| 摘要 | 第6-8页 |
| ABSTRACT | 第8-9页 |
| LIST OF ABBREVIATIONS | 第12-15页 |
| CHAPTER ONE INTRODUCTION | 第15-26页 |
| 1.1 COTTON (GOSSYPIUM SPP.) | 第15-17页 |
| 1.2 MYB FAMILY | 第17-18页 |
| 1.3 EVOLUTION OF MYB DNA BINDING DOMAINS | 第18-19页 |
| 1.4 MYB TRANSCRIPTION FACTORS AND DROUGHT TOLERANCE | 第19-22页 |
| 1.5 GENETIC APPROACHES AND MOLECULAR REGULATION FOR DROUGHT TOLERANCE | 第22-25页 |
| 1.6 HYPOTHESIS | 第25页 |
| 1.7 RESEARCH OBJECTIVES | 第25-26页 |
| CHAPTER TWO FUNCTIONAL CHARACTERIZATION OF COTTON GAMYB62L, A NOVEL R2R3 TFIN TRANSGENIC ARABIDOPSIS | 第26-53页 |
| 2.1 MATERIAL AND METHODS | 第26-30页 |
| 2.1.1 Bioinformatic analysis of GaMYB62L gene | 第26页 |
| 2.1.2 Plant transformation and screening of 35S: GaMYB62L Arabidopsis lines | 第26-27页 |
| 2.1.3 Response of overexpressed GaMYB62L transgenic lines to drought and cold stress | 第27页 |
| 2.1.4 Germination and root elongation assays | 第27-28页 |
| 2.1.5 Measurements of transpirational Water loss rate and Relative water content | 第28页 |
| 2.1.6 Analysis of proline content and chlorophyll contents | 第28-29页 |
| 2.1.7 Stomatal opening rate determination in response to Abscisic acid treatment | 第29页 |
| 2.1.8 Gene expression analysis of Markers genes by qRT-PCR | 第29页 |
| 2.1.9 Statistical analysis of Data | 第29-30页 |
| 2.2 RESULTS | 第30-47页 |
| 2.2.1 GaMYB62L Sequence Characterization and Phylogenetic analysis | 第30-34页 |
| 2.2.2 GaMYB62L overexpression positively modulates drought tolerance in transgenic linesof Arabidopsis | 第34-36页 |
| 2.2.3 Analysis of proline and chlorophyll content in GaMYB62L transgenic lines | 第36页 |
| 2.2.4 Constitutive expression of 35S:GaMYB62L in Arabidopsis up-regulates tolerance toABA, salt and osmotic stresses | 第36-42页 |
| 2.2.5 Reduced leaf Stomatal Density, size and stomatal opening in 35S:GaMYB62L | 第42-44页 |
| 2.2.6 Cold response of GaMYB62L-overexpressing Arabidopsis plants | 第44页 |
| 2.2.7 Transcripts investigation of abiotic stress-responsive genes | 第44-47页 |
| 2.3 DISCUSSION | 第47-53页 |
| CHAPTER THREE GAMYB85, NOVEL COTTON R2R3 TF TRANSFORMED IN ARABIDOPSIS HASIMPORTANT ROLE IN DROUGHT TOLERANCE | 第53-80页 |
| 3.1 MATERIAL AND METHODS | 第53-57页 |
| 3.1.1 Bioinformatic analysis of GaMYB85 gene | 第53页 |
| 3.1.2 Transgenic plant construction and screening | 第53-54页 |
| 3.1.3 Germination and root elongation assays | 第54页 |
| 3.1.4 Drought and freezing tolerance assay | 第54-55页 |
| 3.1.5 Measurements of transpirational WLR and RWC | 第55页 |
| 3.1.6 Proline content and chlorophyll content | 第55-56页 |
| 3.1.7 Stomata measurements and opening rates determination in response to Abscisic acidtreatment | 第56页 |
| 3.1.8 Gene expression analysis of marker genes by qRT-PCR | 第56-57页 |
| 3.1.9 Statistical analysis of Data | 第57页 |
| 3.2 RESULTS | 第57-74页 |
| 3.2.1 GaMYB85 characterization and phylogenetic analysis | 第57-60页 |
| 3.2.2 Overexpression of 35S:GaMYB85 in plants confers ABA hypersensitivity for seedgermination and root elongation | 第60-64页 |
| 3.2.3 Overexpression of 35S:GaMYB85 in plants confers enhanced salt and osmotictolerance | 第64-68页 |
| 3.2.4 Enhanced GaMYB85 tolerance under drought and freezing stress | 第68-71页 |
| 3.2.5 Reduced stomatal density, increased stomatal response, and increased stomata sizein GaMYB85 plants | 第71-73页 |
| 3.2.6 Abiotic stress responsive marker genes transcript analysis | 第73-74页 |
| 3.3 DISCUSSION | 第74-80页 |
| CHAPTER FOUR CONCLUSION | 第80-82页 |
| References | 第82-96页 |
| SUPPLEMENTARY DATA I | 第96-105页 |
| SUPPLEMENTARY DATA II | 第105-111页 |
| ACKNOWLEDGEMENT | 第111-113页 |
| RESUME | 第113-114页 |
