| Acknowledgement | 第4-6页 |
| 摘要 | 第6-9页 |
| Abstract | 第9-10页 |
| List of Abbreviations | 第12-16页 |
| Chapter 1: Literature review | 第16-28页 |
| 1.1 Drug Discovery from Nature | 第16-23页 |
| 1.1.1 Drug discovery from Marine Life | 第17-19页 |
| 1.1.2 Drug Discovery from Marine microorganisms | 第19-23页 |
| 1.1.2.1 Drug Discovery from Marine Hydrothermal vent Fungi | 第19-20页 |
| 1.1.2.2 Drug Discovery from Marine Actinobacteria | 第20-22页 |
| 1.1.2.3 Drug Discovery from marine microorganisms by metal-stress Technique | 第22-23页 |
| 1.2 Infectious Diseases | 第23-24页 |
| 1.3 Problems in Natural Product Research | 第24-26页 |
| 1.4 Aims | 第26-28页 |
| Chapter 2:Production of an antibiotic enterocin from a marine actinobacteria strainH1003 by metal-stress Technique with enhanced enrichment using response surfacemethodology | 第28-55页 |
| 2.1 Equipment's and materials | 第29-31页 |
| 2.1.1 Equipment's | 第29-30页 |
| 2.1.2 Materials | 第30-31页 |
| 2.2 Experimental methods | 第31-34页 |
| 2.2.1 Soil sample collection | 第31页 |
| 2.2.2 Isolation and Storage of H-1003 strain | 第31-32页 |
| 2.2.3 Normal and Metal stress Cultivation | 第32页 |
| 2.2.4 Chemical screening by HPLC Analysis and Identification of Stress Metabolite | 第32页 |
| 2.2.5 Extraction and isolation | 第32-33页 |
| 2.2.6 Optimization of medium composition by response surface methodology | 第33-34页 |
| 2.2.7 Bioassay | 第34页 |
| 2.3 Results and Discussion | 第34-53页 |
| 2.3.1 Bioactive-guided screening of metal-stressed cultivations | 第34-35页 |
| 2.3.2 Chemical Investigations of Metal Treated and Untreated Extracts by HPLC | 第35-38页 |
| 2.3.3 Identification and Structure Determination of Metal-induced Secondary Metabolites | 第38-44页 |
| 2.3.3.1 Structure determination | 第39-44页 |
| 2.3.4 Antimicrobial Activity of enterocin | 第44页 |
| 2.3.5 Factors affecting enhancement of enterocin production | 第44-49页 |
| 2.3.6 Response Surface Optimization for enterocin | 第49-53页 |
| 2.4 Summary | 第53-55页 |
| Chapter 3:Isolation and purification of bioactive secondary metabolites from marinehydrothermal vent fungi strain XZ-1 | 第55-71页 |
| 3.1 Equipment's and materials | 第56页 |
| 3.2 Experimental methods | 第56-58页 |
| 3.2.1 Isolation and cultivation | 第56页 |
| 3.2.2 Extraction | 第56-57页 |
| 3.2.3 Mounting of fungal strain XZ-1 | 第57页 |
| 3.2.4 Chemical screening of partially purified compounds | 第57页 |
| 3.2.5 Antimicrobial assay | 第57-58页 |
| 3.2.5.1 Refreshing isolates | 第58页 |
| 3.2.5.2 MIC and MBC | 第58页 |
| 3.3 Results and Discussion | 第58-70页 |
| 3.3.1 Identification of strain XZ-1 | 第58-59页 |
| 3.3.2 Bioactive-guided screening of partially purified compounds | 第59-62页 |
| 3.3.2.1 Antimicrobial activity of partially purified extracts | 第59-62页 |
| 3.3.3 Chemical Screening by HPLC analysis and identification of secondary metabolites | 第62-64页 |
| 3.3.4 Structure identification of secondary metabolites | 第64-70页 |
| 3.3.4.1 Structure determination | 第65-70页 |
| 3.4 Summary | 第70-71页 |
| Chapter 4: Summary and Conclusion | 第71-74页 |
| References | 第74-80页 |
| Appendix | 第80-95页 |
| Resume | 第95页 |
