| Chinese abstract | 第1-6页 |
| English abstract | 第6-9页 |
| Table of contents | 第9-11页 |
| Chapter 1 General introduction | 第11-22页 |
| 1 Leptin in mammals | 第11-16页 |
| 2 Leptin in amphibians | 第16-18页 |
| 3 Leptin in teleosts | 第18-20页 |
| 4 Objectives of current study | 第20-22页 |
| Chapter 2 Gene structure of grass carp leptin | 第22-38页 |
| 1 Introduction | 第22页 |
| 2 Materials and methods | 第22-25页 |
| 3 Results | 第25-35页 |
| ·Isolation and analysis of the full-lenth cDNA sequence of grass carp leptin gene | 第25-28页 |
| ·Cloning and characterization of the genomic structure of grass carp leptin gene | 第28-35页 |
| 4 Discussion | 第35-38页 |
| Chapter 3 Recombinant expression of grass carp leptin | 第38-50页 |
| 1 Introduction | 第38-39页 |
| 2 Materials and methods | 第39-42页 |
| 3 Results | 第42-46页 |
| ·Construction of the reconbinant expression vector of grass carp leptin | 第42-43页 |
| ·Induction and purification of reconbinant grass carp leptin | 第43-44页 |
| ·Characterization of reconbinant grass carp leptin | 第44-46页 |
| 4 Discussion | 第46-50页 |
| Chapter 4 Functional characterization of grass carp leptin | 第50-75页 |
| 1 Introduction | 第50-51页 |
| 2 Materials and methods | 第51-61页 |
| 3 Results | 第61-71页 |
| ·Molecular cloning of key genes correlating to food intake, digestion, energy expenditure and lipid metabolism | 第61-67页 |
| ·Effects of chronic I.P. administration of reconbinant grass carp leptin on food intake and bodyweight | 第67-69页 |
| ·Acute and chronic effects I.P. administration of of rgc-LEP on the expression of selected genesweight | 第69-71页 |
| 4 Discussion | 第71-75页 |
| References | 第75-85页 |
| Appendices | 第85-90页 |
| Acknowledgements | 第90-91页 |
| Papers during the course | 第91-92页 |
