| Abbreviation | 第6-9页 |
| 摘要 | 第9-11页 |
| Abstract | 第11-12页 |
| 1 Introduction | 第13-42页 |
| 1.1 Rapeseed relationship to brassica species, its origin and genome components | 第17-22页 |
| 1.1.1 Genus Brassica in Brassicaceae and U’s triangle | 第17-19页 |
| 1.1.2 Rapeseed: genetics and origin | 第19-21页 |
| 1.1.3 Genomic constituents of B.napus, their genetics and genomics | 第21-22页 |
| 1.2 B.napus genome Complexity | 第22-29页 |
| 1.2.1 Whole genome triplication (WGT) in brassica | 第22-23页 |
| 1.2.2 Genome down sizing | 第23-25页 |
| 1.2.3 Subgenome dominance in polyploidy B.napus | 第25-27页 |
| 1.2.4 Homeologous pairing and Chromosomal rearrangement in B.napus | 第27-29页 |
| 1.3 Role of comparative mapping to dissect genomic components of B.napus | 第29-35页 |
| 1.3.1 Comparative mapping between Arabidopsis and B.napus | 第30-33页 |
| 1.3.2 Comparative mapping between B.napus and ancestors B.rapa and B.oleracea | 第33-35页 |
| 1.4 Advent of molecular marker technology and application in B.napus | 第35-37页 |
| 1.5 INDELs variations in genomes | 第37-41页 |
| 1.5.1 Characteristics of INDELs | 第38-39页 |
| 1.5.2 Mechanism of INDELs mutation | 第39页 |
| 1.5.3 Factors effecting density and distribution of INDEL across the genomes | 第39-41页 |
| 1.6 Objectives | 第41-42页 |
| 2 Materials and methods | 第42-51页 |
| 2.1 Plant materials | 第42-43页 |
| 2.2 DNA extraction and sample preparation | 第43-44页 |
| 2.3 Construction of whole-genome shotgun libraries and sequencing | 第44-45页 |
| 2.4 Procedures for INDEL discovery | 第45-47页 |
| 2.5 Development of single-locus INDELs markers | 第47-48页 |
| 2.6 Experimental validation of INDELs polymorphism | 第48-49页 |
| 2.7 Polyacrylamide gel electrophoresis | 第49-50页 |
| 2.7.1 Gel preparation | 第49-50页 |
| 2.7.2 PCR product separation by electrophoresis | 第50页 |
| 2.7.3 Silver staining | 第50页 |
| 2.8 INDELs genetic linkage map construction | 第50-51页 |
| 3 Results | 第51-69页 |
| 3.1 Whole-genome re-sequencing of the 22 ILs | 第51-53页 |
| 3.2 Identification of short INDEL polymorphisms | 第53-55页 |
| 3.3 Genomic distribution of INDEL variations | 第55-58页 |
| 3.4 Distribution of INDEL relative to genes and transposable elements | 第58-60页 |
| 3.5 Development of INDEL markers | 第60-61页 |
| 3.6 Genetic mapping of INDELs | 第61-69页 |
| 4 Discussions | 第69-76页 |
| 4.1 Certainty of B. napus genome sequencing | 第69-70页 |
| 4.2 Challenges to B.napus sequencing and polymorphism detection | 第70-72页 |
| 4.3 Identification of INDEL polymorphism | 第72页 |
| 4.4 Characterization of INDEL polymorphism | 第72-73页 |
| 4.5 Role of INDEL in genome complexity and variation | 第73-74页 |
| 4.6 INDEL genotyping and mapping accuracy | 第74-76页 |
| Summary | 第76-80页 |
| References | 第80-101页 |
| Appendix | 第101-104页 |
| Sequence location of INDEL markers in B.napus genome and primer sequence information | 第104-128页 |
| ACKNOWLEDGEMENTS | 第128-129页 |
