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食管癌MGMT甲基化及其与环境因素的交互作用研究

摘要第5-7页
Abstract第7-8页
Abbreviation第11-12页
INTRODUCTION第12-15页
    1.1 Objective第13页
    1.2 Hypothesis and specific aim第13页
    1.3 Rationale for study第13-15页
LITERATURE REVIEW第15-24页
    2.1 Epidemiology of esophageal cancer第15-17页
    2.2 Incidence and mortality of esophageal cancer in china第17页
    2.3 Risk factors of esophageal cancer第17-22页
    2.4 Epigenetics processes第22-24页
MATERIAL AND METHODOLOGY第24-37页
    3.1 Background第24页
    3.2 Study Population第24-25页
    3.3 Data Collection第25页
    3.4 Key Instruments第25-26页
    3.5 Extraction of RNA from esophageal carcinoma and paracancerous tissue第26页
    3.6 Purification of total RNA第26-27页
    3.7 Reverse transcription of RNA第27-28页
    3.8 Quantitative real-time PCR第28-30页
    3.9 DNA Extraction of Esophageal Cancer and Paracancerous Tissues第30-31页
    3.10 Modification and purification of genomic DNA by bisulfite第31-33页
    3.11 nest-methylation-specific PCR,MSP第33-35页
    3.12 Statistical Analysis第35-37页
RESULT第37-44页
    4.1 Case characteristics and genotype frequencies第37-39页
    4.2 Primers for real-time fluorescence quantitative analysis of DNA repair enzyme gene and PCR product specific analysis of each repair enzyme gene amplification of cDNA samples第39-40页
    4.3 Primer sequences for methylation-specific PCR analysis第40-41页
    4.4 Correlation analysis of methylation status of MGMT of blood and cancer tissue in esophageal cancer第41-42页
    4.5 The correlation between gene expression and methylation status第42页
    4.6 The association of MGMT hypermethylation with the risk for EC第42-43页
    4.7 Gene-environment interaction第43-44页
DISCCUSION CONCLUSION AND RECOMMENDATION第44-47页
    5.1 Discussion第44-46页
    5.2 Conclusion & Recommendation第46-47页
References第47-51页
Biography第51-52页
Acknowledgements第52-53页
Appendix: 1 Dedication第53-54页

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