| 摘要 | 第1-7页 |
| Abstract | 第7-10页 |
| CONTENTS | 第10-13页 |
| LIST OF FIGURES | 第13-15页 |
| CHAPTER 1 INTRODUCTION | 第15-33页 |
| ·Principle of the optical biosensor | 第17-18页 |
| ·Optical biosensing technology progress | 第18-32页 |
| ·Optical biosensor based on the light absorption | 第18-21页 |
| ·Optical biosensor based on fluorescence | 第21-27页 |
| ·Optical biosensor based on Surface Plasmon Resonance | 第27-28页 |
| ·Optical biosensor based on Surface-enhanced Raman scattering | 第28-29页 |
| ·Optical biosensor based on Chemiluminescence | 第29-32页 |
| ·Scope and organization | 第32-33页 |
| CHAPTER 2 PROTEOLYSIS-MEDIATED PROTECTION OF GOLD NANOPARTICLES FOR SENSITIVE ACTIVITY ASSAY OF PEPTIDASES | 第33-47页 |
| ·Introduction | 第33-34页 |
| ·Experimental | 第34-36页 |
| ·Materials and reagents | 第34页 |
| ·Preparation of peptide-decorated AuNPs | 第34-35页 |
| ·Activity assay of pancreatic elastase | 第35页 |
| ·Absorption spectrum, dynamic light scattering and transmission electron microscopy measurements | 第35-36页 |
| ·Results and discussion | 第36-46页 |
| ·Analytical principle of the biosensing strategy for visual screening of peptidase activity | 第36-37页 |
| ·Typical characteristics of peptidase activity assay | 第37-38页 |
| ·Dynamic light scattering and Transmission electron microscopy characterization of the biosensing strategy for peptidase activity assay | 第38-41页 |
| ·Optimization of peptidase activity assay conditions | 第41-44页 |
| ·Quantitative assay of peptidase activity using the biosensing strategy | 第44-46页 |
| ·Conclusion | 第46-47页 |
| CHAPTER 3 TARGET-MEDIATED CONSECUTIVE ENDONUCLEASE REACTIONS FOR SPECIFIC AND SENSITIVE HOMOGENEOUS FLUORESCENCE ASSAY OF O6-METHYLGUANINE-DNA METHYLTRANSFERASE | 第47-58页 |
| ·Introduction | 第47-48页 |
| ·Experimental | 第48-50页 |
| ·Materials and reagents | 第48-49页 |
| ·Activity assay of MGMT using the developed strategy | 第49页 |
| ·Gel electrophoresis analysis of MGMT | 第49-50页 |
| ·Results and discussion | 第50-57页 |
| ·Probe design and analytical principle | 第50-53页 |
| ·Gel electrophoresis analysis of MGMT | 第53-54页 |
| ·Time optimization of MGMT-mediated endonuclease reaction of PvuII | 第54-55页 |
| ·Quantitative nature of the developed strategy in MGMT activity assay | 第55-57页 |
| ·Conclusion | 第57-58页 |
| CHAPTER 4 GRAPHENE OXIDE-O~6-ALKYLGUANINE CONTAINING DNA NANOCOMPOSITE AS A HOMOGENEOUS ASSAY PLATFORM FOR O~6-METHYLGUANINE-DNA METHYLTRANSFERASE | 第58-69页 |
| ·Introduction | 第58-59页 |
| ·Experimental | 第59-61页 |
| ·Materials and reagents | 第59-60页 |
| ·Synthesis and characterization of GO | 第60页 |
| ·Gel electrophoresis analysis of MGMT | 第60页 |
| ·Activity analysis of O6-methylguanine-DNA methyltransferase | 第60-61页 |
| ·Results and discussion | 第61-68页 |
| ·Design and construction of the biosensor for MGMT activity assay | 第61-62页 |
| ·Typical characteristics of the developed biosensor in MGMT activity assay | 第62-63页 |
| ·Gel electrophoresis analysis of MGMT | 第63-65页 |
| ·Optimization of MGMT activity assay conditions | 第65-66页 |
| ·O6-methylguanine-DNA methyltransferase detection | 第66-68页 |
| ·Conclusion | 第68-69页 |
| CHAPTER 5 HEPATITIS B SURFACE ANTIGEN ASSAY USING SURFACE-ENHANCED RAMAN SCATTERING BASED ON AGGREGATION OF REPORTER-LABELED IMMUNOGOLD NANOPARTICLES | 第69-80页 |
| ·Introduction | 第69-70页 |
| ·Experimental | 第70-72页 |
| ·Materials and reagents | 第70-71页 |
| ·Preparation of Raman reporter labeled immunogold nanoparticles | 第71-72页 |
| ·SERS detection of HBsAg | 第72页 |
| ·Absorption spectrum, transmission electron microscopy and dynamic light scattering analysis | 第72页 |
| ·Results and discussion | 第72-79页 |
| ·Analytical Principle of HBsAg-Based SERS Biosensor | 第72-73页 |
| ·Surface enhance Raman scattering characteristics of the biosensor | 第73-74页 |
| ·Surface plasmon absorption measurement characteristics of the biosensor | 第74-75页 |
| ·Transmission electron microscopy and Dynamic light scattering characterization of the biosensing strategy for HBsAg assay | 第75-78页 |
| ·Hepatitis B surface antigen detection | 第78-79页 |
| ·Conclusion | 第79-80页 |
| CONCLUSION | 第80-82页 |
| REFERENCES | 第82-100页 |
| PUBLICATIONS | 第100-101页 |
| ACKNOWLEDGEMENTS | 第101页 |
