| ABBREVIATIONS | 第8-11页 |
| ABSTRACT | 第11-14页 |
| 摘要 | 第15-22页 |
| CHAPTER 1 INTRODUCTION | 第22-33页 |
| 1.1 Gerbera | 第23-24页 |
| 1.2 Effects of light on plant growth | 第24-33页 |
| 1.2.1 Light emitting diodes | 第24-27页 |
| 1.2.2 Photomorphogenesis | 第27页 |
| 1.2.3 Photoreceptor | 第27-33页 |
| CHAPTER 2 EFFECTS OF LED LIGHT ON IN VITRO CULTURE OF GERBERA | 第33-53页 |
| 2.1 Effects of LED light on Callus induction of gerbera | 第33-41页 |
| 2.1.1 Materials and Methods | 第33-34页 |
| 2.1.2 Results | 第34-41页 |
| 2.2 Effects of LED light on shoot proliferation of gerbera plantlets | 第41-46页 |
| 2.2.1 Materials and Methods | 第41-42页 |
| 2.2.2 Results | 第42-45页 |
| 2.2.3 Discussion | 第45-46页 |
| 2.3 Effects of LED light on root formation of gerbera plantlets | 第46-53页 |
| 2.3.1 Materials and Methods | 第46-47页 |
| 2.3.2 Results | 第47-51页 |
| 2.3.3 Discussion | 第51-53页 |
| CHAPTER 3 EFFECTS OF LED LIGHT ON PHYSIOLOGICAL ANDBIOCHEMICAL CHANGES OF GERBERA PLANTLETS | 第53-68页 |
| 3.1 Materials and Methods | 第53-55页 |
| 3.1.1 Culture medium and conditions | 第53-54页 |
| 3.1.2 Chlorophyll fluorescence | 第54页 |
| 3.1.3 Chlorophyll content | 第54页 |
| 3.1.4 Stomatal density | 第54-55页 |
| 3.1.5 Soluble sugar contents | 第55页 |
| 3.2 Results | 第55-64页 |
| 3.2.1 Effects of LED on chlorophyll fluorescence of gerbera plantlets | 第55-58页 |
| 3.2.2 Effects of LED on chlorophyll content of gerbera plantlets | 第58-60页 |
| 3.2.3 Effects of LED on stomatal density of gerbera plantlets | 第60-62页 |
| 3.2.4 Effects of LED on soluble sugars of gerbera plantlets | 第62-64页 |
| 3.3 Discussion | 第64-68页 |
| 3.3.1 Effects of LED on chlorophyll fluorescence of gerbera plantlets | 第64-65页 |
| 3.3.2 Effects of LED on chlorophyll content of gerbera plantlets | 第65-66页 |
| 3.3.3 Effects of LED on stomatal density of gerbera plantlets | 第66-67页 |
| 3.3.4 Effects of LED on soluble sugars of gerbera plantlets | 第67-68页 |
| CHAPTER 4 GENE CLONING AND BIOINFORMATICS ANALYSIS OF LIGHTRESPONSIVE GENES IN GERBERA | 第68-93页 |
| 4.1 Materials and Methods | 第68-74页 |
| 4.1.1 Designing primers | 第69-70页 |
| 4.1.2 RNA isolation and cDNA synthesis | 第70-71页 |
| 4.1.3 Primer optimization using PCR | 第71-72页 |
| 4.1.4 Gel electrophoresis | 第72-73页 |
| 4.1.5 Gel extraction | 第73页 |
| 4.1.6 TA Cloning | 第73-74页 |
| 4.2 RESULTS | 第74-92页 |
| 4.2.1 PCR cloning | 第74-75页 |
| 4.2.2 Analysis of CRY1, CRY2 and PHYA in transcription data | 第75-76页 |
| 4.2.3 Homologous analysis of CRY1,CRY2 and PHYA | 第76-79页 |
| 4.2.4 Phylogenetic analysis | 第79-80页 |
| 4.2.5 The basic physical and chemical properties of protein analysis | 第80-81页 |
| 4.2.6 Protein hydrophobicity/hydrophilicity prediction and analysis | 第81-82页 |
| 4.2.7 Protein signal peptide prediction analysis | 第82-84页 |
| 4.2.8 Protein subcellular localization analysis | 第84页 |
| 4.2.9 Protein transmembrane structure analysis | 第84-86页 |
| 4.2.10 Protein conserved domain prediction | 第86-87页 |
| 4.2.11 Analysis of coiled coil structure of protein | 第87-88页 |
| 4.2.12 Protein secondary structure prediction | 第88-90页 |
| 4.2.13 Protein three-dimensional structure prediction | 第90-92页 |
| 4.3 Discussion | 第92-93页 |
| CHAPTER 5 EXPRESSION OF LIGHT RESPONSIVE GENES IN GERBERA | 第93-103页 |
| 5.1 Materials and Methods | 第93-96页 |
| 5.1.1 Designing primers | 第93-94页 |
| 5.1.2 RNA isolation | 第94页 |
| 5.1.3 RNA extraction and reverse transcription | 第94-95页 |
| 5.1.4 Quantitative Polymerase Chain Reaction (qPCR) analysis | 第95-96页 |
| 5.2 RESULTS | 第96-101页 |
| 5.2.1 Gene expression analysis of CRY1 and CRY2 using quantitative PolymeraseChain Reaction | 第96-98页 |
| 5.2.2 Effects of LED on the expression of PHYA, CRY1 and CRY2 | 第98-101页 |
| 5.3 Discussion | 第101-103页 |
| CHAPTER 6 CONCLUSION AND OUTLOOK | 第103-105页 |
| REFERENCES | 第105-116页 |
| APPENDIX | 第116-122页 |
| PAPER PUBLICATION | 第122-123页 |
| ACKNOWLEDGEMENTS | 第123页 |
