| 摘要 | 第4-6页 |
| 英文摘要 | 第6-8页 |
| Chapter 1 Introduction | 第12-30页 |
| 1.1 Cell membrane components and structure | 第12-16页 |
| 1.1.1 Lipids | 第12-15页 |
| 1.1.2 Membrane protein | 第15-16页 |
| 1.2 Cell membrane properties and function | 第16-20页 |
| 1.2.1 Membrane's mechanical and thermodynamics properties | 第16-18页 |
| 1.2.2 Transport and signaling function of the membrane | 第18-20页 |
| 1.3 Cholesterol in membrane | 第20-22页 |
| 1.3.1 The structure and metabolism of cholesterol in mammal cell | 第20-21页 |
| 1.3.2 The distribution and role of cholesterol in membrane | 第21-22页 |
| 1.4 Intracellular transport and membrane remodeling protein | 第22-25页 |
| 1.4.1 Main ways of the intracellular vesicular transport | 第22-23页 |
| 1.4.2 Mechanisms of membrane remodeling | 第23-25页 |
| 1.5 Interactions of membrane with the foreigner molecules-peptide or par-ticles | 第25-30页 |
| 1.5.1 Antimicrobial peptide and cell penetrating peptide | 第25-27页 |
| 1.5.2 Formation of membrane pore formation | 第27-28页 |
| 1.5.3 Biological nanotechnology and drug delivery | 第28-30页 |
| Chapter 2 Methods | 第30-42页 |
| 2.1 Fundamental principle of computer simulation | 第30-34页 |
| 2.1.1 Calculating averages from a molecular dynamics simulation | 第30-32页 |
| 2.1.2 Classical mechanics | 第32-34页 |
| 2.2 Force field | 第34-39页 |
| 2.2.1 Genetal principle of force field | 第34-35页 |
| 2.2.2 All atom simulation | 第35-37页 |
| 2.2.3 Coarse grained simulation | 第37-39页 |
| 2.3 Calculation of free energy in MD simulation | 第39-42页 |
| Chapter 3 Influence of different membrane environments on the behavior of cholesterol | 第42-62页 |
| 3.1 Introduction | 第42-43页 |
| 3.2 Methods and Models | 第43-47页 |
| 3.3 Results and Discussion | 第47-57页 |
| 3.3.1 Cholesterol condensing effect | 第47-48页 |
| 3.3.2 Thermodynamics analysis of cholesterol in different membranes | 第48-53页 |
| 3.3.3 Redistribution of cholesterol in curved membrane and discus-sion on its implication | 第53-57页 |
| 3.4 Conclusion | 第57-58页 |
| 3.5 Appendix | 第58-62页 |
| Chapter 4 Translocation of poly-arginine and conjugated particles across the asymmetric membrane | 第62-75页 |
| 4.1 Introduction | 第62-63页 |
| 4.2 Methods and Models | 第63-67页 |
| 4.3 Results and Discussion | 第67-73页 |
| 4.3.1 Translocation of peptides across asymmetric membranes | 第67-69页 |
| 4.3.2 Translocation of peptide and its conjugated particle across asym-metric membranes | 第69-73页 |
| 4.4 Conclusion | 第73-75页 |
| Chapter 5 Computer simulations of the N-terminal helices of Epsin, Sarlp and Arfl interacting with the membrane | 第75-90页 |
| 5.1 Introduction | 第75-79页 |
| 5.2 Methods | 第79-81页 |
| 5.3 Results and Disussion | 第81-89页 |
| 5.3.1 Insertion of amphiphilic helix into membrane | 第81-85页 |
| 5.3.2 Effect of helix insertion on lipid packing of membrane | 第85-86页 |
| 5.3.3 Membrane remodeling upon insertion of multiple helices | 第86-89页 |
| 5.4 Conclusion | 第89-90页 |
| Chapter 6 Conclusion and Outlook | 第90-94页 |
| Bibliography | 第94-114页 |
| Publications | 第114-116页 |
| Acknowledgement | 第116-118页 |
