| ABSTRACT | 第10-14页 |
| 摘要 | 第15-18页 |
| Nomenclature and Abbreviations | 第18-20页 |
| PREFACE | 第20-22页 |
| CHAPTER 1 AUTOIMMUNE POLY-GLANDULAR SYNDROME (APS) | 第22-34页 |
| Epidemiology | 第22-23页 |
| Clinical aspects of APS | 第23页 |
| Hereditary susceptibility of APS | 第23-24页 |
| Biological mechanisms of APS | 第24-25页 |
| Thyroid disorders and diabetes mellitus | 第25页 |
| Regularity of thyroid dysfunctions in the general population and in patients with diabetes | 第25-26页 |
| Glucose homeostasis under the influence of thyroid hormones | 第26-28页 |
| Alterations of thyroid hormones and related diseases due to concomitant presence of diabetesmellitus | 第28-29页 |
| Inter relationship between DM and TD and importance of Claudin-11 in BTB,throughreview of figures | 第29-34页 |
| CHAPTER 2 Effects of contemporaneous diabetes mellitus cum hyperthyroidism on the fertilityof male mice and immuno-localization of Claudin-11 inside the seminiferous tubules | 第34-58页 |
| Introduction | 第34-35页 |
| Materials and Methods | 第35-39页 |
| Ethics statement | 第35页 |
| Animals and treatments | 第35-36页 |
| Sample collection | 第36-37页 |
| Measurement of serum hormones | 第37页 |
| Histo-morphology under light microscope | 第37页 |
| Immunolocalization of Oligodendrocyte-specific protein/Claudin-11 in mouse testis | 第37-38页 |
| IHC quantification through digital image analysis | 第38-39页 |
| Statistical analysis | 第39页 |
| Results | 第39-50页 |
| Body, testes, and epididymides weights and testis/body weight ratio | 第39-41页 |
| Blood glucose levels | 第41-42页 |
| Food and water consumption | 第42页 |
| Serum hormonal profile | 第42-44页 |
| Histological evaluation of testicular sections | 第44-47页 |
| Measurement of immuno- localization of Claudin-11 | 第47-48页 |
| Histological evaluation of epididymal sections | 第48-50页 |
| Discussion | 第50-56页 |
| Conclusion | 第56-58页 |
| CHAPTER 3 Effects of concomitant diabetes mellitus plus hypothyroidism on the fertility of malemice and immuno-localization of Claudin-11 in the testes | 第58-78页 |
| Introduction | 第58-59页 |
| Materials and Methods | 第59-61页 |
| Ethics statement | 第59页 |
| Animals and treatments | 第59-60页 |
| Sample collection | 第60页 |
| Measurement of serum hormones | 第60页 |
| Histo-morphology under light microscope | 第60-61页 |
| Immuno-localization of Oligodendrocyte-Specific Protein/Claudin-11 in the testes of mouse | 第61页 |
| IHC quantification through digital image analysis | 第61页 |
| Statistical analysis | 第61页 |
| Results | 第61-72页 |
| General conditions | 第61-62页 |
| Relative body and testes weights | 第62页 |
| Blood glucose levels | 第62-63页 |
| Food and water consumption | 第63-64页 |
| Hormonal findings by RIA | 第64-65页 |
| Histo-morphological evaluation of testicular and epididymal sections | 第65-70页 |
| Measurement of immuno- localization of Claudin-11 | 第70-72页 |
| Discussion | 第72-77页 |
| Conclusion | 第77-78页 |
| CHAPTER 4 Effects of concurrent hyperthyroidism and diabetes mellitus on the fertility of malemice from pre- to post-pubertal periods | 第78-106页 |
| Introduction | 第78-79页 |
| Materials and Methods | 第79-82页 |
| Ethics statement | 第79页 |
| Experimental animals and treatments | 第79页 |
| Collection of samples | 第79-80页 |
| Biochemical assays | 第80页 |
| Sperm quality assessment | 第80页 |
| Sample preparation | 第80-81页 |
| Sperm count | 第81页 |
| Sperm motility | 第81页 |
| Histo-morphometric analyses | 第81-82页 |
| Statistical analysis | 第82页 |
| Results | 第82-96页 |
| Body, testes and epididymal weights | 第82-84页 |
| Blood glucose levels | 第84页 |
| Hormonal evaluation | 第84-86页 |
| Morpho-metric measurements of testicular and epididymal tissues at different age levels | 第86-89页 |
| Comparative Histo-pathology of mice testes | 第89-91页 |
| Histo-morphology of epididymis of pre- and post-pubertal mice | 第91-94页 |
| Sperm count and motility measurements | 第94-96页 |
| Discussions | 第96-103页 |
| Conclusion | 第103-106页 |
| CHAPTER 5 Effects of coexisting Diabetes Mellitus and Hypothyroidism on testicular andepididymal histoarchitecture and spermatogenesis of neonatal to adult mice | 第106-132页 |
| Introduction | 第106-107页 |
| Materials and Methods | 第107-110页 |
| Ethics statement | 第107页 |
| Experimental animals and treatments | 第107-108页 |
| Collection of samples | 第108页 |
| Biochemical assays | 第108-109页 |
| Sperm quality assessment | 第109页 |
| Sample preparation | 第109页 |
| Sperm count | 第109页 |
| Sperm motility | 第109页 |
| Histo-morphometric analyses | 第109-110页 |
| Statistical analysis | 第110页 |
| Results | 第110-125页 |
| Body, testes and epididymal weights | 第110-112页 |
| Blood glucose concentrations | 第112-113页 |
| Hormonal evaluation | 第113-114页 |
| Morpho-metric measurements of testicular and epididymal tissues at different age levels | 第114-117页 |
| Comparative histo-pathology of mice testes | 第117-118页 |
| Histo-morphology of CpE and CdE in the mice of 24d vs 56d old | 第118-123页 |
| Sperm count and motility observations | 第123-125页 |
| Discussions | 第125-130页 |
| Conclusion | 第130-132页 |
| Conclusion | 第132-134页 |
| Innovation | 第134-136页 |
| Suggested future studies | 第136-138页 |
| List of published, submitted and under revision papers | 第138-140页 |
| References | 第140-152页 |
| Acknowledgement | 第152-154页 |
| Appendix Laboratorial Procedures | 第154-159页 |
