| ABSTRACT | 第1-6页 |
| 摘要 | 第6-8页 |
| CONTENTS | 第8-11页 |
| Chapter 1 Introduction | 第11-36页 |
| ·Acid mine waters | 第11-12页 |
| ·Origins | 第11页 |
| ·Chemical charateristics | 第11-12页 |
| ·Microbial diversity of acid mine waters | 第12-16页 |
| ·Bacteria | 第13-16页 |
| ·Archaea | 第16页 |
| ·Methods of study of microbes in acid mine waters | 第16-24页 |
| ·Analysis of rRNA molecules | 第16-19页 |
| ·Cultivation approach | 第19-22页 |
| ·In situ hybridization(FISH) | 第22-24页 |
| ·Interaction between microbes and minerals | 第24-33页 |
| ·Synergistic interactions involving iron, sulfur, and carbon oxidizers | 第24-26页 |
| ·Contact leaching | 第26-30页 |
| ·Cooperative leaching strategy | 第30-31页 |
| ·A. ferrooxidans adsorbed on pyrite | 第31-33页 |
| ·Significance and content of the project | 第33-36页 |
| ·Research background and significance | 第33-35页 |
| ·Research contents | 第35-36页 |
| Chapter 2 Materials and Methods | 第36-47页 |
| ·Acid mine water samples | 第36页 |
| ·Site description and sample collection | 第36页 |
| ·Chemical elemental analysis of the samples | 第36页 |
| ·Culture medium | 第36-38页 |
| ·Liquid medium | 第37页 |
| ·Solid medium | 第37-38页 |
| ·Pyrite sample | 第38页 |
| ·Experimental | 第38-47页 |
| ·16S rDNA libraries'construction | 第38-40页 |
| ·Isolation and identification for bacterial strains | 第40-41页 |
| ·Adsorption of microbes on pyrite | 第41-47页 |
| Chapter 3 Microbial communities in acid mine water from Zhongtiaoshan copper mine | 第47-64页 |
| ·Biogeochemical properties of samples | 第47-48页 |
| ·Obtaining 16S rDNA sequences from samples | 第48页 |
| ·RFLP analysis of 16S rDNA cloning libraries | 第48-51页 |
| ·Sequencing analysis of 16S rDNA clone libraries | 第51-54页 |
| ·Phylogenetic analysis | 第54-60页 |
| ·Discussion | 第60-63页 |
| ·Summary | 第63-64页 |
| Chapter 4 Microbial communities in acid mine water from Tongshankou copper mine | 第64-83页 |
| ·Biogeochemical properties of samples | 第64页 |
| ·DNA extraction and 16S rDNA PCR | 第64-65页 |
| ·RFLP and sequencing analysis | 第65-74页 |
| ·Phylogenetic relationship of bacterial sequence types | 第74-77页 |
| ·Phylogenetic analysis of archaeal sequences | 第77-79页 |
| ·Discussion | 第79-81页 |
| ·Summary | 第81-83页 |
| Chapter 5 Isolation and identification of Leptospirillum ferriphilum and Acidiphilium sp | 第83-90页 |
| ·Isolation and identification of Leptospirillum ferriphilum | 第83-86页 |
| ·Isolation by plating | 第83页 |
| ·Morphology observation | 第83-85页 |
| ·16S rDNA analysis | 第85-86页 |
| ·Isolation and identification of Acidiphilium sp. | 第86-88页 |
| ·Isolation by plating | 第86-87页 |
| ·Morphology observation | 第87-88页 |
| ·Phylogenetic analysis | 第88页 |
| ·Summary | 第88-90页 |
| Chapter 6 Adsorption of microbes on pyrite surface | 第90-105页 |
| ·Observation of pyrite surface before adsorption | 第90-91页 |
| ·Appearance observation | 第90页 |
| ·Energy spectrum analysis | 第90-91页 |
| ·Adsorption | 第91-92页 |
| ·Observation of pyrite surface after adsorption | 第92-95页 |
| ·Appearance observation | 第92-93页 |
| ·Energy spectrum analysis | 第93-95页 |
| ·Observation of microbes adsorbed on pyrite surface | 第95-100页 |
| ·Microbes adsorbed on pyrite surface from different adsorption systems after 16 days' adsorption | 第95-96页 |
| ·Microbes adsorbed on pyrite surface from adsorption system with K2 sample after adsorption for different time | 第96-100页 |
| ·Detection of microbes' adsorption on pyrite surface by FISH | 第100-104页 |
| ·Positive control | 第100-101页 |
| ·Detection of microbes' adsorption on pyrite surface | 第101-104页 |
| ·Summary | 第104-105页 |
| Chapter 7 Conclusions | 第105-107页 |
| REFERENCES | 第107-115页 |
| ACKNOWLEGEMENTS | 第115-116页 |
| MAIN RESEARCH PRODUCTION | 第116-118页 |
| 附：3000字中文摘要 | 第118-120页 |
