| 摘要 | 第1-10页 |
| ABSTRACT | 第10-14页 |
| Abbreviations | 第14-17页 |
| 1 Introduction | 第17-22页 |
| 2 Materials and Methods | 第22-39页 |
| ·Materials | 第22-29页 |
| ·Reagents and machines | 第22-24页 |
| ·Cell culture and drug treatment | 第24-28页 |
| ·The list of culture cell lines | 第24页 |
| ·Drug treatment | 第24-28页 |
| ·Reagents for treatment | 第24页 |
| ·Transfection of siRNAS | 第24-28页 |
| ·Clinical samples | 第28-29页 |
| ·Methods | 第29-39页 |
| ·Total RNA extrection | 第29页 |
| ·Reverse Transcription-Polymerase Chain Reaction (RT-PCR) analysis | 第29-31页 |
| ·AFFX microRNA Gene Chip Assay (Chip) | 第31-33页 |
| ·Real-timen PCR(qPCR) | 第33-34页 |
| ·Cell Proliferation analysis (Using MTT assay) | 第34页 |
| ·Sof agar colony transformation | 第34-35页 |
| ·Single cell cloning efficiency | 第35页 |
| ·Migration assay (Scratch assay) | 第35-36页 |
| ·Apoptosis assay (Using FACS) | 第36页 |
| ·Immunofluorescence staining for Ezrin protein in cultured cells | 第36-37页 |
| ·Protein isolation and Western blot analysis | 第37页 |
| ·Tissue microarray construction and immunohistochemical staining | 第37-38页 |
| ·Statistical Analysis | 第38-39页 |
| 3 Results | 第39-56页 |
| ·Differential miRNA expression map in epithelial tumorigenesis by gene Chip | 第39-40页 |
| ·p12-LOX and NF-κB upregulate the mRNA expression level of Ezrin in epithelialcancer cells | 第40-43页 |
| ·p12-LOX mRNA expression in cell lines | 第40-41页 |
| ·Ezrin mRNA expression in cell lines | 第41-42页 |
| ·Ezrin protien expression in gastric cell lines | 第42-43页 |
| ·Ezrin mRNA expression in BAI and BAY trated gastrci cell lines | 第43页 |
| ·p12-LOX and NF-κB inhibitors influence the proliferation ability of B16 and gastriccancers in vitro | 第43-45页 |
| ·Ezrin,p12-LOX and NF-κB could promote the migration of B16 and gastric cancersin vitro | 第45-47页 |
| ·Migration assay and immunofluorescence staing for Ezrin in B16 cells | 第45页 |
| ·Migration assay and immunofluorescence staing for Ezrin in gastric cancer cells | 第45-47页 |
| ·Subcellular localization of Ezrin protein in gastric cancer cells byimmunofluorescence staining | 第47-48页 |
| ·p12-LOX inhibitor could suppress the colony formation of B16 in soft agar | 第48页 |
| ·p12-LOX inhibitor could suppress single cell cloning efficiency | 第48-49页 |
| ·The inhibitors of Ezrin,p12-LOX and NF-κB signaling pathways could induce apoptosis of cancer cells in vitro | 第49-50页 |
| ·Immunohistochemical staining in gastric cancer tissue microarray | 第50-56页 |
| ·Ezrin protein expression in gastric cancers and its precancerous disease | 第50-51页 |
| ·Correlation between Ezrin protein overexpression and clinical parameters of gastric cancers | 第51-56页 |
| 4 Discussion | 第56-62页 |
| 5 Conclusions | 第62-63页 |
| Reference | 第63-72页 |
| 综述 | 第72-93页 |
| 参考文献 | 第84-93页 |
| Appendix Researches in the Period of Doctor's Degree | 第93-95页 |
| 致谢 | 第95页 |
