| 摘要 | 第9-12页 |
| ABSTRACT | 第12-14页 |
| LIST OF ABBREVIATIONS | 第15-16页 |
| 1 LITERATURE REVIEW | 第16-32页 |
| 1.1 GENUS PSEUDOMONAS | 第16-17页 |
| 1.1.1 Pseudomonas syringae | 第17页 |
| 1.2 IMPORTANCE OF IRON TO LIFE | 第17-21页 |
| 1.2.1 Problems with iron | 第18-19页 |
| 1.2.2 Iron uptake system of Gram-negative bacteria | 第19-21页 |
| 1.3 SIDEROPHORES | 第21-26页 |
| 1.3.1 Types of siderophores | 第21-23页 |
| 1.3.2 Siderophores of Pseudomonas species | 第23-24页 |
| 1.3.3 Pyoverdine | 第24-25页 |
| 1.3.4 Global pathway of a pyoverdine from biosynthesis to secretion in P. aeruginosa | 第25-26页 |
| 1.4 HOST RANGE OF SOME SPECIES OF GENUS PSEUDOMONAS | 第26-27页 |
| 1.5 CAENORHABDITIS ELEGANS | 第27-32页 |
| 1.5.1 C. elegans is a nematode-a member of phylum Nematoda | 第27页 |
| 1.5.2 C. elegans as a model host for studying host-pathogen interaction | 第27-28页 |
| 1.5.3 Survival assays using C. elegans | 第28-30页 |
| 1.5.4 Role of pyoverdine in virulence of bacteria | 第30-32页 |
| 2 AIMS AND SIGNIFICANCE OF THIS STUDY | 第32-34页 |
| 3 MATERIAL AND METHODS | 第34-47页 |
| 3.1 EXPERIMENTAL DESIGN | 第34页 |
| 3.2 BACTERIAL AND C. ELEGANS STRAINS, GENES AND PLASMIDS | 第34-35页 |
| 3.3 APPARATUS AND EQUIPMENT | 第35页 |
| 3.4 CHEMICALS, MEDIA AND GROWTH CONDITIONS | 第35-37页 |
| 3.5 PREPARATION OF BACTERIAL CULTURE | 第37-38页 |
| 3.5.1 Revival of Bacterial Culture | 第37-38页 |
| 3.5.2 Preparation of overnight Bacterial culture | 第38页 |
| 3.5.3 Long-term storage of bacterial culture | 第38页 |
| 3.6 BIOINFORMATICS AND COMPUTER PROGRAMS | 第38页 |
| 3.7 PYOVERDINE DETERMINATION ASSAY | 第38-39页 |
| 3.8 MOLECULAR BIOLOGY TECHNIQUES | 第39-44页 |
| 3.8.1 Polymerase chain reaction (PCR) | 第39-40页 |
| 3.8.2 Agarose gel electrophoresis | 第40页 |
| 3.8.3 Purification of DNA | 第40页 |
| 3.8.4 Plasmid Extraction | 第40-41页 |
| 3.8.5 Preparation of electro-competent cells of P. syringae MB03 | 第41页 |
| 3.8.6 Plasmid DNA transformation into P. syringae MB03 electro-competent cells | 第41-42页 |
| 3.8.7 Disruption of pyoverdine biosynthetic genes by RecTE recombination system | 第42-44页 |
| 3.9 LC-MS ANALYSIS OF CELL FILTRATES OF MB03 AND ITS DERIVATIVES | 第44-45页 |
| 3.10 C. ELEGANS SPECIAL TECHNIQUES | 第45-47页 |
| 3.10.1 Maintenance and Growth Conditions for C. elegans | 第45页 |
| 3.10.2 Synchronization of C. elegans | 第45-46页 |
| 3.10.3 Liquid Killing Assay | 第46-47页 |
| 4 RESULTS | 第47-58页 |
| 4.1. P. SYRINGAE MB03 HAS GENETIC POTENTIAL TO PRODUCE PYOVERDINE | 第47-51页 |
| 4.2. PYOVERDINE IS PRODUCED BY P. SYRINGAE MB03 ONLY UNDER IRON LIMITING CONDITIONS | 第51-52页 |
| 4.3. GENETIC, BIOCHEMICAL AND SPECTROFLUOROMETRIC ANALYSIS OF PYOVERDINE NRPS GENES PVDD AND PVDJ | 第52-55页 |
| 4.4 PYOVERDINE EXPOSURE CAUSES C. ELEGANS DEATH | 第55-56页 |
| 4.5 ROLE OF IRON IN THE PATHOGENICITY OF MB03 AGAINST C. ELEGANS | 第56-58页 |
| 5 DISCUSSION | 第58-60页 |
| 6 CONCLUDING REMARKS AND FUTURE PROSPECTS | 第60-61页 |
| REFERENCES | 第61-68页 |
| LIST OF PUBLICATIONS | 第68-69页 |
| EXTRA ACHIEVEMENTS DURING MY MASTER’S | 第69-70页 |
| APPENDIX I | 第70-71页 |
| APPENDIX II | 第71-72页 |
| ACKNOWLEDGEMENTS | 第72-73页 |
| 附录 | 第73-74页 |
