| Statement in Chinese | 第1-4页 |
| Contents | 第4-8页 |
| List of Figures | 第8-9页 |
| List of Tables | 第9-10页 |
| List of Abbreviations | 第10-12页 |
| Abstract in Chinese | 第12-15页 |
| Abstract in English | 第15-18页 |
| Chapter 1 Bacillus thuringiensis: from gene to genomics to commercialization | 第18-54页 |
| ·Introduction | 第19页 |
| ·B. thuringiensis research and development | 第19-32页 |
| ·Major events in the progress of B. thuringiens | 第19-21页 |
| ·Key bioactive elements of B. thuringiensis | 第21-25页 |
| ·δ-endotoxins | 第21页 |
| ·β-exotoxin | 第21-22页 |
| ·Chitinase | 第22页 |
| ·Vegetative insecticidal proteins | 第22-23页 |
| ·Zwittermicin A | 第23-24页 |
| ·non-Cyt inclusion proteins against cancer cells | 第24页 |
| ·S-layer proteins | 第24-25页 |
| ·Mode of action | 第25-26页 |
| ·Tridimensional structure of Cry toxins and their functions | 第25页 |
| ·Helper proteins (Molecular chaperons) | 第25-26页 |
| ·Plasmid transfer | 第26页 |
| ·cry gene expression and regulation | 第26-27页 |
| ·Transgenic expression of B. thuringiensis toxin genes | 第27-30页 |
| ·Genetically modified microorganisms | 第27-28页 |
| ·Fermentation study | 第28-29页 |
| ·Transgenic plants | 第29-30页 |
| ·Registered biopesticides based on B. thuringiensis in P R China and U.S | 第30-32页 |
| ·Problems & solutions | 第32-38页 |
| ·Relationship between B. thuringiensis and B. cereus | 第32-33页 |
| ·Safety assessment or B. thuringiensis toxins | 第33-35页 |
| ·Effects on soil | 第33页 |
| ·Effects on water | 第33页 |
| ·Effects on plants | 第33页 |
| ·Effects on non-target microbes and invertebrates | 第33-34页 |
| ·Effects on vertebrates | 第34-35页 |
| ·Effects on food | 第35页 |
| ·Comparative sensitivity to UV radiation | 第35-36页 |
| ·Resistance and insect resistance management | 第36-38页 |
| ·Mechanism of resistance | 第37-38页 |
| ·Insect resistance management | 第38页 |
| ·Progress in B. thuringiensis | 第38-50页 |
| ·Screening and identification of B. thuringiensis strains | 第38-39页 |
| ·Identification of ICPs genes | 第39-40页 |
| ·Toxin receptors | 第40页 |
| ·Quorum-sensing | 第40-41页 |
| ·Secretion of metabolites | 第41-42页 |
| ·B. thuringiensis phages | 第42-43页 |
| ·Functional genomics | 第43-50页 |
| ·Genome sequencing | 第43页 |
| ·Identification of novel functional genes | 第43-46页 |
| ·Transposable elements | 第46-47页 |
| ·Proteomics analysis | 第47-48页 |
| ·Transcriptomics analysis | 第48-50页 |
| ·Conclusion | 第50-51页 |
| ·Rationale and significance | 第51-54页 |
| Chapter 2 PCR-RFLP screening of cry gene profiles in B. thuringiensis and characterization of two novel cry2-type genes | 第54-70页 |
| ·Introduction | 第55-56页 |
| ·Materials and methods | 第56-62页 |
| ·Bacterial strains | 第56页 |
| ·Preparation of plasmid DNA | 第56-57页 |
| ·PCR-RFLP screening of cry genes | 第57页 |
| ·PCR amplification of cry2-type genes | 第57-61页 |
| ·Molecular cloning and sequencing of cry2-type genes | 第61页 |
| ·Data analysis | 第61页 |
| ·B. thuringiensis toxin nomenclature | 第61页 |
| ·Nucleotide sequence accession numbers | 第61-62页 |
| ·Results | 第62-68页 |
| ·Identification of cry genes classes from B. thuringiensis | 第62页 |
| ·Diversity of cry gene combinations of B. thuringiensis isolates | 第62-64页 |
| ·Cloning and sequence analysis of cry2Ab8 | 第64页 |
| ·Cloning and sequence analysis of cry2Ad2 | 第64-68页 |
| ·Discussion | 第68-70页 |
| Chapter 3 Cloning and characterization of aiiA from Bacillus: evidence for the existence of an AHL-lactonase gene in B. subtilis BS-1 | 第70-84页 |
| ·Introduction | 第71-72页 |
| ·Materials and methods | 第72-75页 |
| ·Bacterial strains and culture conditions | 第72页 |
| ·DNA preparation, primers and PCR amplification | 第72-73页 |
| ·Molecular cloning and sequencing | 第73页 |
| ·Sequence analysis | 第73页 |
| ·Three-dimensional structure prediction | 第73页 |
| ·Expression of the recombinant GST-AiiA fusion protein | 第73-74页 |
| ·Biological activity assay of BS-1 and its GST-AiiA fusion protein | 第74-75页 |
| ·Nucleotide sequence accession numbers | 第75页 |
| ·Results | 第75-81页 |
| ·Detection of aiiA in B. thuringiensis, B. cereus and B. subtilis | 第75页 |
| ·Cloning and sequence analysis of the aiiA gene | 第75-78页 |
| ·3-D structure prediction of putative AiiA from BS-1 | 第78页 |
| ·BS-1 and its GST-AiiA fusion protein suppressed the virulence of E, carotovora var. carotovora Dya | 第78-81页 |
| ·Discussion | 第81-84页 |
| Chapter 4 Identification, distribution pattern of IS231 elements in B. thuringiensis and their phylogenetic analysis | 第84-95页 |
| ·Introduction | 第85页 |
| ·Materials and methods | 第85-88页 |
| ·Bacterial strains and plasmids | 第85-87页 |
| ·DNA sample preparation and PCR conditions | 第87页 |
| ·IS231-like elements detection | 第87页 |
| ·Molecular cloning | 第87-88页 |
| ·Data and phylogenetic analysis | 第88页 |
| ·Nucleotide sequence accession numbers | 第88页 |
| ·Results | 第88-93页 |
| ·Occurrence of IS231 elements | 第88-89页 |
| ·Data analysis of novel IS231 | 第89-91页 |
| ·Phylogenetic analysis | 第91-93页 |
| ·Discussion | 第93-95页 |
| Chapter 5 Identification of genes differentially expressed during the development of B. thuringiensis | 第95-109页 |
| ·Introduction | 第96-97页 |
| ·Materials and methods | 第97-100页 |
| ·Bacterial strains and plasmids | 第97页 |
| ·RNA preparation | 第97-98页 |
| ·RAP-PCR | 第98页 |
| ·Isolation, cloning of RAP-PCR fragments | 第98页 |
| ·DNA sequencing | 第98-100页 |
| ·Confirmation of differential gene expression | 第100页 |
| ·Nucleotide sequence accession numbers | 第100页 |
| ·Results | 第100-107页 |
| ·Growth process of B. thuringiensis strain 8010 | 第100-102页 |
| ·RAP-PCR analysis | 第102-104页 |
| ·Verification of differential gene expression by specifically primed RT-PCR | 第104-105页 |
| ·Characterization of the growth-regulated genes | 第105-107页 |
| ·Discussion | 第107-109页 |
| Chapter 6 Concluding remarks | 第109-112页 |
| References | 第112-135页 |
| Appendices | 第135-139页 |
| Acknowledgements | 第139页 |
