| ABSTRACT | 第5-6页 |
| 摘要 | 第7-11页 |
| CHAPTER Ⅰ: LITERATURE REVIEW | 第11-28页 |
| 1.1 Introduction | 第11-12页 |
| 1.2 Breast cancer | 第12-14页 |
| 1.3 Types of breast cancer | 第14-16页 |
| 1.4 Epidiliology and risk factors | 第16-17页 |
| 1.5 Apoptosis | 第17-19页 |
| 1.6 Reactive oxygen species | 第19-21页 |
| 1.7 Cell cycle progression | 第21-22页 |
| 1.8 Mitochondria menbrane potential (△Ψm) | 第22-25页 |
| 1.9 Western Bloting | 第25-26页 |
| 1.10 Hypothesis of research work | 第26页 |
| 1.11 Objectives of present study | 第26-28页 |
| CHAPTER Ⅱ: NATURAL COMPOUNDS CHEMOPREVENTIVE AGENTS | 第28-34页 |
| 2.1 Medicinal Plants | 第28-29页 |
| 2.2 Alkaloids exert anti-roliferative activity on cancer cells | 第29-31页 |
| 2.3 Costunolide | 第31-32页 |
| 2.4 Molecular targets of costunolide | 第32-34页 |
| CHAPTER Ⅲ: MATERIAL AND METHODS | 第34-38页 |
| 3.1 Chemicals and Reagents | 第34页 |
| 3.2 Cell Culture | 第34页 |
| 3.3 Cell Proliferation Assay (MTT) | 第34-35页 |
| 3.4 Flow Cytometric Analysis of Cell Cycle | 第35页 |
| 3.5 Flow Cytometric Determination of Apoptosis | 第35-36页 |
| 3.6 Flow Cytometric Determination of Reactive Oxygen Species (ROS) | 第36页 |
| 3.7 Flow Cytometric Determination of Mitochondrial Membrane Potential (△Ψm) | 第36页 |
| 3.8 Western Blotting | 第36-37页 |
| 3.9 Statistical Analysis of Data | 第37-38页 |
| CHAPTER Ⅳ: RESULTS AND DISCUSSION | 第38-49页 |
| 4.1 Anti-proliferative effects of Costunolide on breast cancer cells | 第38-39页 |
| 4.2 Costunolide Induced G2/M Cell Cycle Arrest on breast cancer MCF7 Cells | 第39-40页 |
| 4.3 Costunolide Induced Apoptotic Cell Death in Breast Cancer MCF7 Cells | 第40-42页 |
| 4.4 Costunolide Increased Generation of ROS in MCF7 Cells | 第42-43页 |
| 4.5 Costunolide Decreased Mitochondrial Membrane Potential in MCF7 cells | 第43-44页 |
| 4.6 Costunolide Regulated Apoptosis-Related Proteins in MCF-7 Cells | 第44-46页 |
| 4.7 CONCLUSION | 第46页 |
| 4.8 Novel findings and significance | 第46-48页 |
| 4.9 List of abreviations | 第48-49页 |
| References | 第49-68页 |
| ACKNOWLEDGEMENTS | 第68-69页 |
