| List of Abbreviations | 第9-11页 |
| 摘要 | 第11-14页 |
| Abstract | 第14-15页 |
| Chapter 1 Introduction and Review of literature | 第17-30页 |
| 1.1 Geminiviruses: General description | 第17页 |
| 1.2 Geminiviruses:Txonmy | 第17-18页 |
| 1.3 Geminiviruses: Genome organization | 第18-21页 |
| 1.4 Geminiviruses: gene function and host-virus interaction | 第21-25页 |
| 1.5 Geminiviruses: Ecology | 第25-26页 |
| 1.6 Geminiviruses in China and Fujian Province | 第26-29页 |
| 1.7 Objectives of Research | 第29-30页 |
| Chapter 2 Detection of Begomoviruses from different host plants in Fujian Province ofChina | 第30-54页 |
| 2.1 Background | 第30-31页 |
| 2.2 Materials and methods | 第31-36页 |
| 2.2.1 Collection of samples | 第31-32页 |
| 2.2.2 Genomic DNA extraction | 第32页 |
| 2.2.3 PCR amplification, cloning, and sequencing | 第32-34页 |
| 2.2.4 PCR/RCA amplification of DNA P | 第34-35页 |
| 2.2.5 Phylogenetic analysis | 第35-36页 |
| 2.3 Results and Discussion | 第36-54页 |
| 2.3.1 Identification of Begomoviruses and Satellites in Fujian Province of China | 第36-54页 |
| 2.3.1.1 Detection of Cotton leaf curl Multan virus and Betasatellite from Hibiscussabdariffa | 第38-42页 |
| 2.3.1.2 Detection of Ramie mosaic virus (RamV) from Ramie (Boehmeria nivea) | 第42-44页 |
| 2.3.1.3 Detection of Tomato yellow leaf curl virus (TYLCV) from Tomato plants(Lycopersicon esculentum L) | 第44-46页 |
| 2.3.1.4 Detection of Corchorus yellow vein Vietnam virus (CoYVV) from Jute plants(Corchorus olitorius L.) | 第46-48页 |
| 2.3.1.5 Detection of Ageratum yellow vein China virus (AYVCnV) infecting Ageratum(Ageratum conyzoides) | 第48-52页 |
| 2.3.1.6 Detection of Kudzu mosaic virus virus (KuMV) infecting Kudzu (Ageratumconyzoides) | 第52-54页 |
| Chapter 3 Construction of Infectious clone and Molecular studies/characterization ofCLCuMuV, RamV and CoYVV | 第54-63页 |
| 3.1 Background | 第54-55页 |
| 3.2 Material and Methods | 第55-57页 |
| 3.2.1 Collection of samples and DNA extraction | 第55-56页 |
| 3.2.2 Construction of infectious clone and agrobacterium infilteration | 第56-57页 |
| 3.2.3 Confirmation of infection by Southern Blot analysis | 第57页 |
| 3.2.4 Cloning and sequence analysis: | 第57页 |
| 3.3 Results and Discussion | 第57-63页 |
| Chapter 4 Molecular characterization and RSV Co-infect Nicotiana benthamiana withbegomoviruses | 第63-74页 |
| 4.1 Background | 第63-65页 |
| 4.2 Material and Methods | 第65-66页 |
| 4.2.1 Virus Source and inoculation | 第65页 |
| 4.2.2 Detection of TSSs by Southern Blot analysis | 第65页 |
| 4.2.3 Primer Design | 第65-66页 |
| 4.2.4 High Throughput sequencing of 5' ends of CLCuMuV, RamV and CoYVVNCP | 第66页 |
| 4.3 Results and Discussion | 第66-74页 |
| 4.3.1 Rice stripe virus Co-infect Nicotiana benthamina with Cotton leaf curl Multan virus(CLCuMuV), Ramie mosaic virus (RamV), Corchorus yellow vein Vietnam virus(CoYVV) | 第67-68页 |
| 4.3.2 RSV snatches capped RNA leaders from CLCuMuV, RamV and CoYVVmRNAs | 第68-74页 |
| Chapter 5 General Discussion | 第74-82页 |
| Chapter 6 Conclusions and Future perspective of this study | 第82-85页 |
| 6.1 Conclusion | 第82-84页 |
| 6.2 Future Perspective | 第84-85页 |
| References | 第85-96页 |
| Published Papers and other Academic Achievements during the Study | 第96-98页 |
| Acknowledgement | 第98-99页 |
| Appendices | 第99-115页 |
| Appendix A | 第99-110页 |
| Appendix B | 第110-115页 |
