| ABSTRACT | 第7-8页 |
| 摘要 | 第9-13页 |
| Review and Literature | 第13-22页 |
| Chapter Ⅰ:The ER stress and Effects of Lipopolysaccharide and Zearalenone | 第13-22页 |
| 1.Endoplasmic reticulum stress | 第14页 |
| 2.Pre-implantation embryos development | 第14-15页 |
| 3.Lipopolysaccharide and/or Zearalenone induced cell apoptosis | 第15-16页 |
| 4.Lipopolysaccharide and/or Zearalenone induced cell proliferation | 第16-17页 |
| 5.Lipopolysaccharide and/or Zearalenone induced autophagy | 第17页 |
| 6.Lipopolysaccharide and/or Zearalenone induced activation of ER stress | 第17-18页 |
| 7.Lipopolysaccharide and/or Zearalenone induced inflammatory cytokines | 第18-20页 |
| 8.Some ways to blocked endoplasmic reticulum stress | 第20-22页 |
| Experimental research | 第22-48页 |
| Chapter Ⅱ:Endoplasmic Reticulum Stress is Involved in Lipopolysaccharide Induced Inflammatory Response and Apoptosis in Goat Endometrial Stromal Cells | 第22-40页 |
| 1.MATERIALS AND METHODS | 第23-27页 |
| 1.1 Cell culture and drug treatment | 第23页 |
| 1.2 Cell viability | 第23-24页 |
| 1.3 Apoptosis analysis | 第24页 |
| 1.4 Total RNA extraction and real-time quantitative PCR | 第24-25页 |
| 1.5 ELISA | 第25页 |
| 1.6 Western blot analysis | 第25-26页 |
| 1.7 Knock down ATF6 and IRE1 genes with small interfering RNA | 第26-27页 |
| 1.8 Statistical analysis | 第27页 |
| 2.RESULTS | 第27-36页 |
| 2.1 Functional role of LPS on ESC cell proliferation | 第27-28页 |
| 2.2 Effect of LPS on ESC apoptosis and PCNA,MKI67 expression | 第28-29页 |
| 2.3 LPS activated Endoplasmic reticulum stress | 第29-30页 |
| 2.4 LPS affected on expression of autophagy and mitochondria-related genes | 第30-31页 |
| 2.5 LPS increased inflammatory cytokines secretion | 第31-34页 |
| 2.6 4-phenylbutyrate inhibited the expression of ER stress protein,autophagy and pro-inflammatory cytokines under LPS treatment | 第34-35页 |
| 2.7 Effect of ATF6 and IRE1 on promoting autophagy | 第35-36页 |
| DISCUSSION | 第36-40页 |
| Chapter Ⅲ:Endoplasmic Reticulum Stress is Involved in Mycotoxin Zearalenone Induced Inflammatory Response,Proliferation,and Apoptosis in Goat Endometrial Stromal Cells | 第40-48页 |
| 1.Materials and methods | 第41-43页 |
| 1.1 Chemicals | 第41页 |
| 1.2 Cell culture and treatments | 第41页 |
| 1.3 Cell proliferation | 第41页 |
| 1.4 Cell apoptosis assay | 第41-42页 |
| 1.5 ELISA | 第42页 |
| 1.6 Western blot | 第42页 |
| 1.7 Knockdown ERN1 gene by small interfering RNA | 第42-43页 |
| 1.8 Statistical analysis | 第43页 |
| 2.Results | 第43-47页 |
| 2.1 Dynamic role of ZEA on ESC proliferation and apoptosis assay | 第43-44页 |
| 2.2 Endoplasmic reticulum stress-activated by ZEA on Goat endometrial stromal cells | 第44-45页 |
| 2.3 Autophagy and inflammatory cytokine affected with ZEA on ESCs | 第45-46页 |
| 2.4 knockdown IRE1 and affected on promoting autophagy and inflammation | 第46-47页 |
| DISCUSSION | 第47-48页 |
| Conclusion | 第48-49页 |
| General Conclusion | 第49-50页 |
| REFERENCES | 第50-62页 |
| Acknowledgements | 第62-63页 |
| 作者简介 | 第63-64页 |
