| 摘要 | 第6-7页 |
| Abstract | 第7-9页 |
| Chapter 1 Introduction and literature review | 第16-39页 |
| 1.1 Introduction | 第16-27页 |
| 1.1.1 History and origin of wheat | 第16-17页 |
| 1.1.2 Social and economic importance | 第17页 |
| 1.1.3 Wheat cultivation around the world | 第17-19页 |
| 1.1.4 Influence of plant height on wheat production | 第19-20页 |
| 1.1.5 Role of phytohormones on plant growth | 第20-21页 |
| 1.1.6 Factor that favor wheat adaptability | 第21-22页 |
| 1.1.7 Constraints of wheat production | 第22页 |
| 1.1.8 Wheat sharp eyespot | 第22-23页 |
| 1.1.9 Employment of RNA-seq to identify putative sharp eyespot resistant genes | 第23-24页 |
| 1.1.10 Gene introgression from wheat wild relatives | 第24-25页 |
| 1.1.11 Importance of molecular markers in crop breeding | 第25-26页 |
| 1.1.12 Molecular marker development | 第26-27页 |
| 1.1.13 Recombinant inbred line (RIL) populations in breeding research | 第27页 |
| 1.2 Literature review | 第27-38页 |
| 1.2.1 Sharp eyespot | 第27-29页 |
| 1.2.2 Plants and disease | 第29-30页 |
| 1.2.3 Plant defense mechanisms | 第30页 |
| 1.2.4 Signal perception | 第30-31页 |
| 1.2.5 Genes mediated resistances | 第31-32页 |
| 1.2.6 Hypersensitive response (HR) | 第32-33页 |
| 1.2.7 Biochemical defenses | 第33-36页 |
| 1.2.8 Pathogenesis-related proteins | 第36-38页 |
| 1.3 Purpose of the study | 第38-39页 |
| Chapter 2 Silencing of L-type lectin-like receptor kinase gene Ta Lec RK-Ⅳ.1 leads to dwarf phenotype in wheat line CI12633 | 第39-78页 |
| Introduction | 第39-43页 |
| 2.1 Materials and Methods | 第43-56页 |
| 2.1.1 Plant and fungal materials, growth conditions and treatments | 第43页 |
| 2.1.2 RNA Sequencing-based transcriptome analysis | 第43-44页 |
| 2.1.3 Methodology | 第44-53页 |
| 2.1.4 Virus-Induced Gene Silencing | 第53-54页 |
| 2.1.5 BSMV inoculation | 第54-55页 |
| 2.1.6 Test for susceptibility to sharp eyespot | 第55-56页 |
| 2.1.7 Phytohormones treatment (GA, Auxin, ABA and SA) | 第56页 |
| 2.2 Results | 第56-67页 |
| 2.2.1 Identification of Ta Lc RK-Ⅳ.1 by RNA-Seq and cloning of the gene sequence | 第56-58页 |
| 2.2.2 Ta Lc RK encodes an L-type lectin domain containing receptor kinase Ⅳ.1 | 第58-59页 |
| 2.2.3 Chromosomal location of Ta Lec RK -Ⅳ.1 | 第59-60页 |
| 2.2.4 Phenotype resulting from the gene silencing experiment | 第60-62页 |
| 2.2.5 Sharp eyespot responses of the plants subjected to the VIGS experiment | 第62-63页 |
| 2.2.6 Relative expression of Ta Lec RK-Ⅳ.1 and its homologue gene in wheat line with Rht-D1b | 第63-64页 |
| 2.2.7 Analysis of the expression of GA biosynthetic enzymes Ta GA20ox and Ta GA3ox2 in Rht2 wheat line and CI12633 plants inoculated with BSMV:Ta Lec RK-Ⅳ.1 | 第64-65页 |
| 2.2.8 Expression patterns of Ta Lec RK-Ⅳ.1 in response to exogenous hormone treatments | 第65-67页 |
| Discussion | 第67-78页 |
| Chapter 3 Analysis for quantitative loci using molecular markers derived from wheat genes induced by sharp eyespot | 第78-95页 |
| Introduction | 第78-79页 |
| 3.1 Materials and methods | 第79-85页 |
| 3.1.1 Plant materials | 第79页 |
| 3.1.2 Pathogen material | 第79页 |
| 3.1.3 Pathogen tests | 第79-80页 |
| 3.1.4 Pathogen assessment | 第80-81页 |
| 3.1.5 DNA isolation and PCR amplification | 第81-84页 |
| 3.1.6 Linkage map construction and QTL analysis | 第84-85页 |
| 3.2 Results | 第85-91页 |
| 3.2.1 Phenotypic assessments of sharp eyespot infection | 第85-87页 |
| 3.2.2 Marker development, genotyping and linkage map construction | 第87-89页 |
| 3.2.3 Analysis of the phenotypic and genotypic data for QTL associated with the resistance trait | 第89-91页 |
| Discussion | 第91-95页 |
| Chapter 4 General discussion | 第95-101页 |
| Summary | 第101-102页 |
| References | 第102-117页 |
| Acknowledgements | 第117-118页 |
| Funding | 第118页 |
| Manuscript in preparation | 第118页 |
