| 摘要 | 第1-6页 |
| Abstract | 第6-9页 |
| Table of contents | 第9-11页 |
| Chapter 1 General introduction | 第11-23页 |
| 1 Cyanobacterial blooms and cyanotoxins | 第11-12页 |
| 2 Structure and acute toxicity of microcystins | 第12-13页 |
| 3 Microcystin detoxification-related liver genes | 第13-22页 |
| ·Soluble glutathione S-transferase | 第14-18页 |
| ·Crystal structures of GSTs | 第15-16页 |
| ·Regulation of GST gene expression | 第16-18页 |
| ·Uncoupling protein 2 | 第18-22页 |
| ·The uncoupling protein family | 第18-19页 |
| ·Gene locus and structure features | 第19-20页 |
| ·Regulatory expression and function of UCP2 | 第20-22页 |
| 4 Objectives of current study | 第22-23页 |
| Chapter 2 Molecular cloning and characterization of alpha-class glutathione S-transferase gene from the liver of silver carp, bighead carp and other major Chinese freshwater fishes | 第23-44页 |
| 1 Introduction | 第23-25页 |
| 2 Materials and methods | 第25-30页 |
| 3 Results | 第30-40页 |
| ·Cloning and sequence analysis of the silver carp and bighead carp GST cDNAs | 第30页 |
| ·Isolation and characterization of silver carp GST gene 5'-flanking region | 第30-32页 |
| ·Cloning and alignment of the freshwater fish GST gene | 第32-37页 |
| ·Secondary structure prediction for silver carp GST | 第37-38页 |
| ·Liver GST mRNA expression in six freshwater fishes | 第38-40页 |
| 4 Discussion | 第40-44页 |
| Chapter 3 Structural conservation and food habit-related liver expression of uncoupling protein 2 gene in five major Chinese carps | 第44-58页 |
| 1 Introduction | 第44-45页 |
| 2 Materials and Methods | 第45-49页 |
| 3 Results | 第49-55页 |
| 4 Discussion | 第55-58页 |
| References | 第58-78页 |
| Appendices | 第78-83页 |
| Acknowledgements | 第83-84页 |
| Papers during the course | 第84-85页 |
