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烟草砷吸收、亚细胞分布及形态的基因型差异与磷酸盐缓解砷毒害的机理研究

ACKNOWLEDGEMENT第7-13页
Abstract第13-15页
摘要第16-19页
Abbreviations第19-20页
Chapter 1 Literature Review第20-29页
    1.1 Background第20-21页
    1.2 Different arsenic forms第21页
    1.3 Arsenic accumulation and uptake dynamics第21-22页
    1.4 Arsenic distribution in subcellular tissues and organs第22-23页
    1.5 Effects of arsenic toxicity in plants第23-26页
        1.5.1 Plant growth parameters第24-25页
        1.5.2 Nutrients uptake第25页
        1.5.3 Photosynthesis and photosynthetic parameters第25-26页
        1.5.4 Lipid peroxidation and antioxidant enzymes第26页
    1.6 Arsenate and phosphate interactions第26-27页
    1.7 Research objectives第27-29页
Chapter 2 The difference in Arsenic uptake and accumulation dynamics among tobacco (Nicotiana tabaccum)genotypes第29-38页
    2.1 Materials and Methods第29-31页
        2.1.1 Sample collection and hydroponic culture第29-30页
        2.1.2 Measurement of arsenic concentration第30页
        2.1.4 Arsenic Sensitivity Index第30页
        2.1.5 Statistical analysis第30-31页
    2.2. Results第31-35页
        2.2.1 Arsenic effects on tissue dry weight第31-32页
        2.2.2 Effects of arsenic on tissue As concentration第32页
        2.2.3 Effect of arsenic on plant As accumulation第32-34页
        2.2.4 Arsenic accumulation dynamics第34-35页
    2.3 Discussion第35-38页
Chapter 3 Arsenic subcellular distribution and speciation in tobacco genotypes differing in As tolerance第38-54页
    3.1 Materials and Methods第39-42页
        3.1.1 Plant Materials第39页
        3.1.2 Seedling growth and hydroponic culture第39-40页
        3.1.3 Separation of tissue fractions第40页
        3.1.4 Arsenic quantification第40-41页
        3.1.5 Arsenic speciation analysis第41-42页
        3.1.6 Chemicals第42页
        3.1.7 Statistics第42页
    3.2 Results第42-50页
        3.2.1 Subcellular distribution of As concentration第42-43页
        3.2.2 Arsenic proportion in different subcellular parts第43-46页
        3.2.3 Arsenic speciation第46-48页
        3.2.4 Percentage distribution of As species第48-50页
    3.4 Discussion第50-54页
Chapter 4 The effects of phosphate on arsenic uptake and toxicity alleviaiton in tobaccogenotypes with differing arsenic tolerances第54-69页
    4.1 Materials and Methods第54-57页
        4.1.1 Hydroponic culture and sample collection第54-55页
        4.1.2 Measurement of the physiological parameters第55页
        4.1.3 Elemental analysis第55-56页
        4.1.4 Determination of lipid peroxidation and enzyme activity第56页
        4.1.5 Statistical analysis第56-57页
    4.2 Results第57-64页
        4.2.1 Plant growth第57页
        4.2.2 Chlorophyll content and photosynthetic parameters第57-59页
        4.2.3 Tissue As and P concentrations第59-61页
        4.2.4 Nutrients content第61-63页
        4.2.5 MDA content and antioxidant enzyme activities第63-64页
    4.3 Discussion第64-69页
Chapter 5 General conclusion第69-71页
References第71-87页
Supplementary tables and figures第87-91页
Publications List第91页

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