| 摘要 | 第1-16页 |
| LIST OF TABLES | 第16-18页 |
| LIST OF FIGURES | 第18-19页 |
| ABSTRACT | 第19-22页 |
| LIST OF ABBREVIATIONS | 第22-24页 |
| CHAPTER 1 LITERATURE REVIEW AND OBJECTIVES OF THE STUDY | 第24-36页 |
| ·GLOBAL CLIMATE CHANGE, POPULATION INCREASE AND FOOD PRODUCTION | 第24-25页 |
| ·ACID SOILS: FORMATION AND GLOBAL DISTRIBUTION | 第25-26页 |
| ·ALUMINUM PHYTOTOXICITY MECHANISM | 第26页 |
| ·ALUMINUM TOLERANCE MECHANISMS | 第26-27页 |
| ·SCREENING METHODS FOR ALUMINUM TOLERANCE | 第27-29页 |
| ·GENETIC ANALYSIS OF ALUMINUM TOLERANCE | 第29-30页 |
| ·MOLECULAR MARKERS | 第30-31页 |
| ·QUANTITATIVE TRAIT LOCI (QTL) ANALYSIS | 第31-33页 |
| ·ASSOCIATION MAPPING | 第33-35页 |
| ·OBJECTIVES OF THE STUDY | 第35-36页 |
| CHAPTER 2 EVALUATION PROCEDURE AND INDICATOR OF TOLERANCE TO ALUMINUM TOXICITY AT SEEDLING STAGE UNDER SAND CULTURE | 第36-46页 |
| ·INTRODUCTION | 第36-38页 |
| ·MATERIALS AND METHODS | 第38-40页 |
| ·Plant materials | 第38-39页 |
| ·Experiment design | 第39-40页 |
| ·Measurements and data analysis | 第40页 |
| ·RESULTS | 第40-44页 |
| ·Selecting suitable Al indicator | 第40-42页 |
| ·Selecting suitable stage for screening using RTDW | 第42页 |
| ·Selecting elite materials for Al tolerance | 第42-44页 |
| ·DISCUSSION | 第44-46页 |
| ·RTDW as the Al tolerant indicator | 第44-45页 |
| ·Appropriate stage for screening for tolerance to Al toxicity | 第45-46页 |
| CHAPTER 3 INHERITANCE AND QTL ANALYSIS OF TOLERANCE TO ALUMINUM TOXICITY AT SEEDLING STAGE UNDER SAND CULTURE | 第46-76页 |
| ·INTRODUCTION | 第46-48页 |
| ·MATERIALS AND METHODS | 第48-54页 |
| ·Materials | 第48-49页 |
| ·Experiment carried out | 第49-50页 |
| ·Statistical analysis | 第50页 |
| ·Method of segregation analysis | 第50-52页 |
| ·DNA extraction and PCR | 第52页 |
| ·Construction of genetic linkage map | 第52-53页 |
| ·QTL mapping | 第53-54页 |
| ·RESULTS | 第54-69页 |
| ·Frequency distribution of Al tolerance in the population | 第54-56页 |
| ·Selection of inheritance model fitting Al tolerance | 第56-59页 |
| ·Estimates of genetic parameters | 第59-60页 |
| ·Genetic constitution ofAl tolerance | 第60-61页 |
| ·Additive effect QTLs | 第61-63页 |
| ·Epistatic effect QTLs | 第63-66页 |
| ·Main QTLs for RTDW and QTL x E interactions | 第66-67页 |
| ·Summary of detected QTLs | 第67-69页 |
| ·DISCUSSION | 第69-76页 |
| ·Traits and genetic model for Al tolerance | 第69-70页 |
| ·Genetic constitution ofAl tolerance | 第70页 |
| ·Additive QTLs and QTL x Environment interactions | 第70-71页 |
| ·Epistatic QTL pairs | 第71-72页 |
| ·Comparisons with previous studies and functions of the delected QTLs | 第72-73页 |
| ·Breeding implications | 第73-76页 |
| CHAPTER 4 INHERITANCE AND QTL ANALYSIS OF TOLERANCE TO ALUMINUM TOXICITY AT SEEDLING STAGE UNDER HYDROPONICS CULTURE | 第76-92页 |
| ·INTRODUCTION | 第76-77页 |
| ·MATERIALS AND METHODS | 第77-80页 |
| ·Materials | 第77-78页 |
| ·Methods | 第78-79页 |
| ·DNA extraction and PCR | 第79页 |
| ·Genetic segregation analysis | 第79页 |
| ·Statistical analysis | 第79-80页 |
| ·Construction of genetic linkage map | 第80页 |
| ·QTL mapping | 第80页 |
| ·RESULTS | 第80-87页 |
| ·Frequency distribution ofRRE | 第80-81页 |
| ·Inheritance model fitting Al tolerance | 第81-83页 |
| ·Genetic constitution of Al tolerance | 第83-84页 |
| ·Additive effect QTLs | 第84-86页 |
| ·Epistatic effect QTLs | 第86页 |
| ·Summary of detected QTLs | 第86-87页 |
| ·DISCUSSION | 第87-92页 |
| ·Scoring index for Al tolerance | 第87页 |
| ·Genetic model for Al tolerance with RRE | 第87-88页 |
| ·The genetic system ofAl tolerance | 第88页 |
| ·QTL comparisons and QTL functions | 第88-90页 |
| ·Conclusions | 第90-92页 |
| CHAPTER 5 ASSOCIATION ANALYSIS OF TOLERANCE TO ALUMINUM TOXICITY IN CHINESE SOYBEAN RELEASED CULTIVARS | 第92-122页 |
| ·INTRODUCTION | 第92-95页 |
| ·MATERIALS AND METHODS | 第95-103页 |
| ·Materials | 第95-97页 |
| ·Phenotyping | 第97-98页 |
| ·DNA extraction | 第98页 |
| ·SSR markers and genome-wide scan | 第98-101页 |
| ·Statistical analysis | 第101-103页 |
| ·RESULTS | 第103-116页 |
| ·Phenotypic analysis of RRE | 第103-104页 |
| ·SSR marker polymorphism | 第104页 |
| ·Linkage disequilibrium | 第104-106页 |
| ·Population structure | 第106-108页 |
| ·QTLs or markers associated with Al tolerance | 第108-109页 |
| ·Exploration of superior Al tolerance alleles | 第109-110页 |
| ·Allele constitutions of elite cultivars tolerant to Al toxin | 第110-112页 |
| ·Tracing the elite alleles in five pedigrees of the released cultivars | 第112-116页 |
| ·DISCUSSION | 第116-122页 |
| ·The rationality and effectiveness of the tested population in association mapping ofAl tolerance QTLs | 第116-117页 |
| ·Importance and implications of linkage disequilibrium and population structure | 第117-118页 |
| ·Association mapping combined with linkageship mapping in finding Al tolerance loci and alleles | 第118-120页 |
| ·Implications to Al tolerance breeding | 第120-122页 |
| CHAPTER 6 OVERALL DISCUSSION AND CONCLUSIONS | 第122-128页 |
| ·OVERALL DISCUSSION | 第122-127页 |
| ·Usefulness of different evaluation procedures and indicators and our choices for Al tolerance breeding programs | 第122-123页 |
| ·Genetic constitution ofAl tolerance | 第123-124页 |
| ·Association mapping combined with linkageship mapping provides a way to genotype the materials, detect elite alleles and make breeding by design practicable | 第124-126页 |
| ·Elite accessions screened out, their QTL constitutions and their potential in future Al tolerance improvement | 第126-127页 |
| ·CONCLUSIONS AND MAJOR FINDINGS | 第127-128页 |
| REFERENCES | 第128-140页 |
| PUBLICATIONS | 第140-142页 |
| ACKNOWLEDGEMENTS | 第142页 |
| BIOGRAPHICAL DATA | 第142页 |
| DEDICATION | 第142页 |
