| 摘要 | 第5-6页 |
| Abstract | 第6-7页 |
| CHAPTER ONE INTRODUCTION | 第14-20页 |
| 1.1 Background and motivation | 第14页 |
| 1.2 Literature Review | 第14-18页 |
| 1.2.1 Pretreatment of lignocellulosic biomass | 第15-17页 |
| 1.2.2 Immobilization of yeast cells | 第17页 |
| 1.2.3 Response surface methodology | 第17-18页 |
| 1.3 Existing Problem | 第18页 |
| 1.4 Aims | 第18页 |
| 1.5 objectives | 第18-20页 |
| CHAPTER TWO Microwave-Alkali Based Pretreatment of Lignocellulosic Biomass for Bioethanol Production | 第20-34页 |
| 2.1 Introduction | 第20页 |
| 2.2 Materials and Methods | 第20-23页 |
| 2.2.1 Raw material | 第20-21页 |
| 2.2.2 The feasibility of the microwave-assisted alkali pretreatment in lignocellulosic biomass | 第21页 |
| 2.2.3 Optimization of pretreatment conditions | 第21页 |
| 2.2.4 RS and SB chemical composition and sugar analysis | 第21-22页 |
| 2.2.5 Enzymatic hydrolysis | 第22-23页 |
| 2.3 Results and discussion | 第23-32页 |
| 2.3.1 The feasibility of the microwave-assisted alkali pretreatment in lignocellulosic biomass | 第23-25页 |
| 2.3.2 Optimization of pretreatment condition | 第25-30页 |
| 2.3.3 Enzyme hydrolysis of pretreated lignocellulosic biomasses | 第30页 |
| 2.3.4 Optimization of enzyme loading | 第30-32页 |
| 2.4 Conclusion | 第32-34页 |
| CHAPTER THREE Cellulase Enzyme Production by Aspergillus niger | 第34-48页 |
| 3.1 Introduction | 第34页 |
| 3.2 Materials and Method | 第34-39页 |
| 3.2.1 Substrate and pretreatment | 第34-35页 |
| 3.2.2 Microorganism and culture medium | 第35页 |
| 3.2.3 Fermentation and Cellulase-system production | 第35-36页 |
| 3.2.4 Nutritional factors study | 第36-37页 |
| 3.2.5 Enzyme Production | 第37页 |
| 3.2.6 Analytical methods | 第37-39页 |
| 3.3 Results and Discussion | 第39-46页 |
| 3.3.1 Effect of different fermentation methods on cellulase production | 第40-43页 |
| 3.3.2 Influence of Wheat Bran addition on Cellulase Production | 第43页 |
| 3.3.3 Study on effect of nutrient-limited system | 第43-44页 |
| 3.3.4 Characterization of crude cellulase enzyme | 第44-46页 |
| 3.4 Conclusion | 第46-48页 |
| CHAPTER FOUR Immobilized and Free Yeast Cell Fermentation | 第48-58页 |
| 4.1 Introduction | 第48页 |
| 4.2 Material and Methods | 第48-52页 |
| 4.2.1 Feedstock preparation and pretreatment procedures | 第48页 |
| 4.2.2 Microwave-alkali assisted pretreatment | 第48-49页 |
| 4.2.3 Microorganism and Inoculum preparation | 第49页 |
| 4.2.4 Yeast immobilization | 第49-50页 |
| 4.2.5 Microencapsulation efficiency | 第50页 |
| 4.2.6 Microcapsule size | 第50页 |
| 4.2.7 Estimation of Immobilized Cells | 第50-51页 |
| 4.2.8 Bioethanol Fermentation by Delignified Cellulosic Material Supported Biocatalyst | 第51页 |
| 4.2.9 Repeated Bateh Fermentations for ethanol production | 第51-52页 |
| 4.3 Results and Discussion | 第52-57页 |
| 4.3.1 Morphology of Saccharomyces cerevisiae in solid medium and its growth curve | 第52-53页 |
| 4.3.2 Microencapsulation establishment efficiency | 第53-54页 |
| 4.3.3 Microcapsule size analysis | 第54页 |
| 4.3.4 Bioethanol production by immobilized yeast in a batch system | 第54-56页 |
| 4.3.5 The application of immobilized yeast in multiple batch fermentation cycles | 第56-57页 |
| 4.4 Conclusion | 第57-58页 |
| CHAPTER FIVE RESPONSE SURFACE METHODOLOGY | 第58-74页 |
| 5.1 Introduction | 第58页 |
| 5.2 Material and Methods | 第58-59页 |
| 5.2.1 Experimental design for cellulase production and enzymatic hydrolysis | 第58-59页 |
| 5.2.2 Experimental design for ethanol production optimization | 第59页 |
| 5.2.3 Validation of the experimental model | 第59页 |
| 5.3 Results and Discussion | 第59-71页 |
| 5.3.1 Optimization of Cellulase Production | 第59-63页 |
| 5.3.2 Optimization of Enzymatic saccharification | 第63-67页 |
| 5.3.3 Validation of Models | 第67-68页 |
| 5.3.4 Optimization of Bioethanol Production | 第68-71页 |
| 5.4 Conclusion | 第71-74页 |
| CHAPTER SIX CONCLUSIONS AND SUGGESTIONS | 第74-78页 |
| 6.1 Conclusion | 第74-76页 |
| 6.1.1 Microwave-Alkali Based Pretreatment of Lignocellulosic Biomass for Bioethan | 第74-75页 |
| 6.1.2 Cellulase Enzyme Production by Aspergillus niger | 第75页 |
| 6.1.3 Immobilized and Free Yeast Cell Fermentation | 第75页 |
| 6.1.4 A statistical optimization-Response Surface Methodology | 第75-76页 |
| 6.2 Suggestions | 第76-78页 |
| Acknowledgement | 第78-80页 |
| References | 第80-86页 |
| Publications | 第86-87页 |
| Nomenclature | 第87-88页 |
| Curriculum Vitae | 第88页 |
