| 摘要 | 第4-5页 |
| ABSTRACT | 第5-6页 |
| CHAPTER 1 INTRODUCTION | 第9-29页 |
| 1.1 Marine oil pollution | 第9-19页 |
| 1.1.1 Crude oil | 第9页 |
| 1.1.2 Crude oil components | 第9-10页 |
| 1.1.3 Marine oil pollution status quo | 第10-13页 |
| 1.1.4 Marine oil pollution damage | 第13-16页 |
| 1.1.5 The behavior of oil into the sea | 第16-17页 |
| 1.1.6 Marine oil pollution of bioremediation | 第17-19页 |
| 1.2 Ocean petroleum hydrocarbon microbial degradation pathway of speciesdiversity | 第19-26页 |
| 1.2.1 Petroleum hydrocarbons microbial metabolic pathways | 第19-24页 |
| 1.2.2 The research progress of Marine petroleum hydrocarbon degradationmicroorganisms | 第24-26页 |
| 1.3 The research purpose and meaning of this paper | 第26-29页 |
| CHAPTER 2 CRUDE OIL DEGRADING BACTERIA | 第29-39页 |
| 2.1 Isolation of crude oil degrading bacteria | 第29-34页 |
| 2.1.1 Chemicals | 第29-30页 |
| 2.1.2 Equipment | 第30-31页 |
| 2.1.3 Nutrient requirements | 第31-32页 |
| 2.1.4 Analysis of crude oil | 第32页 |
| 2.1.5 Enrichment and isolation of crude oil degrading bacteria | 第32页 |
| 2.1.6 Results | 第32-34页 |
| 2.2 Identification of crude oil degrading bacteria | 第34-39页 |
| 2.2.1 Chemicals | 第34页 |
| 2.2.2 Equipment | 第34-35页 |
| 2.2.3 Methods | 第35-36页 |
| 2.2.4 Results and discussion | 第36-39页 |
| CHAPTER 3 BIODEGRADATION OF CRUDE OIL BY SINGLE STRAINS | 第39-47页 |
| 3.1 Materials | 第39-41页 |
| 3.1.1 Chemicals | 第39-40页 |
| 3.1.2 Equipment | 第40-41页 |
| 3.2 Methods | 第41-43页 |
| 3.3 Results and discussion | 第43-47页 |
| 3.3.1 Degradation of Total Petroleum Hydrocarbon | 第43-45页 |
| 3.3.2 Degradation of alkanes | 第45-47页 |
| CHAPTER 4 CONSTRUCTION OF BACTERIAL CONSORTIUM | 第47-51页 |
| 4.1 Materials | 第47页 |
| 4.2 Methods: Construction of bacterial consortium | 第47页 |
| 4.3 Results and discussion | 第47-51页 |
| CHAPTER 5 NEW PHYSICAL ADSORPTION BIODEGRADATIONMATERIAL PREPARATION | 第51-67页 |
| 5.1 Material preparation | 第51页 |
| 5.2 Experimental Materials and Methods | 第51-56页 |
| 5.2.1 Main experimental apparatus and reagents | 第51-52页 |
| 5.2.2 Preparation of oil-absorbing materials | 第52-53页 |
| 5.2.3 Preparation of porous materials | 第53-54页 |
| 5.2.4 Adsorption degradation experiments | 第54页 |
| 5.2.5 Inorganic salt detection method | 第54-56页 |
| 5.3 Results and discussion | 第56-64页 |
| 5.3.1 Material preparation | 第56-57页 |
| 5.3.2 Inorganic salt concentration and residual oil concentration detection | 第57-59页 |
| 5.3.3 Petroleum degradation results | 第59-63页 |
| 5.3.4 Morphology analysis of porous materials | 第63-64页 |
| 5.4 Chapter Conclusion | 第64-67页 |
| CHAPTER 6 CONCLUSION AND PROSPECT | 第67-69页 |
| REFERENCES | 第69-77页 |
| ACADEMIC PAPERS PUBLISHED DURING THE STUDY FOR A MASTER’SDEGREE | 第77-79页 |
| ACKNOWLEDGEMENT | 第79-80页 |
