Abbreviations | 第1-8页 |
中文摘要 | 第8-9页 |
Abstract | 第9-11页 |
Chapter 1:Introduction | 第11-37页 |
Overview | 第11-12页 |
·The floral structure of the model dicot Arabidopsis thaliana and the model monocot Oryza sativa | 第12-15页 |
·ABC model for flower development | 第15-23页 |
·Molecular regulation of rice floral organ identity | 第23-32页 |
·Recent progress on rice spikelet mutants | 第32-37页 |
Chapter 2:Materials and Methods | 第37-43页 |
·Plant materials | 第37页 |
·Observation of major agronomic traits and flower phenotypes of Oasf mutants | 第37页 |
·Genetic analysis and the mapping population of rice | 第37页 |
·SSR markers and other primers | 第37-38页 |
·DNA extraction | 第38页 |
·PCR analysis | 第38-39页 |
·The PCR amplifications | 第38-39页 |
·Molecular marker analysis | 第39页 |
·Gene mapping of the OsAF gene controlling floral organ development in rice | 第39-40页 |
·Fine mapping of the OsAF gene controlling floral organ development in rice | 第40-41页 |
·Candidate gene prediction and sequencing | 第41页 |
·Phylogenetic analysis and multiple sequence alignments of the OsAF gene | 第41-43页 |
Chapter 3:Results and Analysis | 第43-55页 |
·Phenotypic characterization of the Osaf mutant | 第43-46页 |
·Genetic analysis of the gene for the Osaf mutant | 第46-47页 |
·Gene mapping of the OSAF gene | 第47-52页 |
·DNA sequence analysis and phylogenetic analysis of the OsAF Gene | 第52-55页 |
Chapter 4:Discussions | 第55-59页 |
·OsAF is a key regulator of rice floral organ identity | 第55-56页 |
·OsAF encodes the class III triacylglycerol (TAG) lipase | 第56-59页 |
References | 第59-71页 |
Appendix | 第71-73页 |
Acknowledgement | 第73页 |