| 摘要 | 第1-5页 |
| Abstract | 第5-6页 |
| 符号说明 | 第6-7页 |
| 目录 | 第7-10页 |
| CHAPTER 1 Review of Related Literature | 第10-30页 |
| ·Background | 第11-12页 |
| ·Biodiesel from high oil content microalgae---the best one for these issues | 第12页 |
| ·Feedstock of biodiesel | 第12-15页 |
| ·First generation biodisel feedstock | 第13页 |
| ·Second generation biodiesel feedstock | 第13-14页 |
| ·Third generation biodiesel feedstock | 第14-15页 |
| ·Microalgae for Carbon dioxide capture | 第15-17页 |
| ·Global warming | 第15页 |
| ·Physical methods | 第15-16页 |
| ·Chemical methods | 第16页 |
| ·Biological methods | 第16-17页 |
| ·Advances of microalga-bassed systems for carbon dioxide fixation and biofuels-producing | 第17-28页 |
| ·Upstream manipulations | 第18-22页 |
| ·Screening and breeding of microalgae | 第18-20页 |
| ·Genetic engineering of microalgae | 第20-22页 |
| ·Middlestream minipulations | 第22-26页 |
| ·Forms of metabolism | 第23页 |
| ·Reactor design | 第23-25页 |
| ·Nutrient profile manipulation | 第25-26页 |
| ·Downstream manipulation | 第26-28页 |
| ·Cell harvesting metgodologies | 第26-27页 |
| ·Cell disruption and oil extraction | 第27-28页 |
| ·Oiltransesterification | 第28页 |
| ·Bottlenecks that preclude the full industrial exploitation of microalgal cells | 第28-29页 |
| ·The purpose and contents of the article | 第29-30页 |
| CHAPTER 2 How does simulated flue gases affect on the growth of Chlorella? | 第30-48页 |
| ·Introduction | 第31页 |
| ·Materials and methods | 第31-39页 |
| ·Reagents and Equipments | 第31-33页 |
| ·Strain and culture conditions | 第33-34页 |
| ·Growth analysis | 第34-36页 |
| ·Effect of pH on the growth of Chlorella sorokiniana CS-01 | 第36-37页 |
| ·Investigation on dry Weight | 第37-38页 |
| ·Paraffin section and observation of microalgal morphology | 第38-39页 |
| ·Results and discussion | 第39-47页 |
| ·Effect of carbon dioxide on growth of Chlorella | 第39-41页 |
| ·Effect of carbon dioxide on the morphology of Chlorella sorokiniana CS-01 | 第41-42页 |
| ·Effect of pH on cell growth | 第42-44页 |
| ·OD-t curve determination by UV-spectrophotometer | 第44-47页 |
| ·Conclusions | 第47-48页 |
| CHAPTER 3 How does simulated flue gases affect on the lipids accumulation and lipidscomposition of Chlorella sorokinia CS-01? | 第48-60页 |
| ·Introduction | 第49页 |
| ·Materials and methods | 第49-53页 |
| ·Reagents and Equipments | 第49-50页 |
| ·Lipid extraction | 第50-52页 |
| ·the analysis of lipids compositon | 第52-53页 |
| ·Results and discussion | 第53-59页 |
| ·Comparison of the four methods for extracting microalgal lipid | 第53-55页 |
| ·The effect of carbon dioxide concentration on lipids accumulation of Chlorella CS001 | 第55-56页 |
| ·The effect of carbon dioxide concentration on fatty acid composition of Chlorella CS-01 | 第56-59页 |
| ·Conclusions | 第59-60页 |
| CHAPTER 4 Effect of simulated flue gases on the expression of three genes related to CO_2fixation and fatty acid synthesis | 第60-77页 |
| ·Introduction | 第61-62页 |
| ·Materials and methods | 第62-67页 |
| ·Major reagents and equipments | 第62页 |
| ·Real-Time PCR assays | 第62-67页 |
| ·Total RNA extraction and cDNA synthesis | 第62-66页 |
| ·Primers design | 第66页 |
| ·Real-time PCR | 第66-67页 |
| ·Results and discussion | 第67-76页 |
| ·Total RNA extraction and analysis | 第67-69页 |
| ·Extraction of genome DNA and 18SrRNA amplification for building standard curve | 第69-71页 |
| ·Real time PCR | 第71-72页 |
| ·Differential expression of genes detected by RT-PCR | 第72-76页 |
| ·Conclusions | 第76-77页 |
| CHAPTER 5 General Conclusions and Research Prospects | 第77-79页 |
| References | 第79-84页 |
| Acknowledgement | 第84-85页 |
| Research achievements during three year's postgraduate career | 第85-86页 |
| Appendix Statistics analysis of all the dates obtained from the experiments in this study | 第86-93页 |
