| 摘要 | 第1-7页 |
| ABSTRACT | 第7-11页 |
| Chapter Ⅰ Literature Review | 第11-22页 |
| ·Purpose and significance of this research | 第11页 |
| ·Introduction to the development of molecular markers | 第11-13页 |
| ·RFLP and DNA fingerprinting | 第11-12页 |
| ·Class Ⅱ molecular marker based on PCR | 第12页 |
| ·Class Ⅲ molecular marker based on sequencing and other markers | 第12-13页 |
| ·Principles and characteristics of molecular genetic markers used in the current research | 第13页 |
| ·The transforming growth factor β(TGF-β) superfamily | 第13-22页 |
| ·BMP Family | 第14-15页 |
| ·Growth differentiation factor (GDF) subfamily | 第15-16页 |
| ·GDF10 gene | 第16-22页 |
| Chapter Ⅱ Material and methods | 第22-31页 |
| ·Materials | 第22页 |
| ·Experimental animals | 第22页 |
| ·Body measurements traits | 第22页 |
| ·Main equipment and reagent | 第22-23页 |
| ·Main equipment | 第22-23页 |
| ·Major reagent and kits | 第23页 |
| ·Experimental methods | 第23-27页 |
| ·DNA extraction and detection | 第23-24页 |
| ·PCR amplification | 第24-26页 |
| ·SSCP analysis | 第26-27页 |
| ·Statistical analysis | 第27-31页 |
| ·Calculation of allele and genotype frequencies & Hardy-Weinberg equilibrium theory | 第27-30页 |
| ·Effective number of alleles (Ne) | 第30页 |
| ·Polymorphic informative content | 第30页 |
| ·Homozygosity and heterozygosity | 第30页 |
| ·Analysis of body measurement traits | 第30-31页 |
| Chapter Ⅲ Results and analysis | 第31-43页 |
| ·Extraction of blood's genomic DNA | 第31页 |
| ·Detection of polymorphism in GDF10 gene exon1, 2 and 3 | 第31-36页 |
| ·Detection of polymorphism in GDF10 gene exon1 | 第31页 |
| ·Detection of polymorphism in GDF10 gene exon2 | 第31-33页 |
| ·Detection of polymorphism in GDF10 gene exon3 | 第33-34页 |
| ·Polymorphism's sequence analysis | 第34-36页 |
| ·Mendelian factor determination and analysis of association between SNPs and Body measurement trait | 第36-43页 |
| ·Genetic analysis of GDF10 gene Exon1 (142bp locus) and effect of SNP on body measurement traits | 第37-38页 |
| ·Genetic analysis of GDF10 gene Exon3 and effect of SNP on body measurement traits | 第38-43页 |
| Discussion | 第43-45页 |
| Conclusion | 第45-46页 |
| References | 第46-50页 |
| Acknowledgements | 第50-51页 |
| Self introduction | 第51页 |
