| 摘要 | 第6-8页 |
| Abstract | 第8-9页 |
| Abbreviation | 第19-22页 |
| CHAPTER Ⅰ Introduction | 第22-33页 |
| 1.1 Cotton | 第22页 |
| 1.2 Leaf senescence | 第22-29页 |
| 1.2.1 Analysis and regulation of leaf senescence | 第24-25页 |
| 1.2.2 Molecular approaches for leaf senescence | 第25-26页 |
| 1.2.3 Molecular genetics regulation of leaf senescence | 第26-27页 |
| 1.2.4 Onset of leaf senescence | 第27页 |
| 1.2.5 Environmental factors and hormones regulating leaf senescence | 第27-29页 |
| 1.2.5.1 Environmental factors regulating leaf senescence | 第27-28页 |
| 1.2.5.2 Phytohormones pathways in leaf senescence | 第28-29页 |
| 1.3 Cot_genes family | 第29-32页 |
| 1.3.1 General information of Cot_genes family | 第29-31页 |
| 1.3.2 Thioredoxins(TRX)gene family | 第31页 |
| 1.3.3 Ribosomal large protein(RPL2)gene | 第31-32页 |
| 1.4 Research Objectives | 第32-33页 |
| CHAPTER Ⅱ Functional analysis of nine cotton genes related to leaf senescence in Gossypium hirsutum L | 第33-46页 |
| 2.1 Materials and methods | 第33-35页 |
| 2.1.1 Phylogenetic analysis | 第33页 |
| 2.1.2 Plant material and growth conditions | 第33页 |
| 2.1.3 Abiotic stress and phytohormone treatments | 第33-34页 |
| 2.1.4 Extraction total RNA,and cDNA synthesis | 第34页 |
| 2.1.5 Cloning of nine cotton genes,DNA and cDNA sequences | 第34页 |
| 2.1.6 Quantitative RT-PCR assays | 第34-35页 |
| 2.2 Results | 第35-42页 |
| 2.2.1 Phylogenetic analysis of cotton genes | 第35-36页 |
| 2.2.2 Profiling specific tissue | 第36-37页 |
| 2.2.3 Leaf development stages | 第37-38页 |
| 2.2.4 Expression of the cotton genes under different phytohormones treatment conditions | 第38-40页 |
| 2.2.5 Expression of cotton genes under abiotic stress | 第40-41页 |
| 2.2.6 Expression of cotton genes under wounding(W) | 第41-42页 |
| 2.3 Discussion | 第42-46页 |
| CHAPTER Ⅲ Characterization of TRX gene family for leaf senescence and abiotic stress in upland cotton | 第46-64页 |
| 3.1 Materials and methods | 第46-49页 |
| 3.1.1 Identification of TRX proteins in cotton | 第46页 |
| 3.1.2 Database searches and phylogenetic analysis | 第46页 |
| 3.1.3 Phylogenetic tree | 第46-47页 |
| 3.1.4 Chromosomal locations and gene duplication events | 第47页 |
| 3.1.5 Gene ontology and protein localization | 第47页 |
| 3.1.6 Promoter analysis | 第47页 |
| 3.1.7 Plant material and growth conditions | 第47-48页 |
| 3.1.8 Phytohormones and abiotic treatment | 第48页 |
| 3.1.9 RNA isolation and cDNA synthesis | 第48-49页 |
| 3.1.10 Quantitative RT-PCR assays | 第49页 |
| 3.2 Results | 第49-60页 |
| 3.2.1 Classification of GhTRX proteins | 第49-50页 |
| 3.2.2 Phylogenetic analysis of GhTRX | 第50-52页 |
| 3.2.3 Phylogenetic analysis of the TRX genes family | 第52-53页 |
| 3.2.4 Chromosomal locations of the TRX genes | 第53-54页 |
| 3.2.5 Duplication and evolution analysis of the GhTRX genes | 第54-55页 |
| 3.2.6 Gene ontology and protein localization | 第55-56页 |
| 3.2.7 Promoter analysis | 第56-57页 |
| 3.2.8 Profiling specific tissue | 第57-58页 |
| 3.2.9 Leaf development stages | 第58页 |
| 3.2.10 Gene expression patterns under different treatments | 第58-60页 |
| 3.3 Discussion | 第60-64页 |
| CHAPTER Ⅳ Overexpression of CDSP32(GhTRX134)and RPL2 from cotton enhances tolerance to abiotic stress in Arabidopsis | 第64-90页 |
| 4.1 Materials and Methods | 第64-69页 |
| 4.1.1 Plant materials and growth conditions | 第64页 |
| 4.1.2 RNA extraction,cDNA synthesis and DNA isolation | 第64页 |
| 4.1.3 Amplification of CDSP32 and RPL2 genes | 第64-65页 |
| 4.1.4 Quantitative RT-PCR assays | 第65页 |
| 4.1.5 Create of CDSP32 and RPL2 transgenic Arabidopsis plants | 第65-67页 |
| 4.1.5.1 Construction of CDSP32(GhTRX134)and RPL2 plasmids | 第65页 |
| 4.1.5.2 In-Fusion cloning reaction | 第65-66页 |
| 4.1.5.3 Transformation of Arabidopsis | 第66-67页 |
| 4.1.5.4 Growth transgenic plants under stress treatments | 第67页 |
| 4.1.6 Plasmid constructions for VIGS Assay and vector inoculation | 第67-68页 |
| 4.1.7.Transactivation analysis of GhTRX134 and GhRPL | 第68页 |
| 4.1.8 Expression of stress-responsive genes in transgenic of GhTRX134 and GhRPL2 plants and Wt under abiotic stress | 第68页 |
| 4.1.9 Measurements of proline content,enzymes activity,and chlorophyll content in transgenic and Wt plants | 第68-69页 |
| 4.1.10 Experimental design and statistical analysis | 第69页 |
| 4.2 Result | 第69-85页 |
| 4.2.1 Cloning and Characterization of GhTRX134(CDSP32)and GhRPL | 第69-70页 |
| 4.2.2 Generation and characterization of CDSP32(GhTRX134)and RPL2overexpressing lines | 第70-76页 |
| 4.2.2.1 Expression and phenotypic analysis of GhTRX134 overexpression during abiotic stresses | 第70-73页 |
| 4.2.2.2 Expression and phenotypic analysis of GhRPL2 overexpression during abiotic stresses | 第73-76页 |
| 4.2.3 GhTRX134 and GhRPL2 played a positive role in drought stress in cotton(VIGS) | 第76-77页 |
| 4.2.4 Transactivation analysis of GhTRX134 and Gh RPL | 第77-78页 |
| 4.2.5 Expression of stress-responsive genes in transgenic and Wt plants under abiotic stress | 第78-82页 |
| 4.2.5.1 Expression of stress-responsive genes in GhTRX134 overexpression and Wt plants | 第79-80页 |
| 4.2.5.2 Expression of stress-responsive genes in GhRPL2overexpression and Wt plants | 第80-82页 |
| 4.2.6 Measurements of proline content,enzyme activity,and chlorophyll content in transgenic lines and Wt plants | 第82-85页 |
| 4.2.6.1 Measurements of proline content,enzyme activity,and chlorophyll content in Gh TRX134overexpression and Wt plants | 第82-83页 |
| 4.2.6.2 Measurements of proline content,enzyme activity,and chlorophyll content in Gh RPL2overexpression and Wt plants | 第83-85页 |
| 4.3 Discussion | 第85-90页 |
| CONCLUSION | 第90-91页 |
| REFERENCES | 第91-107页 |
| SUPPLEMENTARY DATA | 第107-121页 |
| Acknowledgements | 第121-122页 |
| Curriculum Vitae(作者简历) | 第122-123页 |
