| Acknowledgements | 第4-5页 |
| About Author | 第5-10页 |
| List of figures | 第10-15页 |
| Abbreviations: | 第15-16页 |
| <英文摘要> | 第16页 |
| Background | 第18-69页 |
| 0.1 Structure of Na,K-ATPase | 第18-20页 |
| 0.2 Function of Na,K-ATPase | 第20-21页 |
| 0.3. Role of Na,K-ATPase in kidney | 第21-25页 |
| 0.3.1. Distribution of Na,K-ATPase in kidney | 第22页 |
| 0.3.2 Intracellular location of Na,K-ATPase in kidney | 第22-23页 |
| 0.3.3. Function of Na,K-ATPase in kidney | 第23-25页 |
| 0.4. Regulation of Na,K-ATPase | 第25-38页 |
| 0.4.1 Short-term regulation of Na,K-ATPase activity | 第25-29页 |
| 0.4.2. Long-term regulation of Na,K-ATPase activity | 第29-31页 |
| 0.4.3. Signal pathway involved in the regulation of sodium pump | 第31-33页 |
| 0.4.4. Na,K-ATPase regulated by low K | 第33-38页 |
| 0.5. ROS | 第38-45页 |
| 0.5.1. Geneal concept of ROS | 第38-42页 |
| 0.5.2. ROS as second messenger | 第42-45页 |
| 0.6. Fe transport | 第45-49页 |
| <参考文献> | 第49-69页 |
| Introduction | 第69-73页 |
| Chapter 1 ROS was involved in the up-regulation of Na,K-ATPase induced by low K | 第73-100页 |
| 1.1 METHODS AND MATERIALS | 第74-79页 |
| 1.2. Low K+ stimulates the function of the Na,K-ATPase. | 第79-86页 |
| 1.3. Stimulation of the Na,K-ATPase by low K+ requires ROS | 第86-92页 |
| 1.4. Effect of low K on the alpha promoter activity of Na,K-ATPase | 第92-96页 |
| 1.5. Summary | 第96-97页 |
| <参考文献> | 第97-100页 |
| Chapter 2 Hydrogen peroxide was specifically involved in the up-regulation of Na,K-ATPase induced by low K | 第100-119页 |
| 2.1. Introduction: | 第101-102页 |
| 2.2. Methods and Materials: | 第102-109页 |
| 2.3. PCR reaction | 第109-110页 |
| 2.4. Identification of recombinant DNA by restriction enzyme digestion | 第110页 |
| 2.5. Sequence confirmation of recombinant DNA with 96 base pairs of alpha subunit of Na,K-ATPase. | 第110-111页 |
| 2.6. Screen of MDCK cells transfected with human catalase | 第111页 |
| 2.7. Effects of tiron, tempo and catalase on the activity of Na,K-ATPase | 第111-113页 |
| 2.8. Effects of catalase, tempo and catalase on low K+-induced | 第113-115页 |
| 2.9. Effect of catalase, tempo and tiron on low K+-induced | 第115-116页 |
| 2.10. summary | 第116-117页 |
| <参考文献> | 第117-119页 |
| Chapter 3 Up-regulation of Na,K-ATPase was dependent on transferrin | 第119-141页 |
| 3.1. Introduction | 第120-121页 |
| 3.2. Methods and materials | 第121-123页 |
| 3.3. RESULTS | 第123-137页 |
| 3.4. Summary | 第137-138页 |
| <参考文献> | 第138-141页 |
| Chapter 4 Mechanism of up-regulation of Na,K-ATPase induced by low K | 第141-167页 |
| 4.1. Introduction | 第142-143页 |
| 4.2. Methods and Materials | 第143-146页 |
| 4.3. The increase in ROS production is necessary to stimulate the promoter activity, but not sufficient to increase protein abundance and surface binding sites of the Na,K-ATPase. | 第146-152页 |
| 4.4. Transcriptional regulation is necessary for the up-regulation of Na,K-ATPase induced by low K | 第152-154页 |
| 4.5. The continuous presence of low K+ is required for low K+ to increase the function of the Na,K-ATPase. | 第154-159页 |
| 4.6. Sp1, rather than Nf-kB was involved in the up-regulation of alpha subunit promoter activity of Na,K-Atpase induced by low K | 第159-164页 |
| 4.7. Summary | 第164页 |
| <参考文献> | 第164-167页 |
| Discussion | 第167-184页 |
| <参考文献> | 第176-184页 |
| <中文摘要> | 第184-198页 |
