| List of Figures | 第1-9页 |
| Abbreviations | 第9-10页 |
| 摘要 | 第10-12页 |
| Abstract | 第12-14页 |
| Introduction | 第14-22页 |
| 1. Hepatic stellate cells in Liver fibrosis | 第14页 |
| 2. Liver fibrosis models in vivo | 第14-16页 |
| 3. TLR4/NF-κB signaling in Liver fibrosis and inflammation | 第16-22页 |
| Chapter 1. The anti-fibrotic effect of betulinic acid is mediated through the inhibition ofNF-KB nuclear protein translocation | 第22-45页 |
| Abstract | 第23-24页 |
| ·Introduction | 第24-26页 |
| ·Materials and Methods | 第26-28页 |
| ·Chemical | 第26页 |
| ·In vivo study | 第26-28页 |
| ·Animals | 第26页 |
| ·Animal treatment | 第26-27页 |
| ·Determination of biochemical parameters | 第27页 |
| ·Assay of hydroxyproline | 第27页 |
| ·Western blot analysis in vivo | 第27页 |
| ·Histopathological and immunohistochemistry examination | 第27-28页 |
| ·In vitro study | 第28页 |
| ·Cell culture | 第28页 |
| ·Cell viability assay | 第28页 |
| ·Western blot analysis in vitro | 第28页 |
| ·Statistical analysis | 第28-29页 |
| ·Results | 第29-43页 |
| ·In vivo experiments | 第29-31页 |
| ·The effects of BA on body weight and liver weight induced by TAA | 第29页 |
| ·The biochemical levels and hydroxyproline content of rats treated with BA for TAA-induced liver fibrosis | 第29-30页 |
| ·Histopathological and immunohistochemical changes in rat livers after TAA treatment | 第30页 |
| ·The anti-fibrosis effects of BA in downregulating the expression of α-SMA and collagen-Ⅰ | 第30-31页 |
| ·In vitro experiment | 第31-43页 |
| ·Effects of BA on cell viability | 第31页 |
| ·The effects of BA on α-SMA, TIMP-1, MMP-13, caspase-8 and caspase-3 expression | 第31页 |
| ·The effects of BA on inhibiting TNF-α induced TLR-4/MyD88 signal, p65 nucleus translocation and p65 phosphorylation in HSCs | 第31-43页 |
| ·Discussion | 第43-45页 |
| Chapter 2.Betulinic acid and Betulin alleviated ethanol-induced fat accumulation viamodulation of TLR4/NF-κB signal pathway | 第45-63页 |
| Abstract | 第46-47页 |
| ·Introduction | 第47-49页 |
| ·Materials and Methods | 第49-52页 |
| ·Reagents | 第49页 |
| ·In vivo study | 第49-50页 |
| ·Animals and treatment | 第49页 |
| ·Determination of biochemical parameters and serum triglyceride levels | 第49-50页 |
| ·Histopathological examination | 第50页 |
| ·In vitro study | 第50-51页 |
| ·Cell cultures | 第50页 |
| ·Influence of BA and BT on ethanol cytotoxicity to HSC-T6 cells | 第50页 |
| ·Cell treatment on HSC-T6 cells with ethanol and BA or BT | 第50-51页 |
| ·Western blotting analysis | 第51页 |
| ·Statistical analysis | 第51-52页 |
| ·Results | 第52-61页 |
| ·In vivo | 第52-53页 |
| ·The effect of BA and BT on serum aminotransferase activities and TG levels | 第52页 |
| ·The effect of BA and BT on histopathological changes induced by ethanol in mice | 第52页 |
| ·The effect of BA and BT on modulating CYP2E1, SREBP-1 and p-STAT3 expression | 第52-53页 |
| ·In vitro | 第53-61页 |
| ·The effect of BA and BT on HSC-T6 cells viability incubation with ethanol | 第53页 |
| ·The inhibition of BA and BT on α-SMA and collagen-1 levels in ethanol activated HSCs | 第53页 |
| ·The effects of BA and BT on inhibiting ethanol induced TLR4/MyD88 signal and p65 nucleus translocation in HSCs | 第53-54页 |
| ·The effect of BA and BT on the expression of p-AMPK and p-STAT3 in ethanol activated HSCs | 第54-61页 |
| ·Discussion | 第61-63页 |
| Conclusions | 第63-64页 |
| References | 第64-73页 |
| Publication | 第73-75页 |
| 致谢 | 第75页 |
