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基于DNA环状小分子的DNA和RNA纳米组装技术

Catalogue第5-7页
摘要第7-9页
Abstract第9-11页
Chapter 1 Introduction第12-50页
    1.1 Rolling circle amplification(RCA)第15-20页
    1.2 Rolling circle transcription(RCT)第20-23页
    1.3 DNA Nanotechnology第23-36页
        1.3.1 Property of DNA as a material第23页
        1.3.2 DNA Tiles第23-25页
        1.3.3 Self-assembly of 2D-DNA structures第25-26页
        1.3.4 Self-assembly of 3D-DNA structures第26-28页
        1.3.5 DNA Origami第28-35页
        1.3.6 DNA Nanotubes Assembly第35-36页
    1.4 DNA/RNA Hybrid Nanotechnology第36-40页
        1.4.1 Synergistic self-assembly of RNA and DNA molecules第37-38页
        1.4.2 DNA/RNA origami第38-40页
    1.5 Comparison of DNA and RNA第40-43页
        1.5.1 Comparison of DNA and RNA structures and basic property第40-41页
        1.5.2 Comparison of DNA and RNA Self-assembly第41-43页
    References第43-50页
Chapter 2 Periodical Assembly of Repetitive RNA Sequences Synthesized by Rolling CircleTranscription with Short DNA Staple Strands to RNA/DNA Hybrid Nanowires第50-72页
    2.1 Introduction第50-52页
    2.2 Results and Discussions第52-62页
        2.2.1 Procedure of RCT and Agarose gel electrophoresis of R1 and R2第53-54页
        2.2.2 Preparation of Circular template and the scaffold ssRNA第54-55页
        2.2.3 The formation of RNA/DNA hybrid nanowires R1-1第55-57页
        2.2.4 The formation of RNA/DNA hybrid nanowires R1-2第57-58页
        2.2.5 The formation of RNA/DNA hybrid nanowires R2-3第58-60页
        2.2.6 The formation of RNA/DNA hybrid nanowires R1-4第60-62页
    2.3 Experimental Setion第62-68页
        2.3.1 Material and Methods第62页
        2.3.2. Preparation of circular templates第62页
        2.3.3. Rolling Circle Transcription Reaction第62-63页
        2.3.4 Nucleic acid sequences for preparing circular DNA templates第63页
        2.3.5 Nucleic acid sequences for creating nanowires第63-67页
        2.3.6. Argrose Electrophoresis第67页
        2.3.7. Thermal Annealing to Create RNA/DNA Nanowires第67-68页
        2.3.8. AFM Imaging第68页
    2.4 Conclusion第68-69页
    References第69-72页
Chapter 3 Small Circular DNA Molecules Act as Rigid Motifs to Build DNA Nanotubes第72-100页
    3.1 Introduction第72-74页
    3.2 Results and Discussions第74-81页
        3.2.1 Preparation of circular building-blocks第74-75页
        3.2.2 Designs of NT1 and NT2 DNA Nanotubes and their corresponding AFM images第75-78页
        3.2.3 Designs of NT3 DNA Nanotubes and its corresponding AFM images第78-79页
        3.2.4 Designs of NT4 and NT5 DNA Nanotubes and their corresponding AFM images第79-81页
    3.3 Experimental Section第81-96页
        3.3.1 Materials第81页
        3.3.2 Methods and Instruments第81-82页
        3.3.3 Preparation of circular DNA building-blocks第82页
        3.3.4 Denaturing PAGE electrophoretic analysis of the purified circular DNA building-blocks第82-85页
        3.3.5 Self-Assembly of circular DNA with short DNA staples to create robust Nanotubes第85-87页
        3.3.6 Sequences used in the text第87-93页
        3.3.7 More AFM images of nanotubes of NT1~NT5第93-96页
    3.4 Conclusion第96-97页
    Reference第97-100页
Summary第100-102页
本论文创新点第102-105页
Publications第105-106页
致谢第106-107页

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