| Chinese abstract | 第1-5页 |
| English abstract | 第5-7页 |
| Table of contents | 第7-9页 |
| Chapter 1 General introduction | 第9-33页 |
| 1 Harmful algal blooms and amnesic shellfish poisoning(ASP) | 第9-13页 |
| 2 Structure and acute toxicity of domoic acid(DA) | 第13-17页 |
| 3 Xenobiotic metabolizing enzyme genes,HSP70 and Na~+/K~+-ATPase gene | 第17-31页 |
| ·The cytochrome P450 enzymes(CYPs) | 第17-21页 |
| ·The cytochrome P450 enzyme family | 第18-20页 |
| ·Regulatory expression and function of CYP1A | 第20-21页 |
| ·Soluble glutathione S-transferase | 第21-24页 |
| ·Crystal structures of GSTs | 第22-23页 |
| ·Regulation of GST gene expression | 第23-24页 |
| ·Heat shock protein 70(HSP70) | 第24-28页 |
| ·Heat shock proteins(HSPs) | 第25-27页 |
| ·Regulation of Heat shock protein 70 expression | 第27-28页 |
| ·Na~+/K~+-ATPase | 第28-31页 |
| ·The subunits ofNa~+/K~+-ATPase | 第28-30页 |
| ·The expression of Na~+/K~+-ATPase | 第30-31页 |
| 4 Objectives of current study | 第31-33页 |
| Chapter 2 Transcriptional responses of xenobiotic metabolizing enzymes,HSP70 and Na~+/K~+-ATPase in the liver of rabbitfish intra-peritoneally injected with amnesic shellfish poisoning toxin | 第33-72页 |
| 1 Introduction | 第33-34页 |
| 2 Materials and methods | 第34-38页 |
| 3 Results | 第38-66页 |
| ·Cloning and analysis of the partial cDNA sequences of rabbitfish CYP1A,GSTA,GSTR,HSP70,ATP1A and ACT gene | 第38-60页 |
| ·Isolation of the total RNA from the liver of rabbitfish | 第38页 |
| ·Amplification of the partial cDNA sequence of the rabbitfish CYP1A gene | 第38-42页 |
| ·Amplification of the partial cDNA sequence of the rabbitfish GSTA gene | 第42-46页 |
| ·Amplification of the partial cDNA sequence of the rabbitfish GSTR gene | 第46-48页 |
| ·Amplification of the partial cDNA sequence of the rabbitfish HSP70 gene | 第48-52页 |
| ·Amplification of the partial cDNA sequence of the rabbitfish ATP1A1 gene | 第52-56页 |
| ·Amplification of the partial cDNA sequence of the rabbitfish ACT gene | 第56-60页 |
| ·Semi-quantification of relative liver CYP1A1 mRNA abundance | 第60-61页 |
| ·Semi-quantification of relative liver GSTA mRNA abundance | 第61-62页 |
| ·Semi-quantification of relative liver GSTR mRNA abundance | 第62-63页 |
| ·Semi-quantification of relative liver HSP70 mRNA abundance | 第63页 |
| ·Semi-quantification of relative liver ATP1A mRNA abundance | 第63-66页 |
| 4 Discussion | 第66-72页 |
| Conclusion | 第72-73页 |
| References | 第73-93页 |
| Appendices Ⅰ | 第93-97页 |
| Appendices Ⅱ | 第97-100页 |
| Acknowledgements | 第100页 |
