| DEDICATION | 第3-4页 |
| ABSTRACT | 第4-7页 |
| 摘要 | 第8-22页 |
| LIST OF ABBREVIATIONS & SYMBOLS | 第22-23页 |
| CHAPTER1: LITERATURE REVIEW | 第23-36页 |
| 1.1 INTRODUCTION | 第23-24页 |
| 1.2 THE CHICKEN GROWTH HORMONE GENE (GH) | 第24-25页 |
| 1.2.1 The GH, Somatotropic and Thyrotropic Axes | 第25页 |
| 1.3 THE INSULIN-LIKE GROWTH FACTOR-Ⅰ GENE (IGF-Ⅰ) | 第25-27页 |
| 1.3.1 The Insulin-like growth Factor-Ⅰ gene (IGF-Ⅰ) axis | 第26-27页 |
| 1.4 THE INSULIN-LIKE GROWTH FACTOR-Ⅱ GENE (IGF-Ⅱ) | 第27-28页 |
| 1.4.1 The Insulin-like growth Factor-Ⅱ gene (IGF-Ⅱ) axis | 第28页 |
| 1.5 THE GROWTH HORMONE (GH) AND BONE | 第28-31页 |
| 1.5.1 Effects of GH and IGF-Ⅰ on Longitudinal Bone Growth | 第29页 |
| 1.5.2 The physiological role of GH and IGF-Ⅰ for bone growth | 第29-31页 |
| REFERENCE | 第31-36页 |
| CHAPTER 2: ASSOCIATION ANALYSIS WITH GROWTH AND REPRODUCTIVE TRAITS AND THE EXPRESSION PROFILES OF THE GH GENE IN JINGHAI YELLOW CHICKENS | 第36-62页 |
| 2.1 INTRODUCTION | 第37-38页 |
| 2.2 MATERIAL AND METHODs | 第38-41页 |
| 2.2.1 Population and samples collection | 第38-39页 |
| 2.2.2 DNA extraction and genotyping | 第39页 |
| 2.2.3 Primer design and PCR amplification | 第39-40页 |
| 2.2.4 Single-strand conformation polymorphism (SSCP) and sequencing | 第40页 |
| 2.2.5 Expression profiles analysis | 第40页 |
| 2.2.6 Statistical analysis | 第40-41页 |
| 2.3 RESULTS | 第41-56页 |
| 2.3.1 Polymorphisms of GH and genetic variation analysis | 第41-45页 |
| 2.3.2 Allele and genotype frequency of SNP in GH gene | 第45-46页 |
| 2.3.3 Association analysis with growth traits in CH gene | 第46-47页 |
| 2.3.4 Association analysis with reproductive traits in CH gene | 第47-49页 |
| 2.3.5 Association analysis of interaction effect for growth traits in CH gene | 第49页 |
| 2.3.6 Association analysis of interaction effect for reproductive traits | 第49-50页 |
| 2.3.7 Expression profile of GH gene in male and female Jinghai yellow chickens | 第50-52页 |
| 2.3.8 Expression regularity of GH gene in male and female Jinghai yellow chickens | 第52-56页 |
| 2.4 DISCUSSION | 第56-59页 |
| REFERENCE | 第59-62页 |
| CHAPTER 3: ASSOCIATION ANALYSIS WITH GROWTH AND REPRODUCTIVE TRAITS AND THE EXPRESSION PROFILES OF THE IGF-Ⅰ GENE IN JINGHAI YELLOW CHICKENS | 第62-82页 |
| 3.1 INTRODUCTION | 第62-64页 |
| 3.2 MATERIAL AND METHODS | 第64-67页 |
| 3.2.1 Population and samples collection | 第64页 |
| 3.2.2 DNA extraction and genotyping | 第64页 |
| 3.2.3 Primer design and PCR amplification | 第64-65页 |
| 3.2.4 Single-strand conformation polymorphism (SSCP) and sequencing | 第65页 |
| 3.2.5 Expression profiles analysis | 第65-66页 |
| 3.2.6 Statistical analysis | 第66-67页 |
| 3.3 RESULTs | 第67-76页 |
| 3.3.1 Polymorphisms of IGF-Ⅰ and genetic variation analysis | 第67-69页 |
| 3.3.2 Allele and genotype frequencies of SNP in IGF-Ⅰ gene | 第69-70页 |
| 3.3.3 Association analysis with growth traits | 第70页 |
| 3.3.4 Association analysis with reproductive traits | 第70-71页 |
| 3.3.5 Expression profile of IGF-Ⅰ gene in male and female Jinghai yellow chickens | 第71-73页 |
| 3.3.6 Expression regularity of IGF-Ⅰ gene in male and female Jinghai yellow chickens | 第73-76页 |
| 3.4 DISCUSSION | 第76-79页 |
| REFERENCE | 第79-82页 |
| CHAPTER 4: ASSOCIATION ANALYSIS WITH GROWTH AND REPRODUCTIVE TRAITS AND THE EXPRESSION PROFILES OF THE IGF-Ⅱ GENE IN JINGHAI YELLOW CHICKENS | 第82-106页 |
| 4.1 INTRODUCTION | 第83-84页 |
| 4.2 MATERIAL AND METHODS | 第84-87页 |
| 4.2.1 Population and samples collection | 第84页 |
| 4.2.2 DNA extraction and genotyping | 第84页 |
| 4.2.3 Primer design and PCR amplification | 第84-85页 |
| 4.2.4 Single-strand conformation polymorphism (SSCP) and sequencing | 第85-86页 |
| 4.2.5 Expression profiles analysis | 第86页 |
| 4.2.6 Statistical analysis | 第86-87页 |
| 4.3 RESULTS | 第87-101页 |
| 4.3.1 Polymorphisms of IGF-Ⅱ and genetic variation analysis | 第87-90页 |
| 4.3.2 Allele and genotype frequency of SNP in IGF-Ⅱ gene | 第90-91页 |
| 4.3.3 Association analysis with growth traits | 第91-92页 |
| 4.3.4 Association analysis with reproductive traits | 第92-93页 |
| 4.3.5 Association analysis of interaction effect for growth traits | 第93-94页 |
| 4.3.6 Association analysis of interaction effect for Reproductive traits | 第94-95页 |
| 4.3.7 Expression profiles of IGF-Ⅱ gene in male and female Jinghai yellow chickens | 第95-97页 |
| 4.3.8 Expression regularity of IGF-Ⅱ gene in male and female Jinghai yellow chickens | 第97-101页 |
| 4.4 DISCUSSION | 第101-104页 |
| REFERENCE | 第104-106页 |
| CHAPTER 5: ASSOCIATION ANALYSIS OF INTERACTION EFFECT FOR GROWTH AND REPRODUCTIVE TRAITS AMONG THE DETECTED MUTATIONS IN THE GH, IGF-Ⅰ AND IGF-Ⅱ GENES IN JINGHAI YELLOW CHICKENS | 第106-136页 |
| 5.1 INTRODUCTION | 第107-108页 |
| 5.2 MATERIAL AND METHODS | 第108-109页 |
| 5.2.1 Statistical analysis | 第108-109页 |
| 5.3 RESULTS | 第109-129页 |
| 5.3.1 Association analysis of interaction effect for growth traits between detected mutations in the GH and IGF-Ⅰ genes | 第109-110页 |
| 5.3.2 Association analysis of interaction effect for growth traits between detected mutations in the GH and IGF-Ⅱ genes | 第110-113页 |
| 5.3.3 Association analysis of interaction effect for growth traits between detected mutations in the IGF-Ⅰ and IGF-Ⅱ genes | 第113-115页 |
| 5.3.4 Association analysis of interaction effect for reproductive traits between detected mutations in the GH and IGF-Ⅰ genes | 第115-117页 |
| 5.3.5 Association analysis of interaction effect for reproductive traits between detected mutations in the GH and IGF-Ⅱ genes | 第117-120页 |
| 5.3.6 Association analysis of interaction effect for reproductive traits between detected mutations in the IGF-Ⅰ and IGF-Ⅱ genes | 第120-121页 |
| 5.3.7 The comparison of expression profile between the GH and IGF-Ⅰ genes in male and female Jinghai yellow chickens | 第121-123页 |
| 5.3.8 The comparison of expression profile between the GH and IGF-Ⅱ genes in maleand female Jinghai yellow chickens | 第123-124页 |
| 5.3.9 The comparison of expression profile between the IGF-Ⅰand IGF-Ⅱ genes in maleand female Jinghai yellow chickens | 第124-126页 |
| 5.3.10 The comparison of expression profile between the GH and IGF-Ⅰ genes in Breastand Leg muscle for both male and female Jinghai yellow chickens | 第126-127页 |
| 5.3.11 The comparison of expression profile between the GH and IGF-Ⅱ genes in Breastand Leg muscle for both male and female Jinghai yellow chickens | 第127-128页 |
| 5.3.12 The comparison of expression profile between the IGF-Ⅰ and IGF-Ⅱ genes inBreast and Leg muscle for both male and female Jinghai yellow chickens | 第128-129页 |
| 5.4 DISGUSSION | 第129-133页 |
| REFERENCE | 第133-136页 |
| CONCLUSION | 第136-138页 |
| LIST OF PUBLICATIONS | 第138-139页 |
