| 摘要 | 第4-6页 |
| ABSTRACT | 第6-7页 |
| CHAPTER ONE: LITTERATURE REVIEW | 第12-34页 |
| 1.1 Impact of salt stress in plants | 第12-14页 |
| 1.1.1 Effects of salt stress on plant growth | 第12-13页 |
| 1.1.2 Salinity and ionic toxicity | 第13页 |
| 1.1.3 Effects of salt stress on plant photosynthesis | 第13-14页 |
| 1.1.4 Salinity and oxidative stress | 第14页 |
| 1.2 Plants response to stress | 第14-15页 |
| 1.3 The regulation mechanism of plant response to stress | 第15-18页 |
| 1.3.1 Sensory mechanisms of salt stress and signal transduction | 第15-16页 |
| 1.3.2 Gene regulation of plants in response to salt stress | 第16-17页 |
| 1.3.3 Gene expression profiles of plant response to salt stress | 第17-18页 |
| 1.4 Biology of plant salt tolerance | 第18-20页 |
| 1.4.1 Halophytes and the natural mechanisms for salt tolerance | 第18-19页 |
| 1.4.2 Alters in plant hormone levels | 第19页 |
| 1.4.3 Accumulation of compatible solutes | 第19-20页 |
| 1.5 Heterosis and plant stress tolerance | 第20-22页 |
| 1.5.1 Heterosis | 第20-21页 |
| 1.5.2 Research progress of heterosis | 第21页 |
| 1.5.3 Maize stress tolerance and heterosis | 第21-22页 |
| 1.6 The research progress of L. Chinense | 第22-25页 |
| 1.6.1 Advance of research in L. Chinense | 第22-23页 |
| 1.6.2 L. Chinense and salt stress | 第23-24页 |
| 1.6.3 Carotenoids biosynthesis in plants | 第24-25页 |
| 1.7 Transcription factorsand regulation of genes expression | 第25-30页 |
| 1.7.1 NAC TFs | 第26页 |
| 1.7.2 bZIP TFs | 第26-27页 |
| 1.7.3 WRKY TFs | 第27页 |
| 1.7.4 MYB TFs | 第27-28页 |
| 1.7.5 bHLH TFs | 第28-29页 |
| 1.7.6 HSF TFs | 第29-30页 |
| 1.8 Research objective and significance of this thesis | 第30-34页 |
| 1.8.1 Comparative transcriptome analysis of maize Tianta5 and its parentsunder salt stress condition | 第30-32页 |
| 1.8.2 De novo transcriptome analysis of L. chinense and identification andcharacterization of important functional genes | 第32-34页 |
| CHAPTER TWO: COMPARATIVE TRANSCRIPTOME ANALYSIS OFMAIZE RESPONSE TO SALT STRESS | 第34-60页 |
| 2.1 Materials and methods | 第34-40页 |
| 2.1.1 Maize materials | 第34页 |
| 2.1.2 Evaluation of salt tolerance of the maize varieties | 第34-36页 |
| 2.1.3 High throughput RNA sequencing | 第36-37页 |
| 2.1.4 Data processing and analysis | 第37页 |
| 2.1.5 Sequencing quality assessment | 第37-38页 |
| 2.1.6 Gene expression annotation | 第38页 |
| 2.1.7 Gene Ontology Functional Enrichment Analysis for DEGs | 第38-39页 |
| 2.1.8 KEGG Pathway analysis | 第39页 |
| 2.1.9 Quantitative real-time PCR Verification of the transcriptional data | 第39-40页 |
| 2.2 Results | 第40-50页 |
| 2.2.1 Evaluation of salt tolerance of Tianta5 and its inbred parents | 第40-43页 |
| 2.2.2 Sequencing quality assessment | 第43-47页 |
| 2.2.3 Transcriptome analysis of F1 hybrid and its parents | 第47页 |
| 2.2.4 Identification and Characterization of the DEGs | 第47-48页 |
| 2.2.5 Clustering analysis of the DEGs | 第48-50页 |
| 2.2.6 Gene Ontology Functional Enrichment Analysis for DEGs | 第50页 |
| 2.2.7 KEGG pathway analysis of DEGs | 第50页 |
| 2.3 Discussion | 第50-56页 |
| 2.3.1 Salt treatment | 第50-51页 |
| 2.3.2 Salt stress related GO terms analysis of the DEGs | 第51-54页 |
| 2.3.3 Salt stress related pathway analysis of these DEG | 第54-55页 |
| 2.3.4 Heterosis related genes analysis | 第55-56页 |
| 2.4 Summary | 第56-60页 |
| CHAPTER THREE: DE NOVO CHARACTERIZATION OF LYCIUMCHINENSE MILL. LEAF TRANSCRIPTOME | 第60-92页 |
| 3.1 Materials and methods | 第60-64页 |
| 3.1.1 Plant materials and RNA isolation | 第60-61页 |
| 3.1.2 Illumina sequencing and de novo assembly | 第61页 |
| 3.1.3 Sequence annotation | 第61-62页 |
| 3.1.4 Gene expression levels analysis | 第62页 |
| 3.1.5 Identification of TF genes in L.chinense | 第62页 |
| 3.1.6 Identification of L. chinense unigenes related to carotenoidbiosynthesis | 第62-63页 |
| 3.1.7 qRT-PCR analysis of L. chinense genes involved in carotenoidbiosynthesis | 第63页 |
| 3.1.8 Carotenoid extraction and HPLC | 第63-64页 |
| 3.2 Results | 第64-81页 |
| 3.2.1 Illumina sequencing and de novo assembly | 第64-66页 |
| 3.2.2 Annotation and classification of L. chinense unigenes | 第66-67页 |
| 3.2.3 GO classification | 第67页 |
| 3.2.4 COG classification | 第67-68页 |
| 3.2.5 KEGG pathway mapping | 第68-73页 |
| 3.2.6 Identification of TF genes in L. chinense | 第73-74页 |
| 3.2.7 Gene expression levels analysis | 第74-77页 |
| 3.2.8 Genes related to salt tolerance/stress | 第77页 |
| 3.2.9 Analysis of genes involved in carotenoid biosynthesis pathway | 第77-81页 |
| 3.2.10 Analysis of carotenoid contents in leaf and ripening fruit of L. chinense | 第81页 |
| 3.3 Discussion | 第81-88页 |
| 3.3.1 Plant samples selected for RNA sequencing | 第81-82页 |
| 3.3.2 RNA-seq and de novo assembly | 第82-83页 |
| 3.3.3 Analysis of TF genes in L. chinense | 第83-84页 |
| 3.3.4 Salt tolerance related genes in L. chinense | 第84-88页 |
| 3.4 Summary | 第88-92页 |
| CHAPTER FOUR: ANALYSIS OF TRANSCRIPTION FACTOR GENES INMAIZE UNDER SALT STRESS CONDITIONS | 第92-114页 |
| 4.1 Materials and methods | 第92-96页 |
| 4.1.1 Plant materials and salt stress treatments | 第92页 |
| 4.1.2 Evaluation of salt tolerance in the maize varieties | 第92-93页 |
| 4.1.3 Transcriptome analysis of TF genes and salt-responsive genes | 第93-94页 |
| 4.1.4 Phylogenetic tree construction of HSFs and protein interactionnetworks analysis | 第94页 |
| 4.1.5 RNA extraction and qRT-PCR analysis | 第94-96页 |
| 4.2 Results | 第96-109页 |
| 4.2.1 Evaluation of salt tolerance of the two maize varieties | 第96页 |
| 4.2.2 Identification of TF genes and expression analysis | 第96-98页 |
| 4.2.3 Expression profiles of the TF genes under salt stress conditions | 第98-100页 |
| 4.2.4 Identification of salt-stress responsive genes and TFs | 第100-102页 |
| 4.2.5 Phylogenetic analysis of HSF family and protein interaction networkanalysis | 第102-107页 |
| 4.2.6 Expression analysis of HSF genes under salt stress conditions | 第107-109页 |
| 4.3 Discussion | 第109-113页 |
| 4.3.1 Characterization of expression profiles of TFs in response to saltstress | 第109页 |
| 4.3.2 Salt-stress responsive genes and salt-tolerant genes | 第109-110页 |
| 4.3.3 Critical TFs in plant response to salt stress | 第110-111页 |
| 4.3.4 HSFs and their expression pattern in response to salt stress and theirpotential roles in maize salt tolerance | 第111-113页 |
| 4.4 Summary | 第113-114页 |
| CHAPTER FIVE: CONCLUSION AND FUTURE PROSPECTS | 第114-120页 |
| 5.1 Conclusion | 第114-117页 |
| 5.2 Innovation | 第117-118页 |
| 5.3 Future prospects and Concerns | 第118-120页 |
| REFERENCES | 第120-136页 |
| ADDITIONAL FILES | 第136-188页 |
| PAPER PUBLICATION AND SCIENTIFIC RESEARCH PROJECTS | 第188-190页 |
| ACKNOWLEDGEMENT | 第190-191页 |
