| ABSTRACT | 第9-10页 |
| Abbreviation | 第11-12页 |
| CHAPTER Ⅰ INTRODUCTION | 第12-15页 |
| CHAPTER Ⅱ LITERATURE REVIEW | 第15-22页 |
| 2.1 Water Buffalo and Dairy Cow | 第15-16页 |
| 2.2 Candidate Gene | 第16-19页 |
| 2.2.1 PHF 20 Gene | 第16-17页 |
| 2.2.2 CYHR 1 Gene | 第17页 |
| 2.2.3 LOC 514211 Gene | 第17页 |
| 2.2.4 MAN1A1 Gene | 第17-18页 |
| 2.2.5 SPATC1 Gene | 第18-19页 |
| 2.3 Polymerase Chain Reaction and Restriction Fragment Length Polymorphism (PCR-RFLP) | 第19-20页 |
| 2.4 Single Nucleotide Polymorphism (SNP) | 第20页 |
| 2.5 SNP Association Studies in Milk production | 第20-21页 |
| 2.6 SNP Association Studies in Reproduction | 第21-22页 |
| CHAPTER Ⅲ MATERIAL AND METHODE | 第22-29页 |
| 3.1 Material Research | 第22-24页 |
| 3.1.1 DNA Samples | 第22页 |
| 3.1.2 Equipment and Supplies | 第22-23页 |
| 3.1.3 Reagents and Solutions | 第23-24页 |
| 3.2 Method | 第24-26页 |
| 3.2.1 Blood sample | 第24页 |
| 3.2.2 DNA extraction | 第24-25页 |
| 3.2.3 SNP Detection and genotyping | 第25-26页 |
| 3.3 Statistical Analysis | 第26-29页 |
| CHAPTER Ⅳ RESULTS AND DISCUSSION | 第29-50页 |
| 4.1 Identification genotype of SNPs of Candidate Genes in Dairy Cow and Water Buffalo | 第29-36页 |
| 4.1.1 PHF20 rs 43713830 | 第29-31页 |
| 4.1.1.1 Genotype of SNP in Dairy Cow | 第29页 |
| 4.1.1.2 Genotypes Frequency of SNP in Dairy Cow | 第29-30页 |
| 4.1.1.3 Genotypes and Frequency of SNP in Water Buffalo | 第30-31页 |
| 4.1.2 CYHR1 rs 109968515 | 第31-32页 |
| 4.1.2.1 Genotypes of SNP in Dairy Cow | 第31页 |
| 4.1.2.2 Genotypes Frequency of SNP in Dairy Cow | 第31页 |
| 4.1.2.3 Genotypes and Frequency of SNP in Water Buffalo | 第31-32页 |
| 4.1.3 LOC514211 rs 42688595 | 第32-33页 |
| 4.1.3.1 Genotypes of SNP in Dairy Cow | 第32-33页 |
| 4.1.3.2 Genotypes Frequency of SNP in Dairy Cow | 第33页 |
| 4.1.3.3 Genotypes and Frequency of SNP in Water Buffalo | 第33页 |
| 4.1.4 MAN1A1 rs 42855643 | 第33-35页 |
| 4.1.4.1 Genotypes of SNP in Dairy Cow | 第33-34页 |
| 4.1.4.2 Genotypes Frequency of SNP in Dairy Cow | 第34页 |
| 4.1.4.3 Genotypes and Frequency of SNP in Water Buffalo | 第34-35页 |
| 4.1.5 SPATC1 rs 41629750 | 第35-36页 |
| 4.1.5.1 Genotypes of SNP in Dairy Cow | 第35页 |
| 4.1.5.2 Genotypes Frequency of SNP in Dairy Cow | 第35页 |
| 4.1.5.3 Genotypes and Frequency of SNP in Water Buffalo | 第35-36页 |
| 4.2 Association Analysis of SNP Candidate Gene with Milk Yield in Dairy Cow | 第36-39页 |
| 4.2.1 Association Analysis of SNP Candidate Gene with 305 Days with Milk (305 DM) | 第36-37页 |
| 4.2.2 Association Analysis of SNP Candidate Gene with Total Milk Yield | 第37-39页 |
| 4.3 Analysis Association of SNP Candidate Gene with Milk Quality in Dairy Cow | 第39-42页 |
| 4.3.1 Analysis Association of SNP Candidate Gene with Fat Milk Rate | 第39-40页 |
| 4.3.2. Analysis Association of SNP Candidate Gene with Protein Milk Rate | 第40页 |
| 4.3.3 Analysis Association of SNP Candidate Gene with Total Fat Yield | 第40-41页 |
| 4.3.4 Analysis Association of SNP Candidate Gene with Total Protein Yield | 第41-42页 |
| 4.4 Analysis Association of SNP Candidate Gene with Reproductive Traits in Dairy Cow | 第42-48页 |
| 4.4.1. Analysis Association of SNP Candidate Gene with Days to First Service | 第42-44页 |
| 4.4.2. Analysis Association of SNP Candidate Gene with Days Open | 第44-45页 |
| 4.4.3 Analysis Association of SNP Candidate Gene with Birth Weight | 第45-46页 |
| 4.4.4 Analysis Association of SNP Candidate Gene with Calving Interval | 第46-47页 |
| 4.4.5. Analysis Association of SNP Candidate Gene with Age of Calving | 第47-48页 |
| 4.5 Analysis Association of SNP Candidate Gene with Milk Production and Reproductionin Water Buffalo | 第48-50页 |
| CHAPTER Ⅴ DISCUSSION | 第50-58页 |
| 5.1 Identification genotype of SNPs of Candidate Genes in Dairy Cow and Water Buffalo | 第50-52页 |
| 5.2 Association Analysis of SNP Candidate Gene with Milk Yield in Dairy Cow | 第52-53页 |
| 5.3 Association Analysis of SNP Candidate Gene with Milk Quality in Dairy Cow | 第53-54页 |
| 5.4 Association Analysis of SNP Candidate Gene with Reproduction in Dairy Cow | 第54-56页 |
| 5.5 Analysis of SNP Candidate Gene with Milk Production and Reproductive Trait in Water Buffalo | 第56-58页 |
| CHAPTER Ⅵ CONCLUSION | 第58-59页 |
| CHAPTER Ⅶ FURTHER RESEARCH | 第59-60页 |
| LITERATURE CITED | 第60-67页 |
| ACKNOWLEDGEMENTS | 第67-68页 |
