| 摘要 | 第6-8页 |
| ABSTRACT | 第8-9页 |
| Chapter one: Introduction | 第14-21页 |
| 1.1 Background | 第14页 |
| 1.2 Literature review | 第14-19页 |
| 1.2.1 Abiotic stress | 第14-17页 |
| 1.2.2 WRKY transcription factor | 第17-18页 |
| 1.2.3 Research progress in grapes | 第18-19页 |
| 1.3 Problem statement | 第19页 |
| 1.4 Justification of study | 第19页 |
| 1.5 General objective | 第19-21页 |
| Chapter two: Expression, transformation and phenotypic analysis | 第21-36页 |
| 2.1 Introduction | 第21-22页 |
| 2.2 Materials and methods | 第22-30页 |
| 2.2.1 Plant materials | 第22页 |
| 2.2.2 Cold and drought stresses treatment | 第22-23页 |
| 2.2.3 RNA extraction and quantitative real-time PCR (qRT-PCR) analysis | 第23-25页 |
| 2.2.4 Transformation of Arabidopsis | 第25-29页 |
| 2.2.4.1 Arabidopsis plant materials and growth conditions | 第25页 |
| 2.2.4.2 Vector construction | 第25-27页 |
| 2.2.4.3 Arabidopsis transformation | 第27页 |
| 2.2.4.4 Gene expression assay in transgenic Arabidopsis | 第27-29页 |
| 2.2.5 Phenotypic evaluation of transgenic Arabidopsis under abiotic stress | 第29-30页 |
| 2.3 Results | 第30-33页 |
| 2.3.1 Analysis the ORFs and deduced amino acid sequences of VaWRKY55 | 第30页 |
| 2.3.2 Cold and drought stress induces the expression of VaWRKY55 in grapevine | 第30-31页 |
| 2.3.3 Screening the positive transgenic lines | 第31-32页 |
| 2.3.4 Overexpression of VaWRKY55 enhances drought tolerance in transgenic Arabidopsis | 第32-33页 |
| 2.4 Discussion | 第33-36页 |
| Chapter three: Physiological and structural changes in VaWRKY55 overexpressing Arabidopsis under drought stress | 第36-46页 |
| 3.1 Introduction | 第36页 |
| 3.2 Materials and methods | 第36-40页 |
| 3.2.1 Determination of proline content | 第37-38页 |
| 3.2.2 Determination of the soluble sugar content | 第38页 |
| 3.2.3 Determination of hydrogen peroxide content | 第38-39页 |
| 3.2.4 Evaluation of the electrolyte leakage | 第39页 |
| 3.2.5 Measurement of relative water content (RWC) | 第39页 |
| 3.2.6 Measurement of the leaf water loss | 第39-40页 |
| 3.2.7 Estimation of the stomata density | 第40页 |
| 3.3 Results | 第40-43页 |
| 3.3.1 Elevated soluble sugars content | 第40页 |
| 3.3.2 Increased levels of proline in transgenic Arabidopsis plants | 第40页 |
| 3.3.3 Reduced hydrogen peroxide contents | 第40-41页 |
| 3.3.4 Reduced electrolyte leakage in transgenic Arabidopsis | 第41-42页 |
| 3.3.5 Reduced leaf stomata density in transgenic Arabidopsis plants | 第42页 |
| 3.3.6 Abundant relative water content in transgenic Arabidopsis plants | 第42页 |
| 3.3.7 Reduced rate of leaf water loss in transgenic Arabidopsis | 第42-43页 |
| 3.4 Discussion | 第43-46页 |
| Chapter four: Transcriptome analysis of transgenic Arabidopsis | 第46-54页 |
| 4.1 Introduction | 第46页 |
| 4.2 Materials and methods | 第46-49页 |
| 4.2.1 Plant materials and growth conditions | 第46-47页 |
| 4.2.2 RNA isolation | 第47页 |
| 4.2.3 The cDNA library constructions and sequencing | 第47-48页 |
| 4.2.4 Data analysis | 第48-49页 |
| 4.3 Results | 第49-52页 |
| 4.3.1 Classification of the differentially expressed genes | 第49-51页 |
| 4.3.2 Confirmation of RNA-seq data | 第51-52页 |
| 4.4 Discussion | 第52-54页 |
| Chapter Five:Conclusion and prospects | 第54-56页 |
| 5.1. Conclusion | 第54-55页 |
| 5.2. Prospects | 第55-56页 |
| References | 第56-62页 |
| Appendix | 第62-63页 |
| Personal information and publications | 第63-64页 |
| Acknowledgement | 第64页 |
