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Cryopreservation of Seeds:Dormancy and Water Content in Perspective

摘要第5-7页
ABSTRACT第7-10页
Abbreviations第11-15页
Chapter 1 Introduction第15-38页
    1.1 SEED STORAGE第16-25页
        1.1.1 Types of seeds第17-19页
        1.1.2 Usefulness of discrete classification system第19页
        1.1.3 Seed storage conditions第19-22页
        1.1.4 Selecting and treating seeds for storage第22-24页
        1.1.5 Drying seeds – importance of drying rate第24页
        1.1.6 Cryoprotectants第24-25页
    1.2 FREEZING OF IMBIBED SEEDS IN NATURAL ENVIRONMENT第25-30页
        1.2.1 Freezing avoidance-supercooling第26-29页
        1.2.2 Freezing tolerance by freeze-desiccation第29-30页
    1.3 STATE OF WATER IN SEEDS第30-33页
    1.4 USING ORTHODOX SEEDS AT IMBIBED STATE AS A MODEL FOR RECALCITRANT SPECIES第33页
    1.5 DORMANCY第33-37页
    1.6 OUTLINE OF THE CURRENT PROJECT第37-38页
Chapter 2 Dormancy and cryopreservation of Dodonea viscosa seeds第38-50页
    2.1 INTRDOUCTION第38-39页
    2.2 MATERIALS AND METHODS第39-41页
        2.2.1 Seed collection第39页
        2.2.2 Moisture content measurement第39页
        2.2.3 Germination test第39-40页
        2.2.4 Dormancy breaking第40-41页
        2.2.5 Seed imbibition第41页
        2.2.6 Cryopreservation第41页
        2.2.7 Statistical analysis第41页
    2.3 RESULTS第41-45页
        2.3.1 Germination and moisture content of freshly collected seed第41页
        2.3.2 Seed imbibition第41-42页
        2.3.3 Effect of dry storage on dormancy breaking第42页
        2.3.4 Effect of different temperatures on breaking dormancy第42页
        2.3.5 Importance of hot-water temperature in breaking dormancy第42页
        2.3.6 Cryopreservation of seeds第42-45页
    2.4 DISCUSSION第45-50页
Chapter 3 Dormancy and cryopreservation of Apricot (Prunus armeniaca) seeds fromXinjiang province第50-61页
    3.1 INTRODUCTION第50-51页
    3.2 MATERIALS AND METHODS第51-53页
        3.2.1 Seed materials第51页
        3.2.2 Seed desiccation and moisture content determination第51-52页
        3.2.3 Germination test第52页
        3.2.4. Imbibition of water第52页
        3.2.5 Cold stratification第52页
        3.2.6 Effect of GA3 on dormancy break第52-53页
        3.2.7 Effect of GA3 in endocarp intact seeds第53页
        3.2.8 Cryopreservation of seeds第53页
        3.2.9 Statistical analysis第53页
    3.3 RESULTS第53-57页
        3.3.1 Moisture content of the seeds第53-54页
        3.3.2 Germination of seeds第54页
        3.3.3 Imbibition of water第54页
        3.3.4 Effect of GA3 on dormancy break第54页
        3.3.5 Effect of GA3 in endocarp intact seeds第54页
        3.3.6 Cold stratification第54-57页
        3.3.7 Seed desiccation and germination第57页
        3.3.8 Cryopreservation of seeds第57页
    3.4 DISCUSSION第57-61页
Chapter 4 Cryopreservation of hydrated lettuce (Lactuca sativa L.) seeds第61-72页
    4.1 INTRODUCTION第61-63页
    4.2 MATERIALS AND METHODS第63-65页
        4.2.1 Seed material and germination conditions第63页
        4.2.2 Seed imbibition and moisture content determination第63页
        4.2.3 Thermal analyses第63-64页
        4.2.4 Controlled cooling of seeds第64页
        4.2.5 Liquid nitrogen storage第64页
        4.2.6 Use of pierced seeds第64页
        4.2.7 Statistical analysis第64-65页
    4.3 RESULTS第65-68页
    4.4 DISCUSSION第68-72页
Chapter 5 Effects of dimethyl sulfoxide concentration, pre-cooling and cooling rate oncryopreservation of hydrated lettuce seeds第72-85页
    5.1 INTRODUCTION第72-73页
    5.2 MATERIALS AND METHODS第73-75页
        5.2.1 Seed materials第73-74页
        5.2.2 Seed germination第74页
        5.2.3 Moisture content assessment第74页
        5.2.4 Seed imbibition第74页
        5.2.5 DMSO treatment第74页
        5.2.6. Freezing treatments and differential thermal analysis (DTA)第74-75页
        5.2.7 Statistical analysis第75页
    5.3 RESULTS第75-81页
    5.4. DISCUSSION第81-85页
Chapter 6 Summary of main findings第85-87页
References第87-111页
List of papers published or in progress duringthe post graduate study第111-113页
Acknowledgements第113页

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