| 摘要 | 第7-9页 |
| Abstract | 第9-10页 |
| Abbreviations | 第15-16页 |
| Chapter 1:Literature Review | 第16-30页 |
| 1.1 Poultry production in China | 第16页 |
| 1.2 Indigenous Blue-shelled chicken breeds in China | 第16-17页 |
| 1.3 Egg consumption in the world and China | 第17页 |
| 1.4 The color of eggshell and consumer preferences | 第17-19页 |
| 1.5 Blue eggshell pigmentation | 第19-21页 |
| 1.6 Genes associated with blue eggshell pigmentation | 第21-22页 |
| 1.7 SLCO1B3 gene and EAV-HP insertion in blue-shelled chickens | 第22-23页 |
| 1.8 Genome-wide association and the follow-up replication studies | 第23-24页 |
| 1.9 Overview of Transcriptome-RNA-Seq studies | 第24-25页 |
| 1.10 Introduction to RNA-Seq Technology | 第25-26页 |
| 1.11 Application of RNA-Seq in livestock | 第26-27页 |
| 1.12 Aim and significance of the study | 第27-29页 |
| 1.13 Schematic representation of the workflow | 第29-30页 |
| CHAPTER 2:Analysis On Eggshell Pigments and Detection of mRNA Expression Levelsfor Some Related Major Genes | 第30-52页 |
| 2.1 Introduction | 第30-31页 |
| 2.2 Materials and methods | 第31-42页 |
| 2.3 Results | 第42-48页 |
| 2.4 Discussion | 第48-52页 |
| CHAPTER 3:A Post-GWAS Replication Studies Revealed A Promising Genomic Regionand Candidate Genes for Chicken Eggshell Blueness | 第52-84页 |
| 3.1 Introduction | 第52-53页 |
| 3.2 Materials and Methods | 第53-59页 |
| 3.3 Results | 第59-80页 |
| 3.4 Discussion | 第80-84页 |
| CHAPTER 4 Transcriptomic Profile of Eggshell Gland from Dark and Light Blue Eggshell | 第84-99页 |
| 4.1 Introduction | 第84页 |
| 4.2 Materials and methods | 第84-88页 |
| 4.3 Results | 第88-96页 |
| 4.4 Discussion | 第96-99页 |
| CHAPTER 5: Conclusions | 第99-100页 |
| 5.1 Standard pigment and expression analysis | 第99页 |
| 5.2 Replication association study | 第99页 |
| 5.3 Transcriptome analysis | 第99-100页 |
| References | 第100-114页 |
| Acknowledgments | 第114-116页 |
| Appendices | 第116-126页 |
