| 致谢 | 第1-5页 |
| 摘要 | 第5-7页 |
| Abstract | 第7-10页 |
| Contents | 第10-13页 |
| Chapter 1 Background | 第13-40页 |
| ·Phytochemicals and bioactivities of Anemone raddeana Rhizome | 第13-31页 |
| ·Phytochemistry | 第14-19页 |
| ·Bioactivity and pharmacological properties | 第19-29页 |
| ·Improvement in methods of extracting and detecting triterpenoid saponins | 第29-31页 |
| ·Quality control of traditional Chinese medicine | 第31-38页 |
| ·Thin layer chromatography | 第33页 |
| ·High-performance liquid chromatography | 第33-34页 |
| ·Gas chromatography | 第34页 |
| ·Electrophoretic methods | 第34-35页 |
| ·Hyphenated techniques | 第35-36页 |
| ·Spectroscopic techniques | 第36-37页 |
| ·Fingerprint | 第37-38页 |
| ·The aim and objective of the thesis | 第38-40页 |
| Chapter 2 Determination of Raddeanin A by TLC and HPLC | 第40-47页 |
| ·Introduction | 第40页 |
| ·Materials, chemicals and reagents | 第40-41页 |
| ·Preparation of standard solutions | 第41页 |
| ·Preparation of sample solutions | 第41页 |
| ·TLC analysis of A. raddeana Rhizome samples | 第41页 |
| ·HPLC analysis of A. raddeana Rhizome samples | 第41-44页 |
| ·Apparatus and Chromatographic Conditions | 第42页 |
| ·Calibration curve | 第42-43页 |
| ·The limit of detection (LOD) and the limit of quantification (LOQ) | 第43页 |
| ·Precision test | 第43页 |
| ·Stability test | 第43-44页 |
| ·Reproducibility test | 第44页 |
| ·Recovery test | 第44页 |
| ·Results and discussion | 第44-47页 |
| Chapter 3 HPLC and quality control of A. raddeana Rhizome | 第47-53页 |
| ·Introduction | 第47-48页 |
| ·Theory | 第48-49页 |
| ·Establishment of the HPLC fingerprint of A. raddeana Rhizome | 第49-51页 |
| ·Materials and methods | 第50页 |
| ·Determination of HPLC fingerprint | 第50-51页 |
| ·Results and discussion | 第51-53页 |
| Chapter 4 FT-IR spectroscopy and quality control of A. raddeana Rhizome | 第53-57页 |
| ·Introduction | 第53页 |
| ·Theory | 第53-54页 |
| ·Establishment of FT-IR fingerprint of A. raddeana Rhizome | 第54-56页 |
| ·Materials and methods | 第54页 |
| ·Determination of FT-IR fingerprint | 第54-56页 |
| ·Results and discussion | 第56-57页 |
| Chapter 5 UV spectroscopy and quality control of A. raddeana Rhizome | 第57-60页 |
| ·Introduction | 第57页 |
| ·Theory | 第57-58页 |
| ·Establishment of UV fingerprint of A. raddeana Rhizome | 第58页 |
| ·Materials and methods | 第58页 |
| ·Determination of UV fingerprint | 第58页 |
| ·Results and discussion | 第58-60页 |
| Chapter 6 Comprehensive analysis of HPLC,FT-IR and UV fingerprints | 第60-63页 |
| ·Introduction | 第60-61页 |
| ·Cluster analysis of HPLC, FT-IR and UV fingerprints | 第61页 |
| ·Results and discussion | 第61-63页 |
| Chapter 7 Determination of the toxicity of A raddeana Rhizome | 第63-71页 |
| ·Introduction | 第63-64页 |
| ·Experiment | 第64-70页 |
| ·Extraction of A. raddeana Rhizome | 第64页 |
| ·Laboratory mice | 第64页 |
| ·Toxicity study | 第64-65页 |
| ·Acute toxic symptoms | 第65页 |
| ·Determination of the LD_(50) of the water extract of A. raddeana Rhizome | 第65-66页 |
| ·Toxicity classification of A. raddeana Rhizome | 第66-67页 |
| ·Determination of the toxicity of the organic solvent extract of A. raddeana Rhizome | 第67-70页 |
| ·Results and discussion | 第70-71页 |
| Chapter 8 Conclusions and outlook | 第71-75页 |
| ·Fingerprinting of A. raddeana Rhizome | 第71-72页 |
| ·Toxicity of A. raddeana Rhizome | 第72-73页 |
| ·The innovation in this thesis | 第73页 |
| ·Drawbacks and improvement | 第73-75页 |
| Reference | 第75-86页 |
| Appendix | 第86-87页 |
| 作者简历 | 第87页 |
