| Abstract | 第8-10页 |
| 摘要 | 第11-13页 |
| List of abbreviations | 第13-15页 |
| 1 General Introduction | 第15-33页 |
| 1.1 Morphology and Flower development | 第15-17页 |
| 1.2 History and Cultivation area | 第17-19页 |
| 1.3 Plant grandular trichome:specialized metabolites factories | 第19-20页 |
| 1.4 Chemistry of pyrethrins | 第20-21页 |
| 1.5 Role of pyrethrins as natural insecticide | 第21-22页 |
| 1.6 Pyrethrins Biosynthesis | 第22-24页 |
| 1.7 Trichome specific promoter | 第24-25页 |
| 1.8 Metabolic engineering of plant secondary metabolites | 第25-26页 |
| 1.9 Molecular evolution and phylogeny of the nuclear gene | 第26-28页 |
| 1.10 Objective of the thesis | 第28-33页 |
| 2 Molecular Clining and Characterization of the Trichome Specific ChrysanthemylDiphosphate Synthase Promoter from Tanacetum cinerariifolium | 第33-47页 |
| 2.1 Abstract | 第33页 |
| 2.2 Introduction | 第33-36页 |
| 2.3 Materials and methods | 第36-38页 |
| 2.3.1 Plant materials | 第36页 |
| 2.3.2 Promoter cloning | 第36-37页 |
| 2.3.3 DNA sequence analysis | 第37页 |
| 2.3.4 Vector construction | 第37-38页 |
| 2.3.5 Plant transformation | 第38页 |
| 2.3.6 Gus assay | 第38页 |
| 2.4 Results | 第38-40页 |
| 2.4.1 Characterization of the promoter DNA sequence | 第38-40页 |
| 2.4.2 Tissue specificity of the TcCDS promoter in tobacco | 第40页 |
| 2.5 Discussion | 第40-47页 |
| 3 Overexpression of the genes Chrysanthemyl Diphosphate Synthase (TcCDS) increasedPyrethrins content in Tanacetum cinerariifolium | 第47-65页 |
| 3.1 Abstract | 第47页 |
| 3.2 Introduction | 第47-50页 |
| 3.3 Material and Methods | 第50-56页 |
| 3.3.1 Plant materials | 第50-51页 |
| 3.3.2 Construction of pBINPLUS-CDS and transformation of Agrobacterium tumefaciens strain AGL-0 | 第51页 |
| 3.3.3 Genetic transformation of T cinerariifolium | 第51-52页 |
| 3.3.4 Selection and regeneration of transformants | 第52-53页 |
| 3.3.5 DNA isolation and PCR analysis | 第53-54页 |
| 3.3.6 Phenotype analysis of transgenic T cinerariifolium plants | 第54页 |
| 3.3.7 RNA isolation and real-time qPCR analysis | 第54-55页 |
| 3.3.8 Pyrethrins Extraction methods for HPLC Analysis | 第55页 |
| 3.3.9 Pyrethrins evaluation by High-performance liquid chromatography (HPLC) | 第55-56页 |
| 3.3.10 Statistical analysis | 第56页 |
| 3.4 Results | 第56-61页 |
| 3.5 Discussion | 第61-65页 |
| 4 Molecular evolution and phylogenetic utility of the chrysanthemyl diphosphatesynthase (TcCDS) gene,resolving relationship within Chrysanthemum | 第65-83页 |
| 4.1 Abstrace | 第65-66页 |
| 4.2 Introduction | 第66-69页 |
| 4.3 Materials and methods | 第69-72页 |
| 4.3.1 Plant material and genomic DNA isolation | 第69-70页 |
| 4.3.2 Nested PCR amplification and purification | 第70-71页 |
| 4.3.3 Cloning and sequencing | 第71页 |
| 4.3.4 DNA sequenco analysis | 第71-72页 |
| 4 Result | 第72-77页 |
| 4.1 Gene characterization and genetic analysis | 第72页 |
| 4.2 Number of nueleotide substitutions per site | 第72-73页 |
| 4.3 Synonymous and nonsynonymous substitutions | 第73-75页 |
| 4.4 Phylogenetic analysis | 第75-77页 |
| 4.5 Discussion | 第77-83页 |
| 5 General Conclusion and Prospects | 第83-88页 |
| 5.1 Molecular Cloning and Characterization of the Trichome Speoitic Chrysanthemyl Diphosphate Promoter from Tanacetum cinerariifolium | 第84页 |
| 5.2 Overexpression of the genes Chrysanthemyl Diphosphate Synthase(TcCDS)increasedpyrethrins content in Tanacetum cinerariifolium | 第84-85页 |
| 5.3 The molecular evolution and phylogenetic utility of the chrysanthemyl diphosphatesynthase (CDS) gene in Chrysanthemum | 第85-88页 |
| References | 第88-101页 |
| Acknowledgements | 第101-103页 |
| Resume | 第103-104页 |
| List of Publications | 第104页 |
