第26-28页 |
| ·Cloning of the IscR gene from A. ferrooxidans | 第26-27页 |
| ·Expression of recombinant protein of IscR from A. ferrooxidans | 第27页 |
| ·Purification of Isc R from A. ferrooxidans | 第27-28页 |
| ·UV-Vis scanning and EPR spectra | 第28页 |
| ·Determination of iron and sulfur content | 第28页 |
| ·Results and discussion | 第28-33页 |
| ·Cloning of the IscR gene from A. ferrooxidans | 第28-30页 |
| ·Expression and purification of IscR from A. ferrooxidans | 第30-31页 |
| ·UV-scanning and EPR spectra of the IscR from A. ferrooxidans | 第31-33页 |
| ·Conclusion | 第33-34页 |
| Chapter 3 Site-directed mutagenesis of an iron-sulfur cluster assembly Regulator IscR from A. ferrooxidans and Iron-sulfur cluster assembly in apoIscR in vitro | 第34-48页 |
| ·Materials | 第34-35页 |
| ·Methods | 第35-38页 |
| ·Construction of A. ferrooxidans IscR mutant plasmids | 第35-36页 |
| ·Expression of the IscR of mutant proteins | 第36页 |
| ·Purification of the IscR of the mutant proteins | 第36-37页 |
| ·UV-Vis Scanning and EPR Spectra | 第37页 |
| ·Preparation of apoIscR | 第37页 |
| ·Iron-sulfur cluster assembly of IscR in vitro | 第37页 |
| ·Determination of iron and sulifde content of IscR | 第37-38页 |
| ·Results and discussion | 第38-47页 |
| ·The conserved residues for iron-sulfur cluster binding | 第38-39页 |
| ·Expression and Purification of IscR Mutant Proteins | 第39-40页 |
| ·Iron and sulfide content of IscR mutant proteins | 第40-41页 |
| ·UV-Scanning and EPR Spectra of IscR Mutant Proteins | 第41-43页 |
| ·The [2Fe2S] cluster assembly in IscR in vivo required scaffold proteins | 第43-47页 |
| ·Conclusion | 第47-48页 |
| Chapter 4 Conclusion | 第48-49页 |
| Abbreviations | 第49-50页 |
| References | 第50-56页 |
| Acknowledgements | 第56-58页 |
| Main achievements | 第58页 |
