| ABBREVIATION LIST | 第13-15页 |
| 摘要 | 第15-19页 |
| ABSTRACT | 第19-23页 |
| CHAPTER-1 GENERAL INTRODUCTION | 第24-28页 |
| CHAPTER-ⅡREVIEW OF LITERATURE | 第28-48页 |
| 2.1 L-Arginine function, chemistry, source, metabolism, mode of action and its importance in ruminant animal health and production | 第28-33页 |
| 2.2 Mechanism of biosynthesis of Arg by NCG supplementation in ruminant animals | 第33-35页 |
| 2.3 Significance of NCG in dairy animal's production | 第35-46页 |
| 2.3.1 Lactation | 第35-37页 |
| 2.3.2 Nitrogen utilization | 第37-42页 |
| 2.3.3 Significance of N-carbamoylglutamate in reproduction | 第42-44页 |
| 2.3.4 Effect of NCG on growth and cell proliferation | 第44-46页 |
| Summary | 第46-48页 |
| CHAPTER-III Development of methods for the determination of Arginine and N-carbamoylglutamate in rumenfluid | 第48-64页 |
| 3.1. ABSTRACT | 第48-49页 |
| 3.2. INTRODUCTION | 第49-50页 |
| 3.3. MATERIALS AND METHODS | 第50-54页 |
| 3.3.1 Experimental Design | 第50页 |
| 3.3.2 Method of Arg and NCG determination in rumen fluid in invtro | 第50-51页 |
| 3.3.3 Chemical analytical procedures of Arg and NCG | 第51-53页 |
| 3.3.4 Setup of standard line for Arg and NCG determination | 第53-54页 |
| 3.3.5 Data Calculation and Statistical analysis | 第54页 |
| 3.4. RESULTS | 第54-59页 |
| 3.4.1 Effect of temperature and time on colour development and determination stability of Arg in rumen fluid | 第54-55页 |
| 3.4.2 Absorptive wavelength | 第55页 |
| 3.4.3 Determination of Arg concentration in rumen fluid | 第55-56页 |
| 3.4.4 Determination of NCG in rumen fluid | 第56-59页 |
| 3.5. DISCUSSION | 第59-64页 |
| 3.5.1 Effect of temperature and time on colour development and determination stability | 第59-60页 |
| 3.5.2 Absorptive wavelength | 第60页 |
| 3.5.3 Determination of Arg concentration in rumen fluid | 第60-61页 |
| 3.5.4 Determination of NCG in rumen fluid | 第61-64页 |
| CHAPTER-Ⅳ Evaluation of Arginine and N-carbamoylglutamate degradation in rumen and their effect onrumen fermentation in vitro | 第64-74页 |
| 4.1. ABSTRACT | 第64页 |
| 4.2. INTRODUCTION | 第64-66页 |
| 4.3. MATERIALS AND METHODS | 第66-69页 |
| 4.3.1 Experimental design | 第66页 |
| 4.3.2 In vitro incubation | 第66-67页 |
| 4.3.3 Measurement of in vitro fermentation variables | 第67-68页 |
| 4.3.4 Analysis of L-arg and NCG in rumen fluid | 第68页 |
| 4.3.5 Data calculation and statistical analysis | 第68-69页 |
| 4.4. RESULTS | 第69-70页 |
| 4.4.1 Degradation of L-arg and NCG in rumen fluid | 第69-70页 |
| 4.4.2 Effects on gas production and fermentation variables | 第70页 |
| 4.5. DISCUSSION | 第70-74页 |
| 4.5.1 Degradation of L-arg and NCG in rumen fluid | 第70-71页 |
| 4.5.2 Effects on gas production and fermentation variables | 第71-74页 |
| CHAPTER-Ⅴ The effects of N-carbamoylglutamate on milk production and nitrogen utilization of highyielding Chinese Holstein dairy cows | 第74-100页 |
| 5.1. ABSTRACT | 第74-75页 |
| 5.2. INTRODUCTION | 第75-76页 |
| 5.3. MATERIALS AND METHODS | 第76-83页 |
| 5.3.1 Animals, diet and experiment design | 第76-77页 |
| 5.3.2 Sampling, Measurement, and Analyses | 第77-80页 |
| 5.3.3 In Situ Digestion in the Rumen | 第80-81页 |
| 5.3.4 Estimation of MCP yield on PD excretion and Metabolizable Protein | 第81-82页 |
| 5.3.5 Statistical Analysis | 第82-83页 |
| 5.4. RESULTS | 第83-87页 |
| 5.4.1 DMI and Milk Performance | 第83-84页 |
| 5.4.2 Plasma Variables | 第84页 |
| 5.4.3 Plasma AA profile and N Utilization Efficiency | 第84-86页 |
| 5.4.4. Effect on MCP synthesis in rumen and metabolizable protein utilization | 第86-87页 |
| 5.5 DISCUSSION | 第87-100页 |
| 5.5.1 Effect on DMI and Milk | 第87-91页 |
| 5.5.2 Effect on plasma AA concentration | 第91-93页 |
| 5.5.3. Effect on plasma variables | 第93-94页 |
| 5.5.4 Effect on N utilization | 第94-96页 |
| 5.5.5. Effect on MCP synthesis in the rumen | 第96-97页 |
| 5.5.6. Effect on MP Utilization | 第97-100页 |
| CHAPTER-VI SUMMARY AND CONCLUSIONS | 第100-102页 |
| INNOVATION | 第102-104页 |
| REFERENCES | 第104-124页 |
| ACKNOWLEDGMENTS | 第124-126页 |
| CURRICULUM VITAE | 第126-127页 |
