| Abstract | 第4-5页 |
| Chapter 1. Introduction | 第8-17页 |
| 1.1 Potato is an important crop in the world | 第9-10页 |
| 1.2 Cold-induced sweetening is a serious issue in potato industry | 第10-12页 |
| 1.3 RB-mediated resistance to potato late blight disease | 第12-14页 |
| References | 第14-17页 |
| Chapter 2. Developing cold chipping potato varieties by silencing the vacuolarinvertase gene | 第17-54页 |
| 2.1 Abstract | 第18页 |
| 2.2 Introduction | 第18-23页 |
| 2.3 Materials and methods | 第23-28页 |
| 2.3.1 Plant materials | 第23页 |
| 2.3.2 Silencing constructs for the potato vacuolar acid invertase gene | 第23-24页 |
| 2.3.3 Development and characterization of transgenic plants | 第24-25页 |
| 2.3.4 Measurement of VInv gene transcription | 第25-26页 |
| 2.3.5 Assessment of chip color, tuber sugar contents and chip acrylamide levels | 第26-28页 |
| 2.4 Results | 第28-32页 |
| 2.4.1 Development of VInv gene silencing lines in four potato chipping varieties | 第28-29页 |
| 2.4.2 Sugar contents of the RNAi lines | 第29-30页 |
| 2.4.3 Processing quality of the RNAi lines | 第30-32页 |
| 2.5 Discussion | 第32-37页 |
| References | 第37-41页 |
| Tables | 第41-44页 |
| 附图 | 第44-54页 |
| Chapter 3.Constitutive expression of the RB gene and its impact on potato late blightresistance | 第54-87页 |
| 3.1 Abstract | 第55页 |
| 3.2 Introduction | 第55-59页 |
| 3.3 Materials and methods | 第59-64页 |
| 3.3.1 Plant materials | 第59页 |
| 3.3.2 Southern blot hybridizations | 第59-60页 |
| 3.3.3 Late blight resistance evaluation | 第60-61页 |
| 3.3.4 Quantification of RB gene transcription | 第61-64页 |
| 3.4 Results | 第64-70页 |
| 3.4.1 Copy number of the RB gene among 35S:RB transgenic potato plants | 第64页 |
| 3.4.2 Late blight resistance evaluation of 35S:RB transgenic potato lines | 第64-65页 |
| 3.4.3 No significant correlation was observed among RB transgene copy number and late blight resistance | 第65-67页 |
| 3.4.4 Transcript evaluation of RB gene among different 35S:RB transgenic lines | 第67-70页 |
| 3.5 Discussion | 第70-77页 |
| References | 第77-80页 |
| Tables | 第80-81页 |
| 附图 | 第81-87页 |
| Publication | 第87-88页 |
| Acknowledgement | 第88-90页 |
