| DEDICATION | 第6-7页 |
| 摘要 | 第7-8页 |
| abstract | 第8页 |
| Abbreviations | 第15-16页 |
| CHAPTERⅠ INTRODUCTION | 第16-27页 |
| 1.1 Plant defence responses | 第16-17页 |
| 1.2 The systemic resistance of plants | 第17-22页 |
| 1.2.1 Plant systemic resistance types | 第17-18页 |
| 1.2.2 Plant systemic resistance pathways | 第18-20页 |
| 1.2.3 Reactive oxygen species and cell death | 第20-21页 |
| 1.2.4 Bioassay of elicitor protein-induced disease resistance | 第21-22页 |
| 1.3 Brevibacillus laterosporus in biological control | 第22-24页 |
| 1.3.1 Brevibacillus laterosporus | 第22页 |
| 1.3.2 The insecticidal functions | 第22页 |
| 1.3.3 The bactericidal function | 第22-23页 |
| 1.3.4 The induced systemic resistance | 第23页 |
| 1.3.5 Application status and problems | 第23-24页 |
| 1.3.6 Induction of plant defence response elicitor | 第24页 |
| 1.4 Protein elicitor | 第24-25页 |
| 1.4.1 Hypersensitive protein(Harpin) | 第24页 |
| 1.4.2 Secreted protein(eliciting) | 第24-25页 |
| 1.5 Biocontrol protein elicitor | 第25-26页 |
| 1.5.1 Fungal biocontrol protein elicitor | 第25页 |
| 1.5.2 Bacterial biocontrol protein elicitor | 第25-26页 |
| 1.5.3 Bacterial systemic resistant elicitor | 第26页 |
| 1.6 Purpose and significance | 第26-27页 |
| CHAPTERⅡ Isolation,purification& characterization of the protein from B.laterosporus | 第27-37页 |
| 2.1 Introduction | 第27页 |
| 2.2 Materials and Methods | 第27-31页 |
| 2.2.1 Media | 第27页 |
| 2.2.2 Screening of Brevibacillus laterosporus activity against fungus | 第27-28页 |
| 2.2.3 Protein elicitor assays | 第28页 |
| 2.2.4 Preparation culture of Brevibacillus laterosporus and A60 broth | 第28页 |
| 2.2.5 Preparation of crude protein and detection of biological activity | 第28-29页 |
| 2.2.6 Isolation and purification of protein elicitors | 第29页 |
| 2.2.7 Purification of single protein elicitor by HPLC | 第29-30页 |
| 2.2.8 Molecular mass determination and amino acid sequence analysis | 第30页 |
| 2.2.9 Elicitor bioassay | 第30-31页 |
| 2.3 Results | 第31-35页 |
| 2.3.1 Screening of bacteria showing protein elicitor activity against fungal disease. | 第31页 |
| 2.3.2 Isolation and purification of crude protein from Brevibacillus laterosporus | 第31-32页 |
| 2.3.3 High-performance liquid chromatography(HPLC) | 第32-33页 |
| 2.3.4 Electrophoresis Tricine-SDS-PAGE | 第33页 |
| 2.3.5 Molecular mass determination and amino acid sequence analysis | 第33-34页 |
| 2.3.6 Elicitor bioassay | 第34-35页 |
| 2.4 Discussion | 第35-37页 |
| ChapterⅢ Gene identification and molecular cloning | 第37-49页 |
| 3.1 Introduction | 第37-38页 |
| 3.2 Materials and Methods | 第38-40页 |
| 3.2.1 Identification of pebl2 gene sequence by PCR | 第38页 |
| 3.2.2 pebl2 gene cloned with the pMD18-T simple vector | 第38-40页 |
| 3.2.3 The protein expression by vector pET28-A | 第40页 |
| 3.3 Results | 第40-47页 |
| 3.3.1 Gene cloning | 第40-46页 |
| 3.3.2 Hypersensitive response induced by the protein elicitor | 第46页 |
| 3.3.3 peBL2 gene sequence and heterologous expression | 第46-47页 |
| 3.4 Discussion | 第47-49页 |
| CHAPTERⅣ Purification and expression of the protein elicitor PeBL | 第49-58页 |
| 4.1 Introduction | 第49页 |
| 4.2 Materials and Methods | 第49-52页 |
| 4.2.1 Protein expression BL-21(DE3)to IPTG | 第49-50页 |
| 4.2.2 PeBL2 expression method | 第50-51页 |
| 4.2.3 Characteristics of protein elicitor | 第51页 |
| 4.2.4 Detection reactive oxygen species(ROS) | 第51页 |
| 4.2.5 Bioassay for PeBL2-induced disease resistance in tobacco leaf | 第51页 |
| 4.2.6 Protein assay | 第51-52页 |
| 4.3 Results | 第52-55页 |
| 4.3.1 Expression and purification of the protein | 第52页 |
| 4.3.2 The biological activity of the expressed protein | 第52-53页 |
| 4.3.3 PeBL2 induced plant systemic resistance against Botrytis cinerea | 第53-54页 |
| 4.3.4 Detection of reactive oxygen species(ROS) | 第54-55页 |
| 4.4 Discussion | 第55-58页 |
| Conclusion | 第58-59页 |
| References | 第59-71页 |
| ACKNOWLEDGEMENTS | 第71-72页 |
| C.V GHULAM HUSSAIN JATOI | 第72-76页 |
