| 摘要 | 第1-8页 |
| Abstract | 第8-13页 |
| ABREVIATION | 第13-15页 |
| DEDICATION | 第15-16页 |
| CHAPTER ONE Introduction | 第16-34页 |
| ·Cotton (Gossypium spp.) | 第16-17页 |
| ·Drought | 第17-19页 |
| ·Low Temperature Stress | 第19-22页 |
| ·Increased chlorophyll accumulation and reduced sensitivity of Photosynthesis | 第20-21页 |
| ·Mechanisms for understanding tolerance to cold injury | 第21-22页 |
| ·Cold tolerance using transgenic approaches | 第22页 |
| ·Plant SnRK2 | 第22-27页 |
| ·SnRK1 subfamily | 第24-25页 |
| ·SnRK2 subfamily | 第25-26页 |
| ·Structure of SnRK2 | 第26页 |
| ·SnRK3 Subfamily | 第26-27页 |
| ·Gene cloning | 第27-31页 |
| ·Agrobacterium-mediated transformation | 第28-29页 |
| ·Virulence Gene Expression and Incorporating Genes into Plants by Using Agrobacterium | 第29-31页 |
| ·Virus-induced gene silencing (VIGS) | 第31-32页 |
| ·Hypothesis | 第32-33页 |
| ·Objectives of the research | 第33-34页 |
| CHAPTER TWO Materials and method | 第34-43页 |
| ·Cloning of GhSnRK2 gene | 第34页 |
| ·Localization of GhSnRK2-GFP fusion protein | 第34-35页 |
| ·Plant transformation and screening of transgenic plants | 第35页 |
| ·Plant material and growth conditions | 第35-36页 |
| ·Drought tolerance assay | 第36页 |
| ·Freezing and cold Stress Treatment | 第36-37页 |
| ·Transpirational water loss assay | 第37页 |
| ·Relative water content | 第37页 |
| ·Determination of proline | 第37-38页 |
| ·ChlorophyⅡ content Assay | 第38页 |
| ·Measurement of stomatal closure in response to ABA treatment | 第38页 |
| ·Seedlings growth in response to ABA and NaCl treatment | 第38-39页 |
| ·Germination Assay | 第39页 |
| ·Silencing construct development/VIGS protocol and cotton transformation | 第39-40页 |
| ·RT-QPCR | 第40-42页 |
| ·Experimental design and statistical analysis | 第42-43页 |
| CHAPTER THREE Result | 第43-82页 |
| ·Phylogenetic analysis and multiple sequence alignment of GhSnRK2 and closely related SnRK2s from other plant species | 第43-46页 |
| ·Conserved domains of GhSnRK2 protein sequence | 第46页 |
| ·Chromosome location of GhSnRK2 gene | 第46-47页 |
| ·Subcellular localization of GhSnRK2-GFP fusion protein | 第47-50页 |
| ·GhSnRK2 gene in transgenic Arabidopsis | 第50-51页 |
| ·Expression pattern of GhSnRK2 gene in response to polyethylene glycol treatment and Tissue specificity | 第51-52页 |
| ·Construction of plant transformation vector | 第52-53页 |
| ·Expression pattern of GhSnRK2 gene in transgenic line | 第53-55页 |
| ·Overexpression of Ghsnrk2 in transgenic plant improves plant tolerance to Drought and cold stress and biomass accumulation # | 第55-60页 |
| ·GhSnRK2 in transgenic plant improves transpiration water loss, Relative water content and reduce stomata aperture | 第60-63页 |
| ·Overexpression of GhSnRK2 in transgenic plant improves the Proline and ChlorophyⅡ content | 第63-65页 |
| ·Overexpression of GhSnRK2 in transgenic plant enhance seedlings growth in response to NaCl treatment | 第65-67页 |
| ·Seedlings growth in response to ABA treatment was enhance in plant Overexpressing GhSnRK2 | 第67-69页 |
| ·GhSnRK2 enhanced NaCl and ABA-mediated inhibition of seed germination | 第69-71页 |
| ·Expression analysis of stress-responsive genes | 第71-73页 |
| ·Virus induced gene silencing construct and cotton transformation | 第73-75页 |
| ·Virus induced gene silencing for functional analysis of GhSnRK2 in Gossypium hirsutum | 第75-79页 |
| ·Water loss, relative water content and effect salt stress on GhSnRK2 silenced plant | 第79-82页 |
| CHAPTER FOUR Discussion | 第82-90页 |
| CHAPTER FIVE Conclusion | 第90-92页 |
| Reference | 第92-119页 |
| Acknowledgement | 第119-121页 |
| Resume | 第121-122页 |
| Appendix | 第122页 |
