| 摘要 | 第7-9页 |
| Abstract | 第9-10页 |
| Abbreviation | 第11-12页 |
| CHAPTER Ⅰ Introduction | 第12-26页 |
| 1.1 Terpenoids: An overview | 第12-13页 |
| 1.2 Pathways mapping and functional genomics | 第13-16页 |
| 1.3 Biosynthesis of IPP and DMAPP | 第16-18页 |
| 1.4 Discovery of genes, enzymes, and intermediates involved in MVA (Ac-MVA) pathway forbiosynthesis of terpenoids | 第18-20页 |
| 1.5 Discovery of genes, enzymes, and intermediates involved in non-MVA (MEP) pathway forbiosynthesis of terpenoids | 第20-22页 |
| 1.6 Terpene synthases (TPS) | 第22-23页 |
| 1.7 Garden sage (Salvia oicinalis L.) and Blue Anise Sage ( Salvia guaranitica L.) overview | 第23页 |
| 1.8 Functional characterization of terpene synthase genes | 第23-24页 |
| 1.9 Soybean Terpene synthase and herbivore-damaged | 第24页 |
| 1.10 Legume plant hormone and the role of strigolactones in nodulation | 第24-25页 |
| 1.11 Aims of the study: The specific aims are as follows | 第25-26页 |
| CHAPTER Ⅱ Materials and methods | 第26-44页 |
| 2.1 Plant materials and tissue collection | 第26页 |
| 2.2 Isolation of chemical compounds | 第26-27页 |
| 2.3 GC-MS analysis of essential oil components | 第27页 |
| 2.4 RNA extraction | 第27-28页 |
| 2.5 cDNA Library preparation and sequencing | 第28-29页 |
| 2.6 Quality control | 第29页 |
| 2.7 De novo trancriptome assembly | 第29页 |
| 2.8 Annotation of unigenes | 第29-30页 |
| 2.9 Differential expression analysis | 第30页 |
| 2.10 Quantitative real-time PCR (qRT-PCR) analysis | 第30-32页 |
| 2.11 Full-length Terpene synthases cDNAs Clones and vectors | 第32-33页 |
| 2.12 Nicotiana plant growth conditions and preparation of Agrobacterium cultures for infection | 第33-35页 |
| 2.13 Arabidopsis plant growth conditions and preparation of Agrobacterium cultures for floral-diptransformation | 第35-36页 |
| 2.14 In vitro soybean hairy root growth conditions and preparation of Agrobacterium cultures forinfection | 第36-37页 |
| 2.15 In viva soybean hairy root growth conditions and preparation of Agrobacterium cultures forinfection | 第37-38页 |
| 2.16 Semiquantitative RT-PCR analysis | 第38-39页 |
| 2.17 Metabolite extraction from transgenic N. tabacum, A. thaliana leaves and in vitro soybean hairyroot | 第39-40页 |
| 2.18 Leaf feeding assay | 第40-41页 |
| 2.19 Determination of tobacco leaf insect feeding using Image-J software | 第41页 |
| 2.20 Statistical analysis | 第41-44页 |
| 2.20.1 First model | 第42页 |
| 2.20.2 Second model | 第42-44页 |
| CHAPTER Ⅲ Results | 第44-113页 |
| 3.1 Identification of essential oil components from S. officinalis and S. guaranitica | 第44-50页 |
| 3.2 Illumina sequencing and de novo assembly of S. officinalis and S. guaranitica leave transcriptome | 第50-51页 |
| 3.3 Functional annotation and classification of S officinalis and S. guaranitica assembled unigenes | 第51-53页 |
| 3.4 KEGG analysis of S. officinalis and S. guaranitica transcriptomes | 第53-55页 |
| 3.5 Genes related to the biosynthesis of isoprenoids from S. officinalis and S. guaranitica | 第55-60页 |
| 3.6 Genes related to terpene synthases from S. officinalis and S. guaranitica | 第60-66页 |
| 3.7 Validation of the gene expression patterns from S. officinalis and S. guaranitica by quantitative RT-PCR | 第66-67页 |
| 3.8 Full-Length Terpene Synthases cDNAs clones and vectors | 第67-70页 |
| 3.9 Functional characterization of terpene synthase genes in transgenic N. tabacum leaves | 第70-73页 |
| 3.10 Functional characterization of terpene synthase genes in transgenic A. thaliana leaves | 第73-84页 |
| 3.11 Functional characterization of terpene synthase genes using transgenic in vitro soybean hairy root | 第84页 |
| 3.12 Leaf feeding assay | 第84-89页 |
| 3.13 Terpene synthase genes conserved motifs prediction | 第89-90页 |
| 3.14 Effects of terpene genes over-expression on soybean root phenotype | 第90-92页 |
| 3.15 Effect of terpene genes over-expression on soybean modulation upon 10-days from B. japonicum(USDA110) infection | 第92页 |
| 3.16 Effect of terpene genes over-expression on soybean nodulation upon 20-days from B.japonicum(USDA110) infection | 第92-98页 |
| 3.17 Effect of terpene genes on SLs biosynthesis and signaling gene expression in the transgenic in vivohairy roots upon 10-days from B. japonicum (USDA110) infection | 第98-105页 |
| 3.18 Effect of terpene genes on SLs biosynthesis and signaling gene expression in the transgenic in vivohairy roots upon 20-days from B. japonicum (USDA110) infection | 第105-110页 |
| 3.19 Effect of terpene genes on SLs biosynthesis and signaling gene expression in nodulation upon 20-days from B. japonicum (USDA110) infection | 第110-113页 |
| CHAPTER Ⅳ Discussion | 第113-127页 |
| Conclusion | 第127-129页 |
| References | 第129-143页 |
| Self-Introduction | 第143页 |
| Publications | 第143-144页 |
| Supplementary Tables and Figures | 第144-156页 |
| Figure S1. Length distributions of transcripts and unigenes in S. officinalis trascriptom | 第144-150页 |
| Figure S2. Length distributions of transcripts and unigenes in S. guaranitica trascriptom | 第150-155页 |
| Figure S3. Blast NR similarity distribution in S. officinalis trascriptome | 第155页 |
| Figure S4. Blast NR similarity distribution in S. guaranitica trascriptome | 第155-156页 |
| Acknowledgements | 第156-157页 |
