| ABSTRACT | 第11-13页 |
| 摘要 | 第14-16页 |
| ABBREVIATION | 第16-18页 |
| INTRODUCTION | 第18-23页 |
| RESEARCH AIMS | 第20页 |
| REFERENCES | 第20-23页 |
| PART ONE | 第23-55页 |
| CHAPTER 1 LITERATURE REVIEW THE ROLE OF THYROID HORMONES IN OVARIAN FOLLICULAR DEVELOPMENT ANDFUNCTION | 第23-43页 |
| 1.1 THE ANATOMY AND FUNCTIONS OF THE THYROID GLAND | 第23-25页 |
| 1.2 REGULATION OF OF T3 AND T4 | 第25-26页 |
| 1.3 THE SYNTHESIS OF THYROID HORMONES | 第26-27页 |
| 1.4 THE MECHANISM OF THYROID HORMONES | 第27-28页 |
| 1.5 THYROID HORMONES TRANSPORTATION | 第28-30页 |
| 1.6 THE HYPOTHALAMIC PITUITARY THYROID AXIS(HPT) AND FEMALE REPRODUCTIVES SYSTEM | 第30-31页 |
| 1.7 THE EFFECT OF THYROID HORMONES IN NORMAL OVARIAN FOLLICLES DEVELOPMENT | 第31-33页 |
| 1.8 HYPERTHYROIDISM AND INFERTILITY | 第33页 |
| 1.9 HYPOTHYROIDISM AND INFERTILITY | 第33-34页 |
| 1.10 EFFECT OF THYROID HORMONES IN OVARIAN FOLLICULAR DEVELOPMENT AND FUNCTION INHYPER AND HYPOTHYROID NEONATALAND IMMATURE RATS | 第34-35页 |
| 1.11 EFFECT OF THYROID HORMONES ON THE OVARIAN FOLLICULAR DEVELOPMENT AND FUNCTION INHYPER AND HYPOTHYROID ADULT RATS | 第35-36页 |
| REFERENCES | 第36-43页 |
| CHAPTER 2 THE ROLES OF NITRIC OXIDE(NO) OVARIAN DEVELOPMENT AND FUNCTION INHYPER AND HYPOTHYROID RATS | 第43-55页 |
| 2.1 GENERAL CHARACTERISTICS OF NOS | 第43-45页 |
| 2.2 THE NOS MOLECULAR STRUCTURE AND BIOSYNTHESIS | 第45-46页 |
| 2.3 NOS GENE CATALYTIC MECHANISM AND FUNCTION | 第46-47页 |
| 2.4 NITRIC OXIDE REGULATION AND INHIBITION | 第47-48页 |
| 2.5 NITRIC OXIDE AND OVARIAN FUNCTION | 第48-49页 |
| 2.6 EFFECT OF NITRIC OXIDE ACTIVITY IN OVARIAN FOLLICULAR DEVELOPMENT AND FUNCTION INHYPER AND HYPOTHYROID RATS | 第49-50页 |
| REFERENCES | 第50-55页 |
| PART TWO EXPERIMENT STUDY'S | 第55-129页 |
| CHAPTER 3 ROLES OF THYROID HORMONES IN FOLLICULAR DEVELOPMENT IN THE OVARY OF NEONATAL AND IMMATURE RATS | 第55-79页 |
| 3.1 INTRODUCTION | 第56-58页 |
| 3.2 MATERIALS AND METHODS | 第58-61页 |
| 3.2.1 Animals | 第58页 |
| 3.2.2 Experimental design | 第58页 |
| 3.2.3 Tissue preparation | 第58-59页 |
| 3.2.4 Radioimmunoassay(RIA) for triiodothyronine(T3),thyroxine(T4)and 17β-estradiol(E2) | 第59页 |
| 3.2.5 Histologic examination | 第59页 |
| 3.2.6 Immunohistochemistry | 第59-60页 |
| 3.2.7 Measurement of NOS activity | 第60页 |
| 3.2.8 Statistical analysis | 第60-61页 |
| 3.3 RESULTS | 第61-70页 |
| 3.3.1 Body weights of control,and induced hyperthyroid and hypothyroid rats | 第61页 |
| 3.3.2 Serum hormone concentrations in control,hyper and hypo rats | 第61-62页 |
| 3.3.3 Ovarian histologic and morphometric analyses | 第62-64页 |
| 3.3.4 Immunohistochemical staining of TRα1 and NOS isoforms in ovaries of neonatal rats | 第64-70页 |
| 3.3.5 Measurement of NOS activity | 第70页 |
| 3.4 DISCUSSION | 第70-74页 |
| REFERENCES | 第74-79页 |
| CHAPTER 4 NITRIC OXIDE AND THYROID HORMONE RECEPTOR ALPHA1CONTRIBUTE TO OVARIAN FOLLICULAR DEVELOPMENT IN IMMATURE HYPER-AND HYPO-THYROIDRATS | 第79-99页 |
| 4.1 INTRODUCTION | 第80-81页 |
| 4.2 MATERIALS AND METHODS | 第81-85页 |
| 4.2.1 Animals and experimental design | 第81-82页 |
| 4.2.2 Radioimmunoassay of hormones | 第82页 |
| 4.2.3 Protein homogenate preparation and hormone management | 第82页 |
| 4.2.4 Histologic examination and follicle counts | 第82-83页 |
| 4.2.5 Immunohistochemical Analysis | 第83-84页 |
| 4.2.6 Measurement of ovarian contents of NO | 第84页 |
| 4.2.7 Measurement of ovarian NOS activity | 第84页 |
| 4.2.8 Statistical analysis | 第84-85页 |
| 4.3 RESULTS | 第85-94页 |
| 4.3.1 Serum hormone measurements | 第85-86页 |
| 4.3.2 Histologic examination of follicular development | 第86-87页 |
| 4.3.3 Immunohistochemical localization of NOS isoforms and TRα1 | 第87-92页 |
| 4.3.4 Measurement of ovarian contents of NO | 第92-93页 |
| 4.3.5 Measurement of ovarian activity of NOS | 第93-94页 |
| 4.4 DISSCUSSION | 第94-95页 |
| REFERENCES | 第95-99页 |
| CHAPTER 5 EFFECTS OF THYROID HORMONES IN PUBERTY ONSET AND ESTROUS CYCLICITY OFYOUNG ADULT RATS | 第99-129页 |
| 5.1 INTRODUCTION | 第100-101页 |
| 5.2 MATERIALS AND METHODS | 第101-106页 |
| 5.2.1 Ethics statement | 第101-102页 |
| 5.2.2 Reagents | 第102页 |
| 5.2.3 Animals and condition | 第102页 |
| 5.2.4 Experimental design | 第102-103页 |
| 5.2.5 Tissue preparation | 第103页 |
| 5.2.6 Observation of vaginal opening days and estrous cyclicity | 第103-104页 |
| 5.2.7 Determination of serum concentrations of hormones | 第104页 |
| 5.2.8 Histology and follicle counting | 第104页 |
| 5.2.9 Protein extraction and western blot analysis | 第104-105页 |
| 5.2.10 Immunohistochemistry | 第105-106页 |
| 5.2.11 Statistical analysis | 第106页 |
| 5.3 RESULTS | 第106-119页 |
| 5.3.1 Body and ovarian weights of control and induced hyperthyroid and hypothyroid rats | 第106-107页 |
| 5.3.2 Vaginal opening(VO)days and estrous cycles in control,hyperthyroid and hypothyroidrats | 第107-111页 |
| 5.3.3 Serum concentrations of hormones in control,hyperthyroid and hypothyroid rats | 第111-113页 |
| 5.3.4 Ovarian histologic and morphometric examination | 第113-114页 |
| 5.3.5 TRal involvement in the process of follicular development | 第114-115页 |
| 5.3.6 Immunohistochemical detection of TRα in the ovaries of adult rats | 第115-119页 |
| 5.4 DISSCUSSION | 第119-123页 |
| REFERENCE | 第123-129页 |
| CONCLUSIONS | 第129-131页 |
| INNOVATION | 第131-133页 |
| FUTURE RESEARCH OPENINGS | 第133-135页 |
| LIST OF PUBLISHED,SUBMITTED AND UNDER PREPARATION PAPERS | 第135-137页 |
| ACKNOWLEDGEMENTS | 第137-139页 |
| APPENDIX GENERAL MATERIALS AND METHODS | 第139-160页 |
| 1 ANIMALS AND EXPERIMENT DESIGN | 第139-140页 |
| 2 HORMONE RADIOIMMUNOASSAY(RIA) OF T3 AND T4 | 第140-142页 |
| 3 HORMONE RADIOIMMUNOASSAY(RIA) OF FSH AND LH | 第142-144页 |
| 4 RATS OVARIAN TISSUE SAMPLES EMBEDDED IN PARAFFIN PROCESSING | 第144-146页 |
| 5 HEMATOXYLIN AND EOSIN(HE) SLIDES STAINING | 第146-148页 |
| 6 IMMUNOHISTOCHEMISTRY | 第148-151页 |
| 7 WESTERN BLOTTING ANALYSIS | 第151-160页 |
| References | 第160页 |
