| 摘要 | 第6-8页 |
| abstract | 第8-9页 |
| LIST OF ABBRIVATION | 第17-18页 |
| CHAPTER 1 INTRODUCTION | 第18-39页 |
| 1.1 EARLY LEAF DEVELOPMENTAL EVENTS | 第18-21页 |
| 1.1.1 Hormone Signaling and Transcription Factor Networks | 第18-19页 |
| 1.1.2 Definition of the Dorso-Ventral Axis of the Leaf | 第19-20页 |
| 1.1.3 Control of leaf growth by Cell expansion | 第20-21页 |
| 1.2 FACTORS EFFECTING LEAF SIZE | 第21页 |
| 1.3 LEAF SENESCENCE | 第21-22页 |
| 1.4 PROGRAMMED CELL DEATH DURING LEAF SENESCENCE | 第22页 |
| 1.5 TRANSCRIPTIONAL REGULATION OF PLANT LEAF SENESCENCE | 第22-23页 |
| 1.6 HORMONE SIGNALING DURING LEAF SENESCENCE | 第23页 |
| 1.7 ROS AND LEAF SENESCENCE | 第23-24页 |
| 1.8 ROLE OF BHLH TRANSCRIPTION FACTORS IN LEAF SENESCENCE | 第24-25页 |
| 1.9 STRUCTURE OF MAIZE KERNEL | 第25-26页 |
| 1.9.1 Embryo | 第25页 |
| 1.9.2 Coleorhiza | 第25-26页 |
| 1.10 MAIZE KERNEL DEVELOPMENTAL STAGES | 第26-29页 |
| 1.11 CORN GRAIN FILL STAGES | 第29-30页 |
| 1.12 FACTORS AFFECTING ON SEED SIZE | 第30-37页 |
| 1.12.1 Transcription factors in early maize kernel development stages | 第35-36页 |
| 1.12.2 The bHLH Transcription factor Family and their role in maize kernel development | 第36-37页 |
| 1.13 SCOPE AND OUTLINE OF THIS THESIS | 第37-39页 |
| CHAPTER 2 MATERIALS AND METHODS | 第39-58页 |
| 2.1 MATERIALS | 第39-41页 |
| 2.1.1 Plant materials | 第39-40页 |
| 2.1.2 Bacterial and yeast strains | 第40页 |
| 2.1.3 Constructs used for this thesis(Stored in our Lab) | 第40页 |
| 2.1.4 Instruments used for this study | 第40页 |
| 2.1.5 Biochemicals | 第40-41页 |
| 2.2 MEDIA AND SOLUTIONS | 第41-43页 |
| 2.3 BUFFERS AND REAGENTS | 第43-44页 |
| 2.4 PROTOPLAST TRANSFORMATION ASSAY PROTOCOL | 第44页 |
| 2.5 YEAST ONE-HYBRID PROTOCOL | 第44-46页 |
| 2.6 CLONING OF GENE AND VECTOR CONSTRUCTION | 第46-48页 |
| 2.7 KERNEL PHENOTYPING | 第48页 |
| 2.8 GRAIN FILLING RATE MEASUREMENT | 第48页 |
| 2.9 GUS STAINING METHOD | 第48-49页 |
| 2.10 GUS FLUORESCENT QUANTIFICATION | 第49页 |
| 2.11 PARAFFIN TECHNIQUE FOR HISTOLOGICAL STUDY OF THE GUS STAINED TISSUES | 第49-50页 |
| 2.12 SAMPLE PREPARATION FOR RNA-SEQ | 第50页 |
| 2.13 TRANSCRIPTOME SEQUENCING AND ANALYSIS | 第50-51页 |
| 2.14 SDS-PAGE | 第51页 |
| 2.15 PROTOCOL | 第51-52页 |
| 2.16 WESTERN BLOT | 第52-53页 |
| 2.17 CHIP-SEQ | 第53-57页 |
| 2.18 LEAF CHLOROPHYLL ANALYSIS | 第57页 |
| 2.19 3,3-DIAMINOBENZIDINE(DAB),NITROBLUE TETRAZOLIUM CHLORIDE(NBT)STAINING | 第57-58页 |
| CHAPTER 3 RESULTS | 第58-96页 |
| 3.1 IDENTIFICATION AND CHARACTERIZATION OF ABP7-LIKE | 第58-64页 |
| 3.1.1 Identification of ABP7-Like | 第58-59页 |
| 3.1.2 Transcriptional activation activity analysis of ABP7-Like | 第59-62页 |
| 3.1.3 DNA binding activity analysis of ABP7-Like | 第62-64页 |
| 3.2 EXPRESSION PATTERN ANALYSIS OF ABP7-LIKE DURING THE VEGETATIVE AND REPRODUCTIVE STAGES | 第64-68页 |
| 3.3 FUNCTIONAL AND MOLECULAR MECHANISM ANALYSIS OF ABP7-LIKE TRANSCRIPTION FACTOR IN LEAF DEVELOPMENT AT VEGETATIVE STAGE | 第68-79页 |
| 3.3.1 Phenotypes of the transgenic plants during vegetative growth | 第70-73页 |
| 3.3.2 Changes in ROS accumulation and chlorophyll content in the transgenic plants during vegetative growth | 第73-74页 |
| 3.3.3 Transcriptome analysis of ABP7-Like-OE,ABP7-Like-RNAi,ABP7-Like-SRDX and ABP7-Like-VP64 leaves | 第74-79页 |
| 3.4 FUNCTIONAL AND MOLECULAR MECHANISM ANALYSIS OF ABP7-LIKE TRANSCRIPTION FACTOR AT REPRODUCTIVE STAGE | 第79-96页 |
| 3.4.1 The effect of ABP7-Like on kernel traits | 第79-83页 |
| 3.4.2 The effect of ABP7-Like on grain filling rate | 第83-84页 |
| 3.4.3 Transcriptome analysis of ABP7-Like-OE,ABP7-Like-RNAi,ABP7-Like-SRDX kernels | 第84-89页 |
| 3.4.4 ChIP-Seq Analysis of genome-wide DNA-binding sites of ABP7-Like | 第89-92页 |
| 3.4.5 Validation of potential direct target genes of ABP7-Like | 第92-96页 |
| CHAPTER 4 DISCUSSION | 第96-103页 |
| CHAPTER 5 CONCLUSION AND PROSPECT | 第103-105页 |
| 5.1 CONCLUSION | 第103页 |
| 5.2 PROSPECT | 第103-105页 |
| REFERENCES | 第105-120页 |
| APPENDICES | 第120-130页 |
| ACKNOWLEDGEMENT | 第130-131页 |
| RESUME | 第131页 |
