| Summary in Chinese | 第1-6页 |
| Summary in English | 第6-10页 |
| Chapter 1 Introduction of the Potential of Plants as ‘Bioreactors | 第10-50页 |
| ·Background | 第10-11页 |
| ·Plant Based Production Systems | 第11-13页 |
| ·Prospects For The Protein Production Systems | 第13-48页 |
| ·Future For Plant Based Production Systems | 第48-49页 |
| ·Objective Of Our Project | 第49-50页 |
| Chapter 2 Materials and Methods | 第50-64页 |
| ·Plant Materials | 第50页 |
| ·Methods and Protocols | 第50-64页 |
| Chapter 3 Construction of Plasmids | 第64-90页 |
| ·Basic Construct Design | 第64页 |
| ·Double355GFP | 第64-71页 |
| ·p850021 | 第71-73页 |
| ·-90-GFP | 第73-82页 |
| ·pGreen: Binary Vectors | 第82-87页 |
| ·AGL1pSoup-pG0020 and AGL1pSoup-pG0023 | 第87页 |
| ·Cloning Fragments of Tubulin from Genomic DNA | 第87-90页 |
| Chapter 4 Optimizing the Experiment Parameters | 第90-106页 |
| ·Real time PCR | 第90-92页 |
| ·Improved the first strand cDNA synthesis | 第92-100页 |
| ·Optimization of the Agrobacterium Concentration for Vacuum-Infiltration | 第100-104页 |
| ·Protein Standard Curve | 第104-106页 |
| Chapter 5 Transcription of GFP Using White Clover as a Host | 第106-119页 |
| ·Experimental Design | 第106-107页 |
| ·Characteristics of Plant Materials | 第107-109页 |
| ·Results of Quantifying the Expression of GFP in White Clover | 第109-116页 |
| ·Discussion | 第116-119页 |
| Chapter 6 Conclusions | 第119-135页 |
| Appendix | 第135-141页 |
| A Partial sequence of ?α-Tubulin | 第135页 |
| B Partial sequence of genomic Tubulins | 第135-137页 |
| C Clade of sequence alignment of Tubulins | 第137页 |
| D Figures of constructs | 第137-139页 |
| E Sequence of CaMV Double355 promoter, CaMV Terminator poly(A) and -90 core promotor | 第139-141页 |
| Acknowledgement | 第141-142页 |
| Publications | 第142页 |
