| 摘要 | 第6-8页 |
| Abstract | 第8-10页 |
| Ⅰ. Advancement of studies on protecting peanut from aflatoxins contamination | 第11-31页 |
| 1. Introduction | 第11-13页 |
| 2. Detrimental of Aflatoxin Contamination | 第13-16页 |
| 2.1 Aspergillus flavus and aflatoxin | 第13-14页 |
| 2.2 Impact on food security and economy | 第14-16页 |
| 3. Basic Researches on Peanut Resisting Aflatoxin contamination | 第16-21页 |
| 3.1 Aspergillus flavus genomics at the process of invation | 第16-17页 |
| 3.2 Factors affect the production of aflatoxin | 第17-19页 |
| 3.3 Common mechanisms of plant disease resistance | 第19-20页 |
| 3.4 Selection for aflatoxin contamination resistance traits | 第20-21页 |
| 4. Measures used for protecting peanut from Aflatoxin contamination | 第21-25页 |
| 4.1 Agronomical prevention in the field | 第21-22页 |
| 4.2 Utilization of biological control | 第22-23页 |
| 4.3 Enhancing resistance of host plant by breeding and genetic engineering | 第23页 |
| 4.4 Applying the achievement of molecular study of peanut in all directions | 第23-25页 |
| 5. Prospect and conclusion | 第25-26页 |
| Reference | 第26-31页 |
| Ⅱ. Cloning and Characterization of a NB-ARC gene-AhRAF4 induced by Aspergillus flavusinfection in peanut | 第31-55页 |
| 1. Introduction | 第32-34页 |
| 2. Materials and methods | 第34-39页 |
| 2.1 Materials and treatments | 第34-35页 |
| 2.2 RNA extractions and semiquantitative RT-PCR | 第35页 |
| 2.3 Microarray data analysis | 第35-36页 |
| 2.4 Gene cloning by RACE | 第36-37页 |
| 2.5 Amplification of AhRAF4 from cDNA and gDNA sequences | 第37-38页 |
| 2.6 Bioinformatic analysis of AhRAF4 | 第38页 |
| 2.7 Real-time PCR | 第38-39页 |
| 3. Results | 第39-49页 |
| 3.1 Cloning of AhRAF4 gene induced by A.flavus infection | 第39-40页 |
| 3.2 Analysis of the composition and structure of AhRAF4 gene | 第40-43页 |
| 3.3 Physical-chemical characteristics and functional domains of AhRAF4 | 第43-46页 |
| 3.4 Expression analysis of AhRAF4 gene | 第46-49页 |
| 4. Discussion | 第49-51页 |
| Reference | 第51-55页 |
| Ⅲ. Identification and characterization of a NBS-LRR gene AhRAF42 down-regulated byAspergillus flavus infection in peanuts | 第55-82页 |
| 1. Introduction | 第55-58页 |
| 2. Materials and methods | 第58-63页 |
| 2.1 Preparing for fungal strains | 第58页 |
| 2.2 Plant materials and treatments | 第58-59页 |
| 2.3 RNA extractions and semiquantitative RT-PCR | 第59-60页 |
| 2.4 Gene cloning by RACE | 第60-62页 |
| 2.5. Validation of the gene expression in R/S plants by real-time PCR | 第62页 |
| 2.6 Subcellular Localization | 第62-63页 |
| 3. Results | 第63-75页 |
| 3.1 Isolation and structural analysis of R gene homologue AhRAF42 | 第63-68页 |
| 3.2 Physical-chemical characteristics of peanut AhRAF42 | 第68-70页 |
| 3.3 Expression analysis of AhRAF42 gene | 第70-73页 |
| 3.4 Localization of AhRAF42 in onion epidermal cells | 第73-75页 |
| 4. Discussion | 第75-78页 |
| Reference | 第78-82页 |
| 致谢 | 第82页 |
