| DEDICATION | 第3-6页 |
| ABSTRACTS | 第6-7页 |
| 摘要 | 第8-12页 |
| CHAPTER-1：INTRODUCTION | 第12-14页 |
| CHAPTER-2：REVIEW OF LITERATURE RELATED WITH MUTATION STUDY | 第14-24页 |
| 2.1 The Origin of Cucumber | 第14页 |
| 2.2 Botany and Plant Description | 第14-15页 |
| 2.3 What is Mutation? | 第15页 |
| 2.4 Mutation Breeding | 第15-18页 |
| 2.5 Chemical Mutagens | 第18-19页 |
| 2.6 Ethyl Methanesulfonate (EMS) and Its Reaction with DNA | 第19-20页 |
| 2.7 EMS Dose Optimization and Different Treatment Conditions | 第20页 |
| 2.8 Effect of EMS on Germination and Cotyledon Leaves Development and seedling Growth | 第20-21页 |
| 2.9 Effect of EMS on Chlorophyll Mutation | 第21页 |
| 2.10 Effect of EMS on Plant Survival and Plant Height | 第21-22页 |
| 2.11 Effect of EMS on Leaves Development/Shape | 第22页 |
| 2.12 Effect of EMS on Flower, Fruits and Seeds Production | 第22-24页 |
| CHAPTER-3：DOSE OPTIMIZATION EXPERIMENTS OF EMS | 第24-36页 |
| 3.1 Material and Methods | 第24-26页 |
| 3.1.1 Plant material | 第24页 |
| 3.1.2 EMS Concentration and Post-Treatment | 第24页 |
| 3.1.3 EMS Concentration and Treatment Temperature | 第24页 |
| 3.1.4 EMS Concentration and Treatment Time | 第24-25页 |
| 3.1.5 Germination | 第25-26页 |
| 3.1.6 Design and Analysis | 第26页 |
| 3.2 Results and Discussion | 第26-36页 |
| 3.2.1 Effect EMS and Treatment Conditions on Germination Parameters | 第26-32页 |
| 3.2.2 Effect of EMS and Treatment Time on Seedlings Growth Parameters | 第32-34页 |
| 3.2.3 Dwarf and Tall Seedlings | 第34-36页 |
| CHAPTER-4 ：MUTATION IN CUCUMBER DURING REGULAR EXPERIMENT (M_1GENERATION) IN FALL SEASON, 2013 | 第36-41页 |
| 4.1 Materials and Methods | 第36页 |
| 4.2 Results and Discussion | 第36-41页 |
| 4.2.1 Effect of EMS on Germination Parameters during M1 Generation | 第36-37页 |
| 4.2.2 Important Variations induced by EMS in Cucumber Cultivar Chinese Long (9930) during M1 Generation | 第37-41页 |
| CHAPTER-5：THE STUDY OF INDUCED MUTATION IN CUCUMBER CULTIVAR CHINESE LONG (LINE 9930) IN M1 (SPRING) & M2 (FALL) GENERATIONS DURING 2014 | 第41-71页 |
| 5.1 Material and Methods | 第41-42页 |
| 5.1.1 Plant material | 第41页 |
| 5.1.2 Seed Treatments with EMS | 第41页 |
| 5.1.3 Seeds Sowing after EMS Treatment | 第41页 |
| 5.1.4 Generations | 第41-42页 |
| 5.2 Results | 第42-67页 |
| 5.2.1 M1 Generation | 第42-51页 |
| 5.2.2 M2 Generation | 第51-67页 |
| 5.3 Discussion | 第67-71页 |
| 5.3.1 M1 Generation | 第67-69页 |
| 5.3.2 M2 Generation | 第69-71页 |
| CHAPTER-6： SUMMARY AND CONCLUSION | 第71-74页 |
| SUMMARY | 第71-73页 |
| CONCLUSION | 第73-74页 |
| ACKNOWLEDGEMENT | 第74-75页 |
| LITERATURE CITED | 第75-81页 |
| APPENDIX: PROCEDURE FOR CUCUMBER SEEDS TREATMENT | 第81-83页 |
| Curriculum vitae | 第83-88页 |
