| 摘要 | 第5-7页 |
| Abstract | 第7-8页 |
| Abbreviation | 第11-12页 |
| INTRODUCTION | 第12-15页 |
| 1.1 Objective | 第13页 |
| 1.2 Hypothesis and specific aim | 第13页 |
| 1.3 Rationale for study | 第13-15页 |
| LITERATURE REVIEW | 第15-24页 |
| 2.1 Epidemiology of esophageal cancer | 第15-17页 |
| 2.2 Incidence and mortality of esophageal cancer in china | 第17页 |
| 2.3 Risk factors of esophageal cancer | 第17-22页 |
| 2.4 Epigenetics processes | 第22-24页 |
| MATERIAL AND METHODOLOGY | 第24-37页 |
| 3.1 Background | 第24页 |
| 3.2 Study Population | 第24-25页 |
| 3.3 Data Collection | 第25页 |
| 3.4 Key Instruments | 第25-26页 |
| 3.5 Extraction of RNA from esophageal carcinoma and paracancerous tissue | 第26页 |
| 3.6 Purification of total RNA | 第26-27页 |
| 3.7 Reverse transcription of RNA | 第27-28页 |
| 3.8 Quantitative real-time PCR | 第28-30页 |
| 3.9 DNA Extraction of Esophageal Cancer and Paracancerous Tissues | 第30-31页 |
| 3.10 Modification and purification of genomic DNA by bisulfite | 第31-33页 |
| 3.11 nest-methylation-specific PCR,MSP | 第33-35页 |
| 3.12 Statistical Analysis | 第35-37页 |
| RESULT | 第37-44页 |
| 4.1 Case characteristics and genotype frequencies | 第37-39页 |
| 4.2 Primers for real-time fluorescence quantitative analysis of DNA repair enzyme gene and PCR product specific analysis of each repair enzyme gene amplification of cDNA samples | 第39-40页 |
| 4.3 Primer sequences for methylation-specific PCR analysis | 第40-41页 |
| 4.4 Correlation analysis of methylation status of MGMT of blood and cancer tissue in esophageal cancer | 第41-42页 |
| 4.5 The correlation between gene expression and methylation status | 第42页 |
| 4.6 The association of MGMT hypermethylation with the risk for EC | 第42-43页 |
| 4.7 Gene-environment interaction | 第43-44页 |
| DISCCUSION CONCLUSION AND RECOMMENDATION | 第44-47页 |
| 5.1 Discussion | 第44-46页 |
| 5.2 Conclusion & Recommendation | 第46-47页 |
| References | 第47-51页 |
| Biography | 第51-52页 |
| Acknowledgements | 第52-53页 |
| Appendix: 1 Dedication | 第53-54页 |
